Human Skin Disease Resource-based Center

人类皮肤病资源中心

基本信息

批准号：
10615211
负责人：
Rachael Ann Clark
金额：
$ 88.28万
依托单位：
BRIGHAM AND WOMEN'S HOSPITAL
依托单位国家：
美国
项目类别：
财政年份：
2016
资助国家：
美国
起止时间：
2016-07-19 至 2026-04-30
项目状态：
未结题

来源：
https://reporter.nih.gov/project-details/10615211
关键词：
ATAC-seq Acceleration Address Advisory Committees Animal Model Animals Antibodies Biological Biopsy Blood Cells Clinical Trials Color Communication Communities Consent Cryopreservation Cytometry DNA Library Diagnosis Disease Eligibility Determination Environment Flow Cytometry Formalin Frozen Sections Funding Gene Expression Genetic Fingerprintings Genotype Goals Human Human Biology Image Immune system Immunodeficient Mouse Individual Institution International Knowledge Leadership Malignant Neoplasms Medicine Mentorship Modeling Mus Paraffin Embedding Pathologic Pathology Patients Physicians Plasma Population Positioning Attribute Protein Analysis Proteins Protocols documentation RNA Research Research Personnel Research Project Grants Resources Sampling Science Scientist Serum Services Shipping Skin Specimen System T cell receptor repertoire sequencing T-Cell Receptor Genes Talents Techniques Technology Time Tissue Embedding Tissues Training Underrepresented Populations Vaccination Woman Work biobank cellular imaging cost digital empowerment human tissue insight member methicillin resistant Staphylococcus aureus mouse model nano-string new technology next generation outreach people of color protein expression recruit single-cell RNA sequencing skin disorder spectrograph symposium therapy development tool translational scientist

项目摘要

Project Summary/Abstract: Overall Component Mouse models have provided many biologic insights and remain the most popular system in which to conduct skin disease research. However. there are significant differences between the skin and immune systems of mice and humans and these differences are incompletely characterized, making it difficult to know if observations made in mice will hold true in humans. Research carried out on human cells and tissues can address this knowledge gap. Human biobanks and powerful new analytic techniques have become available that make high-quality human skin disease research accessible. The goals of this Center are to accelerate human skin disease research by providing researchers at any institution with access to human specimens and cutting edge analytic techniques and to bring new diverse investigators into the field of human skin disease research. We include 45 research projects from investigators who wish to utilize Center services; however, any researcher wishing to carry out human skin disease research is a potential member of the research community. The Center is composed of an Administrative Core and three Resource Cores. The Administrative Core manages and oversees all activities of the Center and administers Diversity and Outreach activities, including funding for diverse investigators at multiple stages of training and the biennial International Conference on Human Skin Disease. The Human Tissues Biobank Core provides access to over 113,000 highly characterized consented patients, over 1.5 million banked pathologic specimens, both searchable by diagnosis, as well as to fresh human skin, purified cell populations from human skin and immunodeficient mice grafted with human skin and blood. The Single Cell and Immunoanalysis Core provides access to single cell RNA and ATAC sequencing, flow cytometry-based single cell imaging, mass cytometry by time of flight (CyTOF) and high throughput TCR sequencing. The Next Generation Tissue Analysis & Imaging Core provides access to six color tyramide amplification based immunostaining combined with spectral imaging and automated cell analysis, NanoString RNA and DNA profiling and Digital Spatial Profiling, a state-of-the-art technique that that can profile expression of 10-1000s of protein or RNA targets in FFPE and frozen sections. In summary, the Center provides access to biobanks and cutting-edge human analytic techniques that enable translational researchers to carry out high quality human skin disease research.

项目摘要/摘要：总体组成部分小鼠模型提供了许多生物学见解，并且仍然是最受欢迎的系统皮肤病研究。然而。皮肤和免疫系统之间存在显着差异小鼠和人类之间的差异尚未完全表征，因此很难知道是否在小鼠身上进行的观察也适用于人类。对人体细胞和组织进行的研究可以解决这一知识差距。人类生物库和强大的新分析技术已经可用使高质量的人类皮肤病研究成为可能。该中心的目标是加速人类皮肤病研究，为任何机构的研究人员提供人体样本和尖端的分析技术，并将新的多元化研究人员带入人类皮肤病领域研究。我们纳入了 45 个来自希望利用中心服务的研究人员的研究项目；然而，任何希望进行人类皮肤病研究的研究人员是该研究的潜在成员社区。该中心由一个行政核心和三个资源核心组成。行政部门核心管理和监督中心的所有活动，并管理多元化和外展活动，包括为不同研究人员在培训的多个阶段和两年一次的国际研究提供资金人类皮肤病会议。人体组织生物库核心提供超过 113,000 高度特征化的同意患者，超过 150 万份储存的病理标本，均可通过诊断，以及新鲜的人类皮肤、来自人类皮肤和免疫缺陷小鼠的纯化细胞群移植了人类的皮肤和血液。单细胞和免疫分析核心提供了对单细胞的访问 RNA 和 ATAC 测序、基于流式细胞术的单细胞成像、飞行时间质谱流式细胞术 (CyTOF) 和高通量 TCR 测序。下一代组织分析和成像核心提供基于六色酪酰胺扩增的免疫染色结合光谱成像和自动化细胞分析、NanoString RNA 和 DNA 分析以及数字空间分析，这是最先进的该技术可以分析 FFPE 和冷冻切片中 10-1000 个蛋白质或 RNA 靶标的表达。总之，该中心提供生物库和尖端人体分析技术，使转化研究人员开展高质量的人类皮肤病研究。

项目成果

期刊论文数量（27）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

Duality at the gate: Skin dendritic cells as mediators of vaccine immunity and tolerance.

门口的二元性：皮肤树突状细胞作为疫苗免疫和耐受性的介质。

DOI：
发表时间：
2016
期刊：
影响因子：
4.8
作者：
Nirschl, Christopher J;Anandasabapathy, Niroshana
通讯作者：
Anandasabapathy, Niroshana

Evaluation of Low-Dose, Low-Frequency Oral Psoralen-UV-A Treatment With or Without Maintenance on Early-Stage Mycosis Fungoides: A Randomized Clinical Trial.

低剂量、低频口服补骨脂素-UV-A 治疗联合或不联合维持治疗早期蕈样肉芽肿的评估：一项随机临床试验。

DOI：
发表时间：
2019
期刊：
影响因子：
10.9
作者：
Vieyra;Fink;Porkert, Stefanie;Lang, Roland;Pöchlauer, Sophie;Ratzinger, Gudrun;Tanew, Adrian;Selhofer, Sylvia;Paul;Hofer, Angelika;Gruber;Legat, Franz;Patra, Vijaykumar;Quehen
通讯作者：
Quehen

CCR8 is a new therapeutic target in cutaneous T-cell lymphomas.

CCR8是皮肤T细胞淋巴瘤的新治疗靶点。

DOI：
发表时间：
2022-06-14
期刊：
影响因子：
7.5
作者：
Giustiniani, Jérôme;Dobos, Gabor;Moins;Eustaquio, Tiago;Battistella, Maxime;Ortonne, Nicolas;Ram;Bouaziz, Jean;Marie;Mourah, Samia;Bagot, Martine;Kupper, Thomas S;Clark, Rachael A;Bensuss
通讯作者：
Bensuss

The origin of DCs and capacity for immunologic tolerance in central and peripheral tissues.

DC 的起源以及中枢和外周组织的免疫耐受能力。

DOI：
发表时间：
2017
期刊：
影响因子：
0
作者：
Devi, K Sanjana P;Anandasabapathy, Niroshana
通讯作者：
Anandasabapathy, Niroshana

Pathogenic mycobacteria achieve cellular persistence by inhibiting the Niemann-Pick Type C disease cellular pathway.

致病性分枝杆菌通过抑制尼曼-皮克 C 型疾病细胞途径来实现细胞持久性。

DOI：
发表时间：
2016-11-18
期刊：
影响因子：
0
作者：
Fineran, Paul;Lloyd;Lack, Nathan A;Platt, Nick;Davis, Lianne C;Morgan, Anthony J;Höglinger, Doris;Tatituri, Raju Venkata V;Clark, Simon;Williams, Ian M;Tynan, Patricia;Al Eisa, Nada;Nazarova, Evgeniya;Williams, Ann;Galione, Anton
通讯作者：
Galione, Anton