Sample Engineering Core

工程核心示例

• 批准号: 9118857
• 负责人: Frederic Zenhausern
• 金额: $ 53.33万
• 依托单位: COLUMBIA UNIVERSITY HEALTH SCIENCES
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/9118857
• 关键词: 3D Print; Area; Automation; Biological Assay; Blood; Blood Volume; Blood capillaries; Blood specimen; Cell Culture Techniques; Collection; Construction Materials; Culture Media; Cytogenetics; Development; Devices; Engineering; Ensure; Fingers; Gel; Gene Expression Profiling; Individual; Industry; Laboratories; Link; Liquid substance; Mass Spectrum Analysis; Methods; Microfluidics; Micronucleus Tests; Mitogens; Needles; Paper; Performance; Process; Protocols documentation; Radiation; Reagent; Sampling; Shipping; Ships; System; Systems Development; Techniques; Technology; Temperature; Testing; Time; Transportation; Tube; Universities; Vacuum; Validation; absorption; assay development; base; biodosimetry; biomaterial compatibility; capillary; design; high throughput analysis; instrument; light weight; manufacturing process; metabolomics; minimally invasive; pressure; prototype; research clinical testing; sample collection; seal; small molecule; wasting

The function of the Sample Engineering Core is to advance pre-analysis strategies and methods including sample collection, in-field preprocessing, and transportation of samples. The Core will leverage expertise in microfluidic design, rapid prototyping, manufacturing process engineering, and biological assay development to create devices and protocols for each of the three Projects. The Core will perform tasks in close coordination with the three Projects to ensure accurate endpoints when linking to the automated high-throughput analysis instruments, which are becoming increasingly available in university, industry, and clinical testing laboratories. The first aim of the Sample Engineering Core is to develop a semi-automated device for collection and pre- processing of blood samples, applicable to all of the Projects. Prototypes of the device will be manufactured using advanced 3D printing technology. The second aim is to develop in-field pre-processing technologies to stabilize blood samples for transportation while concurrently beginning the analysis process. A method of stabilizing and shipping samples for use with Project 1 and 2 endpoints will utilize vacuum-sealed tubes preloaded with reagents. A paper-based stabilization card will be developed to use with Project 3 endpoints. The final aim is to develop an inexpensive, lightweight, battery-powered shipping container capable of precise thermal control during transportation to centralized analysis facilities. This development will allow the micronucleus assay culture for Project 1 to begin immediately following sample acquisition, therefore utilizing shipping time which was formerly wasted. The end result will be faster times to final biodosimetric results.

样本工程核心的功能是提出预先分析策略和方法，包括 样品收集，场地预处理和样品的运输。核心将利用专业知识 微流体设计，快速原型制作，制造过程工程和生物学测定开发 为三个项目中的每个项目创建设备和协议。核心将在密切协调中执行任务 通过三个项目，可以在链接到自动高通量分析时确保准确的终点 在大学，工业和临床测试实验室中越来越多的仪器。 样本工程核心的第一个目的是开发一种半自动化设备，以收集和预先 血液样本的处理，适用于所有项目。设备的原型将制造 使用高级3D打印技术。 第二个目的是开发现场预处理技术以稳定运输的血液样本 同时开始分析过程。一种稳定和运送样品的方法 项目1和2端点将利用带有试剂预装的真空密封管。基于纸的 将开发稳定卡以与项目3端点一起使用。 最终目的是开发一个廉价，轻巧，电池供电的运输容器，能够精确 运输到集中分析设施的热控制。这种发展将允许 项目1的微核测定培养物在样本采集后立即开始，因此利用 以前浪费的运输时间。最终结果将是最终生物标准结果的速度更快。