Core 3 - Integrative Cell Phenotyping and Morphology Core

核心 3 - 综合细胞表型和形态学核心

基本信息

批准号：
8381260
负责人：
SHERRY L THORNTON
金额：
$ 15.93万
依托单位：
CINCINNATI CHILDRENS HOSP MED CTR
依托单位国家：
美国
项目类别：
财政年份：
资助国家：
美国
起止时间：
至
项目状态：
未结题

来源：
https://reporter.nih.gov/project-details/8381260
关键词：
Address Antigens Apoptosis Area Arthritis Autoimmune Diseases Autoimmune Process Biological Assay Biology Calibration Cell Cycle Cell Proliferation Cell Separation Cell surface Cells Cellular biology Child Childhood Data Data Analyses Data Storage and Retrieval Dendritic Cells Development Disease Equipment Experimental Designs Flow Cytometry Functional disorder Gene Expression Goals Histology Human Resources Image Immune Immune response Immune system Immunohistochemistry Immunophenotyping In Situ Hybridization Inflammatory Joints Lasers Lead Liquid substance Lymphocyte Maintenance Methodology Morphology Pathologic Pathology Phenotype Ploidies Population Procedures Process Quality Control Research Research Personnel Rheumatism Sampling Services Synovial Fluid Systemic Lupus Erythematosus T-Lymphocyte Technical Expertise Techniques Technology Tissues Training base cell type cost cytokine data management instrument macrophage monocyte novel therapeutic intervention release of sequestered calcium ion into cytoplasm single cell analysis voltage

项目摘要

Several cell types are involved in inflammatory processes in rheumatic diseases. Understanding the pathobiology of these diseases often requires dissecting out contributions of spedfic cell subpopulations. The Integrative Cell Phenotyping and Morphology Core (ICPMC) will provide technical expertise, assistance and equipment at a reasonable cost to investigators in the Research Base of the Cindnnati Rheumatic Disease Core Center (CRDCC) whose studies require single cell analysis. The ICPMC will provide training in the proper use and interpretation of a number of immunophenotyping methodologies, including flow cytometry, immunohistochemistry, in situ hybridization cind histology. Expertise and assistance will be provided by Core personnel in the following areas: * Cell surface phenotype analysis (flow-based and pathology-based) * Morphological cell phenotype analysis (flow-based and pathology-based) * Intracellular cytokine analysis (flow-based, and pathology-based) * Cell proliferation analysis (flow-based) * Cell cycle and DNA index analysis (flow-based) * Cell apoptosis analysis (flow-based and pathology-based) * Gene expression analysis (flow-based and pathology-based) * Calcium flux analysis (flow-based) * Cell sorting (flow-based) * Antigen-specific T cell analysis (flow-based) In addition, the ICPMC will facilitate acquisition, analysis and storage of immunophenotyping data, maintain standards and quality control for immunophenotyping procedures, and assist in the application and development of new techruques.

几种细胞类型参与风湿病中的炎症过程。了解这些疾病的病理生物学通常需要解剖Spedfic细胞亚群的贡献。综合细胞表型和形态核心（ICPMC）将在Cindnnati风湿病核心中心（CRDCC）的研究基础上以合理的成本提供技术专长，援助和设备，其研究需要单细胞分析。 ICPMC将提供适当使用和解释多个多个的培训免疫表型方法，包括流式细胞术，免疫组织化学，原位杂交CIND组织学。核心人员将在以下领域提供专业知识和协助： *细胞表面表型分析（基于流和病理） *形态细胞表型分析（基于流和病理） *细胞内细胞因子分析（基于流量和基于病理） *细胞增殖分析（基于流） *细胞周期和DNA指数分析（基于流） *细胞凋亡分析（基于流和病理） *基因表达分析（基于流和病理） *钙通量分析（基于流） *细胞分类（基于流） *抗原特异性T细胞分析（基于流）此外，ICPMC将促进免疫表型数据的获取，分析和存储，维护免疫表型程序的标准和质量控制，并协助应用和开发新的Techruques。