Plant Photosensory Receptor CRY2 Signaling Mechanism

植物光敏受体 CRY2 信号机制

基本信息

批准号：
7921233
负责人：
CHENTAO LIN
金额：
$ 17.25万
依托单位：
UNIVERSITY OF CALIFORNIA LOS ANGELES
依托单位国家：
美国
项目类别：
财政年份：
2009
资助国家：
美国
起止时间：
2009-09-30 至 2010-08-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/7921233
关键词：
Affect Animals Arabidopsis Bacteria Binding Biological Models Biology C-terminal Chemicals Collaborations Complex Crying Crystallization DNA Family member Flowers Fluorescence-Activated Cell Sorting Gene Expression Gene Expression Regulation Genes Genetic Genetic Screening Genetic Transcription Health Human In Vitro Individual Laboratories Light Luciferases Mediating Methods Modeling Molecular Mutation Organism Photoreceptors Plants Protein Binding Proteins RNA Cap-Binding Proteins RNA Splicing Recruitment Activity Regulation Signal Transduction Signaling Protein System Techniques Testing Time Transgenic Plants Translations Ubiquitination Work base cell type chemical genetics design experience genetic analysis in vivo mRNA Export mutant plant growth/development promoter protein complex protein degradation public health relevance receptor response tool yeast two hybrid system

Affect Animals Arabidopsis Bacteria Binding Biological Models Biology C-terminal Chemicals Collaborations Complex Crying Crystallization DNA Family member Flowers Fluorescence-Activated Cell Sorting Gene Expression Gene Expression Regulation Genes Genetic Genetic Screening Genetic Transcription Health Human In Vitro Individual Laboratories Light Luciferases Mediating Methods Modeling Molecular Mutation Organism Photoreceptors Plants Protein Binding Proteins RNA Cap-Binding Proteins RNA Splicing Recruitment Activity Regulation Signal Transduction Signaling Protein System Techniques Testing Time Transgenic Plants Translations Ubiquitination Work base cell type chemical genetics design experience genetic analysis in vivo mRNA Export mutant plant growth/development promoter protein complex protein degradation public health relevance receptor response tool yeast two hybrid system

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项目摘要

DESCRIPTION (provided by applicant): How organisms respond to light and how photosensory receptors mediate light responses are some of the most basic questions in biology that affect human health. Cryptochromes (CRY) are blue/UV-A light receptors found in bacteria, plants, and animals including human, but the molecular mechanisms of CRYs remain not well understood. My laboratory uses Arabidopsis as the model system to study signaling mechanisms underlying CRY1 and CRY2 regulation of plant development. We recently identified, for the first time, blue light-dependent CRY-interacting proteins in plants. We propose to continue study CRY1 and CRY2 signaling mechanisms with three specific aims. First, we propose to investigate how CRY-interacting proteins regulate transcription, mRNA export, and protein stability. Second, we plan to investigate CRY regulation of cell-type- specific gene expression changes. Third, we propose to identify and characterize genes affecting blue light-induced CRY2 degradation and blue light- suppressed CIB1 degradation. PUBLIC HEALTH RELEVANCE: Cryptochromes (CRY) are blue/UV-A light receptors regulating plant development and human health. We propose to study CRY-interacting proteins and how they mediate CRY regulation of gene expression in response to light.

描述（由申请人提供）：生物如何对光的反应以及光感受体如何介导光反应是影响人类健康的生物学中最基本的问题。加密染色体（Cry）是在包括人类在内的细菌，植物和动物中发现的蓝色/UV-A光受体，但裂解的分子机制仍未得到充分了解。我的实验室使用拟南芥作为模型系统来研究植物发育的CRY1和CRY2调节的信号传导机制。我们最近首次确定植物中的蓝色光依赖性哭泣蛋白。 We propose to continue study CRY1 and CRY2 signaling mechanisms with three specific aims.首先，我们建议研究如何调节转录，mRNA输出和蛋白质稳定性。其次，我们计划研究细胞型特异性基因表达变化的哭泣调节。第三，我们建议识别和表征影响蓝色光诱导的CRY2降解和蓝光抑制CIB1降解的基因。公共卫生相关性：隐形色素（CRY）是调节植物发育和人类健康的蓝色/UV-A光受体。我们建议研究哭泣的相互作用蛋白，以及它们如何介导基因表达的哭泣调节，以响应光。