SC COBRE: LIPIDOMICS CORE

SC COBRE：脂质组学核心

• 批准号: 7959962
• 负责人: ALICJA BIELAWSKA
• 金额: $ 43.8万
• 依托单位: MEDICAL UNIVERSITY OF SOUTH CAROLINA
• 依托单位国家: 美国
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-07-01 至 2010-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7959962
• 关键词: Aging; Award; Biocompatible Materials; Biological; Biology; Cells; Centers of Research Excellence; Ceramides; Communities; Computer Retrieval of Information on Scientific Projects Database; Development; Disease; Enzymes; Experimental Designs; Extramural Activities; Fee-for-Service Plans; Functional disorder; Funding; Glucosylceramides; Glycerol; Goals; Grant; High Pressure Liquid Chromatography; Human Resources; In Vitro; Inflammation; Institution; International; Lipid Chemistry; Lipids; Liquid substance; Metabolism; Molecular; Neoplasm Metastasis; Organelles; Pathogenesis; Pathway interactions; Program Research Project Grants; Radioactive; Research; Research Personnel; Resources; Signal Transduction; Site; Source; Sphingolipids; Sphingomyelins; Staging; Technology; Tissues; Training; United States National Institutes of Health; analog; base; in vivo; inhibitor/antagonist; inorganic phosphate; instrument; interest; lipid metabolism; metabolomics; success; tandem mass spectrometry; tool; tumor progression

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The diversity ofr bioactive lipids and their interconnected metabolism provides a network of pathways regulating intra- and inter-cellular signaling and function. Dysfunction in these pathways contribute to the pathobiology of specific diseases such as cancer progression and metastasis, accelerated aging, inflammation, and fungal pathogenesis. This emphasized needs for developing lipid chemistry and analysis. The Lipidomics Core was created based on unique expertise of the key personnel in lipid chemistry, analysis and biology has evolved into an institutional, national, and even international resource that serves the needs of the research community in the field of Sphinglipids. The core provides conceptual and practical training in various aspects of lipidology, qualitative and quantitative analysis of lipid components from different biological materials (cells, tissue, biological fluids), synthetic molecular tools to study lipid metabolism (funtionalized and fluorescent ceramides, site-specific radioactive sphingolipids), diversified synthetic lipids and analogs for cellular, in vitro, and in vivo studies (organelle-targeting sphingolipids and organelle-targeting inhibitors of sphingolipid metabolizing enzymes), and assists investigators in experimental design, selection of lipid of interest and interpretation of the analytical results. Analytical approach is based on High Performance Liquid Chromatography-Tandem Mass Spectrometry (LC-MS) technology. Currently, we provide simultaneous analysis of sphingoid bases and their phosphates, ceramide species and their phosphates, sphingomyelin species and diacyl-glycerol species. Glucosylceramide is under a final developmental stage. Our goal is to reach metabolomic profile of sphingolipids. The core has been instrumental to the success of the COBRE PIs and in obtaining extramural funding for the two Program Project Grants and the award of a competitive shared instrument grant from NIH, which supported our second MS instrument. The core is engaged in several collaborative projects and provides also a paid fee-for-service.

该副本是利用众多研究子项目之一 由NIH/NCRR资助的中心赠款提供的资源。子弹和 调查员（PI）可能已经从其他NIH来源获得了主要资金， 因此可以在其他清晰的条目中代表。列出的机构是 对于中心，这不一定是调查员的机构。 生物活性脂质的多样性及其相互联系的代谢提供了调节细胞内和细胞间信号传导和功能的途径网络。 这些途径的功能障碍有助于特定疾病的病理生物学，例如癌症进展和转移，加速衰老，炎症和真菌发病机理。 这强调了开发脂质化学和分析的需求。 脂质组学核心是基于脂质化学，分析和生物学主要人员的独特专业知识而创建的，该专业人数已演变为一种满足Sphinglipids领域研究社区需求的机构，国家甚至国际资源。 The core provides conceptual and practical training in various aspects of lipidology, qualitative and quantitative analysis of lipid components from different biological materials (cells, tissue, biological fluids), synthetic molecular tools to study lipid metabolism (funtionalized and fluorescent ceramides, site-specific radioactive sphingolipids), diversified synthetic lipids and analogs for cellular, in vitro, and体内研究（细胞器靶向鞘脂和鞘脂代谢酶的细胞器靶向抑制剂），并协助研究者进行实验设计，感兴趣的脂质选择和分析结果的解释。 分析方法基于高性能液相色谱量质谱法（LC-MS）技术。 目前，我们同时分析鞘脂碱及其磷酸盐，神经酰胺物种及其磷酸盐，鞘磷脂种类和二酰基甘油物种。 葡萄糖基酰胺正处于最终发育阶段。 我们的目标是达到鞘脂的代谢组分。 该核心对cobre PI的成功和获得两个计划项目赠款的校外资金以及授予NIH的竞争性共享工具赠款的核心作用，该资助支持了我们的第二个MS工具。 核心参与了几个协作项目，还提供了付费费用。