Analyzing the Role of Adult Hippocampal Neurogenesis in Contextual Fear Memory

分析成人海马神经发生在情境恐惧记忆中的作用

• 批准号: 8090586
• 负责人: MICHAEL R DREW
• 金额: $ 24.71万
• 依托单位: UNIVERSITY OF TEXAS AT AUSTIN
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-08-25 至 2013-07-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8090586
• 关键词: Ablation; Adult; Affect; Age; Animals; Antidepressive Agents; Area; Award; Behavior; Behavioral; Biological Models; Biology; Brain; Cell Proliferation; Cells; Consultations; Cranial Irradiation; Cytoplasmic Granules; Data; Development; Diphtheria Toxin; Dose; Drug effect disorder; Emotional disorder; Environment; Ethical Issues; Etiology; Exhibits; Fright; Genetic; Goals; Hippocampus (Brain); Immediate-Early Genes; Impairment; Knowledge; Laboratories; Learning; Life; Literature; Memory; Mentorship; Methods; Molecular; Molecular Genetics; Mus; Neurons; Neurosciences; Organism; Performance; Physiologic pulse; Procedures; Process; Psychiatry; Psychologist; Quantitative Microscopy; Research; Research Personnel; Research Support; Research Training; Retrieval; Rodent; Role; Science; Shock; Staging; Synapses; System; Testing; Therapeutic; Time; Toxin; Training; Universities; Wild Type Mouse; adult neurogenesis; aged; base; behavior test; career; classical conditioning; clinical practice; cohort; conditioned fear; conditioning; diphtheria toxin receptor; experience; genetic manipulation; insight; irradiation; killings; meetings; memory acquisition; memory process; memory retrieval; neurogenesis; novel; programs; promoter; recombinase; research study; response; skills; tool; translational neuroscience; young adult

This training and research award will prepare the candidate for an independent career as a molecular and behavioral neuroscientist using rigorous behavioral tesfing and genefic manipulafions to analyze the brain circuits underlying learning and memory. The candidate is an experimental psychologist with significant experience in the area of animal learning. In the training (K99) phase of the award, which is now complete, the candidate (1) developed laboratory skills in molecular genetics and quantitative microscopy; (2) expanded his knowledge of molecular genefics and translational neuroscience so that he can independently develop novel scientific ideas, integrate his research findings with exisfing knowledge, and relate them to clinical practice; (3) developed a strong understanding of the ethical issues inherent in the pracfice of science; and (4) secured a tenure-track faculty position at a major research university. The independent (ROO) phase of the award will provide support as the candidate establishes an independent research program aimed at elucidafing mechanisms through which adult hippocampal neurogenesis contributes to learning and memory. The adult hippocampus retains the ability to generate neurons, and a rapidly growing literature documents that adult-born neurons are funcfionally significant. The candidate has shown, for instance, that blocking hippocampal neurogenesis using irradiafion or an inducible genefic method impairs contextual fear condifioning, a ubiquitous form of learning in which organisms acquire fear of environments that predict harm. Using contextual fear conditioning as a model system, the proposed research will use targeted irradiation and genetic manipulations to analyze how adult-born neurons contribute to acquisition, retenfion, and retrieval of memories.

该培训和研究奖将使候选人成为分子和 行为神经科学家使用严格的行为tesfing和Genefic manipulafions分析大脑 电路的学习和记忆。候选人是一名实验心理学家 在动物学习领域的经验。在该奖项的培训阶段（K99）中，该奖项现已完成， 候选人（1）开发了分子遗传学和定量显微镜的实验室技能； （2） 扩大了他对分子基因和转化神经科学的了解，以便他可以独立 发展新颖的科学思想，将他的研究发现与知识的知识相结合，并将其与 临床实践； （3）对伦理问题的深刻理解 科学; （4）在一所大型研究大学获得了终身教师职位。独立 （ROO）奖励阶段将提供支持，因为候选人建立了一项独立研究 旨在使用成年海马神经发生有助于征服机制的计划 学习和记忆。成年海马保留了产生神经元的能力，并迅速增长 文献记录了成年神经元在函数上具有重要意义。候选人已显示 实例，使用辐照度或诱导基因FENFAIC方法阻止海马神经发生 情境恐惧置换，一种无处不在的学习形式 预测危害。将上下文恐惧条件作为模型系统，拟议的研究将使用 有针对性的辐照和遗传操作，以分析成年神经元如何贡献获得的收购， 重述，并取回回忆。