Plant photoreceptor CRY2 and Signaling Mechanism

植物光感受器CRY2及其信号机制

基本信息

批准号：
9275106
负责人：
CHENTAO LIN
金额：
$ 1.46万
依托单位：
UNIVERSITY OF CALIFORNIA LOS ANGELES
依托单位国家：
美国
项目类别：
财政年份：
1997
资助国家：
美国
起止时间：
1997-08-01 至 2018-02-28
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/9275106
关键词：
Address Animals Arabidopsis Bacteria Binding Biological Models Biology Circadian Rhythms Code Complex Core Protein DNA Sequence Electrons Elements F Box Domain F-Box Proteins Flowers Funding Gene Expression Genes Genetic Transcription Genetic study Goals Health Human In Vitro Investigation Laboratories Lasers Light Mediating Messenger RNA Methodology MicroRNAs Molecular Mutagenesis Organism Oxidation-Reduction Phosphorylation Photochemistry Photoreceptors Phototransduction Plant Photoreceptors Plants Post-Transcriptional Regulation Post-Translational Regulation Proteins Proteolysis Protons RNA Sequences RNA-Binding Proteins Reaction Regulation Signal Transduction Site Testing Transgenes Triad Acrylic Resin Untranslated Regions Work base biophysical analysis chromophore circadian pacemaker cryptochrome driving force in vivo inhibitor/antagonist light entrainment mRNA Expression mutant novel phyA phytochrome plant growth/development protein complex protein degradation receptor response tool

项目摘要

DESCRIPTION (provided by applicant): How organisms respond to light and how photosensory receptors mediate light responses are basic questions in biology. Our long-term goal is to find the molecular explanation of these questions, using the cryptochrome photoreceptor as a model system. Cryptochromes (CRY) are the blue/UV-A light receptors and/or core components of the circadian oscillator found in all evolutionary lineages including human. My laboratory focuses on the study of plant cryptochromes. In the previous funding periods, we discovered two major CRY2 signal transduction mechanisms: the CIB1 (Cryptochrome-Interacting bHLH 1)-based transcription- regulatory mechanism and SPA1/COP1 (Suppressor of Phytochrome A 1/Constitutive Photomorphogenesis 1)-based proteolysis-regulatory mechanism. More recently, we identified a CIB-interacting protein FOF1 (F-box of Flowering 1) responsible for the blue light-regulated degradation of CIB1; two new blue light-specific CRY2-interacting proteins: PRR5 (Pseudo Response Regulator 5) that is a core protein of the circadian clock, and a novel protein BIC1 (Blue- light Inhibitor of CRYs 1) tha suppresses blue light-dependent phosphorylation, degradation, and activities of CRY1 and CRY2. Based on these discoveries and newly developed tools, we propose to study three key issues of light signal transduction: the photochemical mechanism underlying photoexcitation of the CRY photoreceptor, mechanisms governing the function and regulation of the CRY complexome, and the mechanism underlying a novel coding sequence (CDS)-dependent blue light regulation of CRY2 expression.

描述（由申请人提供）：生物如何对光的反应以及光感受体如何介导光反应是生物学中的基本问题。我们的长期目标是将这些问题的分子解释使用，使用加密色素光感受器作为模型系统。加密色素（Cry）是在包括人类在内的所有进化谱系中发现的昼夜节振荡器的蓝/UV-A光受体和/或核心成分。我的实验室专注于对植物隐性物质的研究。在前面的资金期间，我们发现了两个主要的Cry2信号转导机制：基于CIB1（Cryctodochrome Interacting BHLH 1）基于基于转录的调节机制和SPA1/COP1（植物色素的抑制剂A 1/组成型光塑形1）基于基于蛋白质的蛋白质解析机制。最近，我们鉴定了CIB相互作用的蛋白FOF1（开花的F-box 1）负责CIB1的蓝色光调节降解；两种新的蓝光特异性CRY2相互作用蛋白：PRR5（伪反应调节剂5），该蛋白是昼夜节律时钟的核心蛋白质，而新颖的蛋白BIC1（Crys的蓝光抑制剂1）THA抑制蓝色光依赖性依赖性磷酸化磷酸化，去递观，脱落和crier1和Cry2的活性。基于这些发现和新开发的工具，我们建议研究光信号转导的三个关键问题：Cry光感受器的光激发光激发的光化学机制，管理哭泣复合体功能和调控的机制以及具有新颖的编码序列（CDS）依赖性蓝色光调节的机制。