ShEEP Request for Zeiss LSM880 with Airyscan

ShEEP 请求使用 Airyscan 的 Zeiss LSM880

• 批准号: 9211461
• 负责人: Ann C. McKee
• 金额: --
• 依托单位: VA BOSTON HEALTH CARE SYSTEM
• 依托单位国家: 美国
• 财政年份: 2016
• 资助国家: 美国
• 起止时间: 2016-05-01 至 2016-09-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/9211461
• 关键词: Alzheimer' s Disease; Biological; Boston; Brain imaging; Brain region; Cell physiology; Collection; Confocal Microscopy; Core Facility; Data; Development; Dimensions; Discipline; Excision; Fluorescence; Funding; Healthcare Systems; Housing; Human; Image; Individual; Jamaica; Label; Microscope; Neurodegenerative Disorders; Neurons; Neurosciences; Photobleaching; Proteins; Reporter; Research; Research Personnel; Resolution; Signal Transduction; Specimen; Speed; System; Technology; Thick; Three-Dimensional Imaging; Time; Tissue Sample; Tissues; Transgenic Animals; Traumatic Brain Injury; base; brain tissue; connectome; design; detector; human tissue; improved; instrument; new technology; optical spectra; optogenetics; quantitative imaging; spectrograph

This proposal describes a request for funds to purchase a Zeiss LSM880 Airyscan multichannel spectral imaging microscope with 32-channel detector and clarity objective. This is a versatile, integrated state-of-the-art super-resolution microscope system that will be used by more than ten funded VA investigators at the VA Boston Healthcare System (VABHS) to further research in the neurosciences and other disciplines. The present application describes our need for this instrument, its technical specifications, examples of how research can be furthered at VABHS through the use of this instrument, and a plan for its administration. Recent technical developments such as optogenetics, the use of cleared tissues, and transgenic animals expressing fluorescent protein- based reporters for cell function have enabled unprecedented possibilities in the neurosciences and other biological disciplines. The Airyscan super-resolution microscope will allow investigators at VABHS to take advantage of these new technologies. The ability to clearly resolve individual neurons and their connections in cleared tissues will allow unprecedented visualization of brain networks. This instrument will be of particular importance in moving discovery into cellular mechanisms of traumatic brain injury and Alzheimer’s disease to the next level. In spite of the clear need, there is currently no such instrument at VABHS.

该提案描述了购买Zeiss LSM880 Airyscan Multichannel的资金请求 具有32通道检测器和清晰度的光谱成像显微镜。这是一个通用的， 整合的最先进的超分辨率显微镜系统将被十多个使用 VA波士顿医疗保健系统（VABHS）资助的VA调查人员，以进一步研究 神经科学和其他学科。本应用程序描述了我们对此工具的需求， 它的技术规范，如何通过使用在VABHS进行研究的示例 该工具和管理计划。最近的技术发展，例如 光遗传学，清除组织的使用以及表达荧光蛋白的转基因动物 基于细胞功能的记者已经实现了神经科学的前所未有的可能性 其他生物学学科。 Airyscan超分辨率显微镜将允许研究人员 VABHS利用这些新技术。明确解决个人的能力 神经元及其在清除组织中的连接将允许大脑的前所未有的可视化 网络。该仪器在将发现转移到蜂窝中特别重要 创伤性脑损伤和阿尔茨海默氏病的机制达到了新的水平。尽管很清楚 需要，目前在Vabhs没有这样的乐器。