Plant Photosensory Receptor CRY2 Signaling Mechanism

植物光敏受体 CRY2 信号机制

• 批准号: 6988848
• 负责人: CHENTAO LIN
• 金额: $ 41.72万
• 依托单位: UNIVERSITY OF CALIFORNIA LOS ANGELES
• 依托单位国家: 美国
• 财政年份: 1997
• 资助国家: 美国
• 起止时间: 1997-08-01 至 2009-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6988848
• 关键词: Arabidopsis; biological clocks; chemical genetics; circadian rhythms; developmental genetics; flavoproteins; gene expression; gene mutation; genetic markers; genetic regulation; histones; nonvisual photoreceptor; phosphorylation; photobiology; plant genetics; plant growth /development; plant proteins; protein binding; protein protein interaction; protein structure function; receptor expression; solar radiation

DESCRIPTION (provided by applicant): Solar radiation is the ultimate energy source for all eukaryotes on earth, and it also provides information about the time, season, and surrounding environments. How organisms respond to light and how photosensory receptors mediate light responses represent some of the fundamental questions in biology. Cryptochrome is a blue/UV-A light receptor and the only photoreceptor known to function in both plants and animals. The function of cryptochromes is not limited to the control of plant photomorphogenetic development; their functions in the circadian clock and photoperiodism have also been found to affect complex human health problems such as cancer, sleep disorders, and other behavioral disorders. Investigation of how cryptochromes regulate plant development would increase our understanding of a fundamental aspect of life, and the knowledge resulting from such studies is also relevant to the improvement of human health. We have recently discovered that blue light-induced cryptochrome phosphorylation play key roles in the function and regulation of cryptochromes and identified approximate 100 primary target genes of CRY2 action. We have also developed the new genetics screens to identify mutations affecting cryptochrome functions, including sec (suppressors of cry1cry2), ecc (enhancers of cry1cry2), and coc (CRY2-GFP overexpression counteractor). We have cloned 9 SCC genes, and one ECC gene. The objectives of this proposal are (1) to investigate the biochemical mechanism of cryptochrome phosphorylation; (2) to study how CRY2 affects expressions of its putative primary target genes in response to blue light, and (3) to continue identify and clone the SCC, ECC, and COC genes, and to investigate how the SCC genes that we have identified, especially SCC4-D (a putative RING E3) and SCC5-D (a putative kinase), may mediate cryptochrome signal transduction in Arabidopsis.

描述（由申请人提供）：太阳辐射是地球上所有真核生物的最终能源，它还提供有关时间，季节和周围环境的信息。生物如何对光的反应以及光感受体如何介导光反应代表生物学中的一些基本问题。 加密色素是一种蓝色/UV-A光受体，也是唯一已知在植物和动物中起作用的光感受器。加密染色体的功能不仅限于控制植物的光生发育。还发现它们在昼夜节律时钟和光周期中的功能会影响复杂的人类健康问题，例如癌症，睡眠障碍和其他行为障碍。 对隐形植物如何调节植物发育的调查将增加我们对生活的基本方面的理解，而这些研究所产生的知识也与改善人类健康有关。 我们最近发现，蓝光诱导的加密磷酸化磷酸化在加密斑块的功能和调节中起关键作用，并确定了Cry2作用的大约100个主要靶基因。我们还开发了新的遗传筛选，以鉴定影响隐性功能的突变，包括SEC（CRY1CRY2的抑制剂），ECC（Cry1cry2的增强子）和COC（CRY2-GFP过表达反应器）。我们已经克隆了9个SCC基因和一个ECC基因。该提案的目标是（1）研究加密​​磷酸化的生化机制； （2）研究CRY2如何影响其假定的主要靶基因对蓝光的表达，（3）继续识别和克隆SCC，ECC和COC基因，并研究我们已经鉴定出的SCC4-D（尤其是scc4-d（尤其是推定的环E3）和SCC5-D-D-D-D-D-D-D（推定的Kinase）的SCC基因，可以使用AIDIADE CRYPROME CRYPORM CRYPORM CRYPROMS INCOCH INCOCH INCOCHICT INCOCHICT INCOCHICT INCOCHICT INCOCHICT INCOCHICT INCOCH INCOCH INCOCH INCOCH in in CRYPORM。