Human Immune Monitoring Core

人体免疫监测核心

• 批准号: 10491677
• 负责人: Holden T. Maecker
• 金额: $ 110.77万
• 依托单位: STANFORD UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2021
• 资助国家: 美国
• 起止时间: 2021-09-21 至 2026-08-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10491677
• 关键词: ATAC-seq; Adopted; Aging; Antibodies; Antigens; Back; Biological Assay; Blood; Cells; Clinical; Computer software; Custom; Cytometry; DNA; Data; Data Aggregation; Data Analyses; Databases; Deposition; Equipment and supply inventories; Flow Cytometry; Gene Expression; Gene Proteins; Generations; Goals; Head; Hemagglutinin; Human; Immune; Immunologic Monitoring; Immunology; Immunology procedure; Immunophenotyping; Infrastructure; Link; Maps; Measures; Methylation; Monitor; Peer Review; Peripheral Blood Mononuclear Cell; Phenotype; Procedures; Process; Production; Publications; Quality Control; RNA; Reporting; Research; Research Personnel; Resolution; Retrieval; Sampling; Sampling Studies; Serum; Specimen; Standardization; System; T cell receptor repertoire sequencing; Technology; Time; Universities; Whole Blood; base; biobank; cohort; cytokine; data management; data reuse; experience; flu; glycosylation; high dimensionality; human leukocyte antigen testing; improved; metabolomics; microbiome; organizational structure; programs; sample archive; usability; vaccine response; web portal

Summary The Immune Monitoring and Data Management Core (IMDMC) will be responsible for sample processing and banking, comprehensive and standardized immune monitoring of those samples, and archiving and organization of data generated. Sample processing and banking will be performed through the Stanford Biobank, using well- developed systems and standard operating procedures (SOPs). The Stanford Human Immune Monitoring Center (HIMC) will perform highly comprehensive assays of immune phenotype and function on both pre-existing and newly collected samples. These will include standardized O-Link cytokine arrays, CyTOF immunophenotyping, phosphosignaling flow cytometry, HLA typing, whole blood gene expression, and hemagglutinin inhibition (HAI) assays; as well as new data types including microbiome, metabolomics, methylation, TCR sequencing, ATACseq on sorted cells, single cell gene expression via BD Rhapsody, flu antigen arrays, and antibody glycosylation analysis. Quality control measures will be performed on all assays, and data uploaded and organized via an existing online database, Stanford Data Miner (SDM). SDM will allow integration with existing data, across assays, and with clinical and demographic data. It will facilitate access by all associated investigators, as well as extraction for upload to the public ImmPort database. Relevance: The IMDMC will provide access to a suite of comprehensive, state-of-the-art immune assays, which have already been applied to many existing samples. This unmatched set of samples, technologies, and aggregated data will facilitate the discovery of new metrics and mechanisms of immune aging, vaccine responsiveness, and many other avenues of research.

概括 免疫监控和数据管理核心（IMDMC）将负责样本处理和 银行业务，全面和标准化的免疫监控，并归档和组织 生成的数据。样本处理和银行业务将通过斯坦福生物库来进行，使用良好 开发的系统和标准操作程序（SOP）。斯坦福人免疫监测中心 （HIMC）将对先前存在和 新收集的样品。这些将包括标准化的O-link细胞因子阵列，细胞cytof免疫表型， 磷酸化流式细胞术，HLA打字，全血基因表达和血凝素抑制（HAI）（HAI） 测定；以及包括微生物组，代谢组学，甲基化，TCR测序，ATACSEQ在内的新数据类型 在排序的细胞上，通过BD狂想曲，流感抗原阵列和抗体糖基化的单细胞基因表达 分析。质量控制措施将对所有测定法执行，并通过 现有的在线数据库，斯坦福数据矿工（SDM）。 SDM将允许与现有数据集成， 测定，以及临床和人口统计数据。这也将促进所有相关研究人员的访问 作为上传到公共Inmport数据库的提取。 相关性：IMDMC将提供一套全面的，最先进的免疫测定法 已经应用于许多现有样本。这套无与伦比的样本，技术和 汇总数据将有助于发现免疫老化，疫苗的新指标和机制 响应能力以及许多其他研究途径。