Single Cell Phenotyping Core

单细胞表型核心

基本信息

批准号：
9171180
负责人：
SHERRY L THORNTON
金额：
$ 8.99万
依托单位：
CINCINNATI CHILDRENS HOSP MED CTR
依托单位国家：
美国
项目类别：
财政年份：
2016
资助国家：
美国
起止时间：
2016-08-01 至 2021-07-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/9171180
关键词：
Address Antigens Apoptosis Area Arthritis Autoimmune Diseases Autoimmune Process Biological Assay Biology Calibration Cell Cycle Cell Proliferation Cell Separation Cell surface Cells Cellular biology Child Childhood Clustered Regularly Interspaced Short Palindromic Repeats Communities Data Data Analyses Data Storage and Retrieval Dendritic Cells Detection Development Disease Equipment Experimental Designs Flow Cytometry Functional disorder Gene Expression Gene Expression Profiling Genetic Goals Health Services Accessibility Human Resources Image Cytometry Immune Immune response Immune system Immunophenotyping Inflammatory Joints Lasers Liquid substance Lymphocyte Maintenance Methodology Pathologic Phenotype Ploidies Population Procedures Process Quality Control RNA Reporter Research Research Personnel Resources Rheumatism Sampling Services Signal Transduction Sorting - Cell Movement Staining method Stains Synovial Fluid Systemic Lupus Erythematosus T-Lymphocyte Technical Expertise Techniques Technology Tissues Training autoinflammatory base cell type cost cytokine data management genome editing instrument macrophage monocyte novel novel therapeutic intervention release of sequestered calcium ion into cytoplasm single cell analysis voltage

项目摘要

Several cell types are involved in inflammatory processes in rheumatic diseases. Understanding the pathobiology of these diseases often requires dissecting out contributions of specific cell subpopulations. The SCPC (Single Cell Phenotyping Core) will provide technical expertise, assistance and equipment at a reasonable cost to investigators in the Research Base of the Cincinnati Rheumatic Disease Core Center (CRDCC) whose studies require single cell analysis. The SCPC will provide training in the proper use and interpretation of a number of immunophenotyping methodologies, including flow cytometry and single cell gene expression. Expertise and assistance will be provided by Core personnel in the following areas: · Cell sorting · Cell surface phenotyping · Intracellular cytokine detection · Gene expression reporter analysis · Cell proliferation analysis · Cell cycle and DNA content analysis · Apoptosis analysis · Intracellular calcium mobilization analysis · Tetramer staining of antigen-specific T cells · RNA detection by flow cytometry · Cell signalling analysis · CRISPR genome-edited cell identification and sorting · Imaging cytometry · Single cell manipulation and gene expression analysis In addition, the SCPC will facilitate acquisition, analysis and storage of immunophenotyping data, maintain standards and quality control for immunophenotyping procedures, and assist in the application and development of new techniques.

几种细胞类型参与风湿病中的炎症过程。了解这些疾病的病理生物学通常需要解剖特定细胞亚群的贡献。 SCPC（单细胞表型核心）将在A中提供技术专业知识，帮助和设备辛辛那提风湿病核心中心研究基础的研究者的合理成本（CRDCC）的研究需要单细胞分析。 SCPC将提供适当使用的培训和解释多种免疫打型方法，包括流式细胞术和单细胞基因表达。核心人员将在以下领域提供专业知识和协助： ·细胞分类细胞表面表型 ·细胞内细胞因子检测基因表达报告基因分析细胞增殖分析细胞周期和DNA含量分析 ·凋亡分析细胞内钙动员分析 ·抗原特异性T细胞的四聚体染色通过流式细胞仪检测RNA 细胞信号分析 CRISPR基因组编辑的细胞识别和分类 ·成像细胞仪单细胞操纵和基因表达分析此外，SCPC将有助于获取，分析和存储免疫表型数据，维护免疫型程序的标准和质量控制，并协助申请和开发新技术。