HIMC Core

HIMC核心

• 批准号: 9041502
• 负责人: Holden T. Maecker
• 金额: $ 12.11万
• 依托单位: STANFORD UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2003
• 资助国家: 美国
• 起止时间: 2003-09-30 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/9041502
• 关键词: Algorithms; Antibodies; Archives; Area; Biological Assay; Blood; Clinical; Clinical Data; Clinical Research; Collaborations; Communication; Complex; Computer software; Cytomegalovirus; Cytometry; DNA; Data; Data Aggregation; Data Analyses; Data Set; Databases; Decision Trees; Ensure; Environment; Enzyme-Linked Immunosorbent Assay; Generations; Hemagglutinin; Human; Human Herpesvirus 4; Human Resources; Immune; Immunologic Monitoring; Immunology; Individual; Infection; Instruction; Label; Machine Learning; Maintenance; Maps; Memory; Mining; Natural Immunity; Peripheral Blood Mononuclear Cell; Process; RNA; Research Personnel; Retrieval; Robotics; Running; ST14 gene; Sampling; Sampling Studies; Serum; Services; Specimen; System; Testing; Training; Translational Research; Vaccination; Work; adaptive immunity; biobank; data integration; experience; relational database; tool

By combining immune monitoring on multiple platforms in a single center, the Human Immune Monitoring Center (HIMC) has created an ideal environment for all aspects of human immunology studies, from sample processing and banking, to immune assessment assays, and data analysis and integration. Of particular relevance to this U19, we have an efficient system for high capacity sample banking and retrieval, working closely with the Clinical and Translational Research Unit (CTRU) and clinical coordinators to ensure accurate labeling, sample tracking, and optimal storage and retrieval for assays. We have also standardized both basic and advanced immune monitoring platforms, from simple ELISA assays for determination of CMV and EBV antibody titers, to complex CyTOF mass cytometry assays. In our first Specific Aim, we will process and bank PBMC, serum, and RNA for clinical specimens, in collaboration with the CTRU and Clinical Core. In our second Specific Aim, we will offer CMV and EBV antibody testing and hemagglutinin inhibition assays, provide DNA and RNA extraction services, and run CyTOF assays for CCHI projects. We have highly trained personnel in all of these areas to support the generation of optimized and standardized data. Finally, in our third Specific Aim, we will offer data integration services through our online relational database, Stanford Data Miner (SDM). With newly developed capabilities, SDM will allow integration of data across the above assays and with relevant clinical variables. It will also be able to mine these integrated data sets using on-board machine learning tools such as decision trees and association rules mining.

通过在单个中心的多个平台上结合免疫监测，人类免疫监测 中心（HIMC）从样本中为人类免疫学研究的各个方面创造了一个理想的环境 处理和银行业务，免疫评估测定以及数据分析和集成。特别 与此U19相关，我们有一个有效的系统用于高容量的样本银行和检索 与临床和转化研究单元（CTRU）和临床协调员紧密联系，以确保准确 标记，样品跟踪以及测定的最佳存储和检索。我们还标准化了 基本和先进的免疫监控平台，从简单的ELISA分析来确定CMV和 EBV抗体滴度，以进行复杂的质量细胞术测定。在我们的第一个特定目标中，我们将处理 以及与CTRU和临床核心合作的临床标本的银行PBMC，血清和RNA。 在我们的第二个特定目标中，我们将提供CMV和EBV抗体测试以及血凝素抑制分析， 提供DNA和RNA提取服务，并为CCHI项目运行Cytof测定法。我们有很高的 在所有这些领域的训练有素的人员支持生成优化和标准化的数据。最后， 在我们的第三个特定目标中，我们将通过在线关系数据库提供数据集成服务， 斯坦福数据矿工（SDM）。有了新开发的功能，SDM将允许整合数据 上面的测定和相关的临床变量。它也将能够挖掘这些集成的数据集 使用板载机器学习工具，例如决策树和关联规则挖掘。