Diabetes Immune Monitoring Core

糖尿病免疫监测核心

基本信息

批准号：
10669014
负责人：
Holden T. Maecker
金额：
$ 19.7万
依托单位：
STANFORD UNIVERSITY
依托单位国家：
美国
项目类别：
财政年份：
2017
资助国家：
美国
起止时间：
2017-09-15 至 2027-06-30
项目状态：
未结题

来源：
https://reporter.nih.gov/project-details/10669014
关键词：
Abbreviations Adult Alpha Cell Antigens Antithymoglobulin Archives Autoantibodies Award Basic Science Beta Cell Biological Assay Biology Blood Blood Cells Blood specimen Bone Marrow California Cell Death Cell Lineage Cell Therapy Cells Cellular Metabolic Process Cellular biology Child Health Clinical Clinical Research Clinical Trials Clinics and Hospitals Collaborations Communities Cryopreservation Data Development Diabetes Mellitus Dimensions Donor person Electronic Health Record Ensure Epigenetic Process Etiology Evaluation Evolution Explosion Feedback Flow Cytometry Frequencies Functional disorder Funding Genetic Genomics Growth Healthcare Hematopoietic stem cells Human Human Resources Immune Immune Tolerance Immune response Immunity Immunobiology Immunologic Factors Immunologic Monitoring Immunology Immunology procedure Immunotherapy Infection Inflammation Inflammatory Insulin-Dependent Diabetes Mellitus Investigation Islets of Langerhans Islets of Langerhans Transplantation Kidney Leukocytes Link Living Donors Methods Microspheres Mixed Lymphocyte Culture Test Modernization Molecular Monitor Myeloid Cells Non-Insulin-Dependent Diabetes Mellitus Pancreas Pathologic Pathology Patient Care Patient Monitoring Patient-Focused Outcomes Patients Phenotype Physiological Plasma Pre-Clinical Model Process Reagent Regulatory T-Lymphocyte Research Research Institute Research Personnel Resources Running Sampling Serum Services Signal Transduction Spleen Standardization Study Subject T-Lymphocyte Subsets T-cell receptor repertoire Technology Testing Therapy Clinical Trials Time Tissue Procurements Tissue Sample Tissues Training Translational Research Transplantation Transplantation Tolerance Universities Work base biobank cell killing chemokine chimeric antigen receptor chimeric antigen receptor T cells clinical center cytokine diabetes risk exome sequencing granulocyte hematopoietic cell transplantation high dimensionality human leukocyte antigen testing human tissue immunogenic improved instrumentation interest investigator training islet lymph nodes lymphoid irradiation medical schools member next generation sequencing patient stratification pediatric patients peripheral blood programs research study response success tool transcriptome sequencing

项目摘要

DIABETES IMMUNE MONITORING CORE (DIMC): PROJECT SUMMARY/ABSTRACT The specialized expertise of the Diabetes Immune Monitoring Core (DIMC) successfully provides SDRC investigators with the capacity to perform modern molecular, cellular and functional studies of human immune cells in type 1 (T1D), type 2 (T2D) and type 3c (T3cD) diabetes. Many SDRC members perform collaborative studies of immuno-biology in physiological or pathological settings, including transplant tolerance, inflammation, cell signaling, immune cell metabolism, immunogenic vs non-immunogenic cell death, genetics, epigenetics, islet immunology and immune therapy. A key feature of the DIMC is our direct focus on human immunology with the assessment of leukocytes in blood and tissues. An essential component is the provision of validated and standardized high dimensional instrumentation, reagents and assays to probe B, NK, T, myeloid and granulocytic cell lineages in blood and tissue from patients with or at-risk for diabetes. These robust tools have increased the number of SDRC investigators studying human diabetes at Stanford. In addition, the DIMC has evolved to meet the emerging interests of SDRC investigators by developing human tissue procurement for immuno-biology studies related to diabetes, including archiving of fractionated blood cells and serum from subjects with new-onset T1D, and isolation and banking of matched bone marrow/hematopoietic stem cells in coordination with the SDRC Islet Research Core. These activities are not provided by any other research core to diabetes researchers at Stanford. The DIMC’s current support also enhances the emerging Stanford Pancreatic Islet Replacement and Immune Tolerance (SPIRIT) human islet transplantation program for Stanford Health Care (SHC) patients with T3cD and eventually T1D. The DIMC also provides training for investigators in specialized methods of human Immunology such as CyTOF, high dimensional flow cytometry, cytokine/chemokine microbead arrays, mixed lymphocyte reactions, T cell receptor repertoire analysis via RNASeq and monitoring of B, T and APC responses to ß cells and their antigens. DIMC personnel will continue to work closely with SDRC investigators to build efficient experimental strategies tailored to their needs. This support will help investigators develop the new clinical programs at SHC. Integration of DIMC efforts with other SDRC research cores will continue to enrich and enhance studies of human immune cells relevant to diabetes. The DIMC evolves to serve the SDRC community by continuously developing new experimental capacity to match an explosion in sophisticated technologies. The DIMC will also serve SDRC members at the Universities of California at Berkeley or at Davis, including those supported through the proposed Regional Pilot & Feasibility Award expansion. Based on growth of the SDRC membership, evolution of exciting new services, increasing membership demand for human immune and hematopoietic stem cell studies, and the increased research focus on immune and transplantation biology, we anticipate increased demand and use of specialized or unique DIMC services in the coming years.

糖尿病免疫监测核心 (DIMC)：项目摘要/摘要糖尿病免疫监测核心 (DIMC) 的专业知识成功地为 SDRC 具有对人体免疫进行现代分子、细胞和功能研究能力的研究人员许多 SDRC 成员合作研究 1 型 (T1D)、2 型 (T2D) 和 3c 型 (T3cD) 糖尿病的细胞。生理或病理环境下的免疫生物学研究，包括移植耐受性，炎症、细胞信号传导、免疫细胞代谢、免疫原性与非免疫原性细胞死亡、遗传学、表观遗传学、胰岛免疫学和免疫治疗 DIMC 的一个关键特点是我们直接关注人类。评估血液和组织中的白细胞的免疫学的一个重要组成部分是提供。经验证和标准化的高维仪器、试剂和检测方法，用于探测 B、NK、T、糖尿病患者或有糖尿病风险的患者血液和组织中的骨髓和粒细胞谱系。强大的工具增加了斯坦福大学研究人类糖尿病的 SDRC 研究人员的数量。此外，DIMC 已经发展到通过开发人力来满足 SDRC 调查人员的新兴趣。与糖尿病相关的免疫生物学研究的组织采购，包括分级血液的存档来自新发 T1D 受试者的细胞和血清，以及匹配骨的分离和储存与 SDRC 胰岛研究核心协调的骨髓/造血干细胞这些活动不是。 DIMC 当前的支持也由任何其他研究核心向斯坦福大学的糖尿病研究人员提供。增强新兴的斯坦福胰岛替代和免疫耐受 (SPIRIT) 人类胰岛斯坦福医疗保健 (SHC) 的 T3cD 患者和最终 T1D 患者的移植计划 DIMC。还为研究人员提供人类免疫学专业方法的培训，例如 CyTOF、高三维流式细胞术、细胞因子/趋化因子微珠阵列、混合淋巴细胞反应、T 细胞通过 RNASeq 进行受体库分析并监测 B、T 和 APC 对 ß 细胞及其反应 DIMC 人员将继续与 SDRC 研究人员密切合作，建立高效的实验。根据他们的需求制定策略，这种支持将帮助研究人员开发新的临床项目。 SHC。DIMC 工作与 SDRC 其他研究核心的整合将继续丰富和加强研究。 DIMC 不断发展，为 SDRC 社区服务。 DIMC 还将开发新的实验能力以适应尖端技术的爆炸式增长。为加州大学伯克利分校或戴维斯分校的 SDRC 成员提供服务，包括那些受支持的成员根据 SDRC 的发展，通过拟议的区域试点和可行性奖扩展。会员资格、令人兴奋的新服务的发展、对人类免疫和免疫系统的会员需求不断增加造血干细胞研究，以及对免疫和移植生物学的研究日益关注，我们预计未来几年对专业或独特 DIMC 服务的需求和使用将会增加。