Core A: Lipidomics

核心 A：脂质组学

基本信息

批准号：
9072009
负责人：
ALICJA BIELAWSKA
金额：
$ 26.6万
依托单位：
MEDICAL UNIVERSITY OF SOUTH CAROLINA
依托单位国家：
美国
项目类别：
财政年份：
2016
资助国家：
美国
起止时间：
2016-05-01 至 2021-04-30
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/9072009
关键词：
Antineoplastic Agents Apoptosis Biocompatible Materials Biological Biological Assay Biology Blood specimen Brain Cancer Biology Cancer Center Cell Culture Techniques Cell Death Cells Ceramidase Ceramides Chemicals Coupled Culture Media Data Data Analyses Development Drug Targeting Drug or chemical Tissue Distribution Educational workshop Enzyme Inhibitor Drugs Enzyme Inhibitors Enzymes Experimental Designs Functional disorder Future Generations Goals Growth High Pressure Liquid Chromatography Human Human Resources Image In Vitro International Kidney Label Lipid Chemistry Lipids Liquid substance Liver Malignant Neoplasms Mass Spectrum Analysis Measurement Measures Mentors Metabolic Metabolism Methodology Methods Molecular Molecular Analysis Molecular Profiling Neoplasm Metastasis Normal Cell Normal tissue morphology Organ Organelles Pathology Pathway interactions Pharmaceutical Preparations Plasma Preparation Procedures Protocols documentation Radioactive Radiolabeled Reagent Regulation Research Research Personnel Resource Sharing Resources Role Serum Services Signal Transduction Site Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization Sphingolipids Sphingomyelinase Sphingomyelins Stereoisomer Structure Technology Therapeutic Tissues Training Vertebral column Work analog angiogenesis base biobank cancer cell cancer therapy comparative design dihydroceramide drug distribution enzyme activity in vitro activity in vivo inhibitor/antagonist inorganic phosphate interest lipid mediator lipid metabolism liquid chromatography mass spectrometry metabolomics novel programs radiotracer research study response tandem mass spectrometry therapeutic target tissue preparation tool tumor tumor progression tumor xenograft

项目摘要

SUMMARY The purpose of the Lipidomics Core in the PPG is to provide intellectual and physical resources (particularly state-of-the-art mass spectrometry analysis of sphingolipids and synthetic molecular tools) to enhance understanding of the role of bioactive lipids in cancer biology with the goal to discover future therapeutics. The diversity of bioactive lipids and their interconnected metabolism generates a network of pathways regulating intra- and inter-cellular signaling and function. Dysfunctions in these pathways contribute to the pathobiology of cancer progression and metastasis. These considerations have necessitated the development of lipid chemistry and analysis. The Lipidomics Core was created based on unique expertise of the key personnel in lipid chemistry, analysis, and metabolism. This Core has developed into an institutional, national, and international resource in the emerging fields of lipidomics and lipid chemical biology. Core services include: 1) Providing qualitative and quantitative analysis of lipid components from different biological materials (cells, tissue, and biological fluids), primarily employing high performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) technology—the Core currently provides quantitative analysis of more than 300 distinct lipid molecular species at a basic metabolomic level; 2) Developing and providing synthetic molecular tools to study lipid metabolism (e.g., functionalized and fluorescent ceramides, site-specific radioactive sphingolipids, 17C-sphingolipids) and diversified synthetic lipids and analogs for cellular, in vitro, and in vivo studies (e.g., organelle-targeted sphingolipids and organelle-targeted inhibitors of sphingolipid metabolizing enzymes); 3) Providing direct MALDI-MS tissue analysis to visualize the distribution of lipid based anti-cancer drugs and their molecular effects on sphingolipid components; and 4) Assisting PPG investigators in experimental design, selection of lipids of interest, and interpretation of analytical results.

概括 PPG中脂质组核心的目的是提供智力和物理资源（部分地鞘脂和合成分子工具的最先进的质谱分析）以增强了解生物活性脂质在癌症生物学中的作用，目的是发现未来的疗法。这生物活性脂质的多样性及其相互联系的代谢产生调节的途径网络细胞内和细胞间信号传导和功能。这些途径中的功能障碍有助于病理生物学癌症进展和转移。这些考虑有必要发展脂质化学和分析。脂质组学核心是基于关键人员的独特专业知识而创建的脂质化学，分析和代谢。该核心已发展成为机构，国家和脂质组学和脂质化学生物学新兴领域的国际资源。核心服务包括：1）提供不同生物材料（细胞，细胞，细胞的脂质成分的定性和定量分析）组织和生物液），主要采用高性能液相色谱量质量光谱法（HPLC-MS/MS）技术 - 核心当前提供了300多个的定量分析在基本代谢组水平上不同的脂质分子物种； 2）开发和提供合成分子研究脂质代谢的工具（例如功能化和荧光神经酰胺，特定于位点的放射性鞘脂，17c-sphingolipids）和多样化的合成脂质和细胞，体外和体内的类似物研究（例如，靶向细胞器的鞘脂和细胞器靶向鞘脂代谢的抑制剂酶）; 3）提供直接的MALDI-MS组织分析以可视化脂质抗癌的分布药物及其对鞘脂成分的分子作用； 4）协助PPG调查人员实验设计，感兴趣的脂质的选择以及分析结果的解释。