Sample Engineering Core

工程核心示例

• 批准号: 9321356
• 负责人: Frederic Zenhausern
• 金额: $ 30.73万
• 依托单位: COLUMBIA UNIVERSITY HEALTH SCIENCES
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/9321356
• 关键词: Alpha Cell; Area; Automation; Biological Assay; Blood; Blood Volume; Blood capillaries; Blood specimen; Cell Culture Techniques; Collection; Construction Materials; Cytogenetics; Development; Devices; Engineering; Ensure; Fingers; Gel; Gene Expression Profiling; Individual; Industry; Laboratories; Link; Liquid substance; Mass Spectrum Analysis; Methods; Microfluidics; Micronucleus Tests; Mitogens; Needles; Paper; Performance; Printing; Process; Protocols documentation; Radiation; Reagent; Sampling; Shipping; Ships; System; Systems Development; Techniques; Technology; Temperature; Testing; Time; Transportation; Tube; Universities; Vacuum; Validation; absorption; assay development; base; biodosimetry; biomaterial compatibility; capillary; design; high throughput analysis; instrument; light weight; manufacturing process; metabolomics; minimally invasive; pressure; prototype; research clinical testing; sample collection; seal; small molecule

The function of the Sample Engineering Core is to advance pre-analysis strategies and methods including sample collection, in-field preprocessing, and transportation of samples. The Core will leverage expertise in microfluidic design, rapid prototyping, manufacturing process engineering, and biological assay development to create devices and protocols for each of the three Projects. The Core will perform tasks in close coordination with the three Projects to ensure accurate endpoints when linking to the automated high-throughput analysis instruments, which are becoming increasingly available in university, industry, and clinical testing laboratories. The first aim of the Sample Engineering Core is to develop a semi-automated device for collection and pre- processing of blood samples, applicable to all of the Projects. Prototypes of the device will be manufactured using advanced 3D printing technology. The second aim is to develop in-field pre-processing technologies to stabilize blood samples for transportation while concurrently beginning the analysis process. A method of stabilizing and shipping samples for use with Project 1 and 2 endpoints will utilize vacuum-sealed tubes preloaded with reagents. A paper-based stabilization card will be developed to use with Project 3 endpoints. The final aim is to develop an inexpensive, lightweight, battery-powered shipping container capable of precise thermal control during transportation to centralized analysis facilities. This development will allow the micronucleus assay culture for Project 1 to begin immediately following sample acquisition, therefore utilizing shipping time which was formerly wasted. The end result will be faster times to final biodosimetric results.

样品工程核心的功能是推进预分析策略和方法，包括 样品采集、现场预处理和样品运输。核心将利用以下方面的专业知识 微流体设计、快速原型制作、制造工艺工程和生物测定开发 为三个项目中的每一个创建设备和协议。核心将密切协调执行任务 与三个项目一起确保链接到自动化高通量分析时准确的终点 大学、工业和临床测试实验室越来越多地使用这些仪器。 样品工程核心的首要目标是开发一种用于收集和预处理的半自动化设备。 血液样本的处理，适用于所有项目。该设备的原型将被制造 采用先进的3D打印技术。 第二个目标是开发现场预处理技术以稳定运输中的血液样本 同时开始分析过程。一种稳定和运输样品以供使用的方法 项目 1 和 2 终点将使用预装试剂的真空密封管。纸质的 将开发稳定卡以与项目 3 端点一起使用。 最终目标是开发一种廉价、轻便、电池供电的集装箱，能够精确地 运输到集中分析设施期间的热控制。这一发展将使 项目 1 的微核测定培养在样品采集后立即开始，因此利用 以前浪费的运输时间。最终结果将更快地获得最终生物剂量测定结果。