Chiroptical Induced CPL-Based Tool as a Probe of Biological Substrates

基于手性光学诱导 CPL 的工具作为生物底物的探针

• 批准号: 9069485
• 负责人: Gilles Muller
• 金额: $ 10.76万
• 依托单位: SAN JOSE STATE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-01-01 至 2018-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/9069485
• 关键词: Acids; Affect; Amino Acids; Antibiotics; Binding; Biochemistry; Bioinorganic Chemistry; Biological; Biological Sciences; Biology; Bovine Serum Albumin; Characteristics; Chemicals; Complement; Complex; Development; Discrimination; Electron Transport Complex III; Environment; Europium; Exhibits; Fingerprint; Funding; Geometry; Grant; Health; Human; Image; Investigation; Ions; Knowledge; Laboratories; Lanthanoid Series Elements; Ligands; Light; Luminescent Measurements; Macromolecular Complexes; Malates; Metals; Methods; Modeling; Molecular; Molecular Conformation; Molecular Probes; Nature; Noise; Organic solvent product; Property; Proteins; Reporting; Research; Research Design; Research Project Grants; Sampling; Serum Albumin; Signal Transduction; Solvents; Source; Spectrum Analysis; Structure; Techniques; Technology; Tetracyclines; Therapeutic Uses; Tissues; United States National Institutes of Health; Variant; Work; aqueous; base; biological systems; chemical property; chromophore; design; interest; light scattering; luminescence; metal complex; molecular recognition; novel; sugar; tool

DESCRIPTION (provided by applicant): Circularly polarized luminescence (CPL)-active lanthanide(III) complexes present a new method for chiral distinction of biomolecules upon metal binding or structural arrangements. This study is envisaged to understand the significantly different photophysical and chiroptical properties of the europium(III)-tetracycline (Eu:TC) complex. It is worth noting that the TC class of antibiotics has achieved importance beyond their therapeutic use as broad-spectrum antibiotics because many metal complexes utilizing TC have been used as luminescent probes for the investigation of biomolecular interactions. The method involves understanding the (a) speciation of TC, (b) conformation and ternary complexes formed between the Eu:TC complexes in aqueous and/or organic solvents at varied pH values and (c) variation of this Eu:TC complex using various TC-like derivatives. By identifying the structural changes of the various Eu:TC complexes, this CPL-active luminescent molecular probe can be designed and studied in diverse solutions at targeted pH values to allow for the molecular and chiral recognition/sensing of biomolecules such as amino acids, sugar derivatives, proteins, etc. Using luminescence, 5D07F0 Eu(III) excitation, and CPL spectroscopy, will allow for the study of the Eu:TC complex to recognize and interact with different chiral compounds in solution. The results from this study will explicate information on the local microenvironment about the lanthanide(III) metal by understanding the interactions of added analytes, in reference to chiral biomolecules like L-malic acid, human serum albumin (HSA), and bovine serum albumin (BSA), on the Eu:TC probe. CPL spectroscopy is used as a "fingerprint" to indicate the structural changes within the Eu:TC complex as a result in a chiral signal correlated to the molecular and chiral sensing of an added biomolecule. The use of molecular probing using luminescent lanthanide(III) metals will aid in understanding the structural and functional aspects of each essential metal ion within many biological systems. In addition, the development of a straightforward and sensitive CPL-based technique for chiral elucidation has the potential to become a standard technique in biology and biochemistry laboratories, so the broader impacts are significant.

描述（由申请人提供）：循环极化发光（CPL）活性灯笼（III）配合物为金属结合或结构排列时生物分子的手性区别提供了一种新方法。该研究设想了解Europium（III） - 四环素（EU：TC）复合物的光学和手势性质的显着不同。值得注意的是，TC类抗生素的治疗用途超出其作为广谱抗生素的重要性，因为许多利用TC的金属配合物已被用作发光探针，以研究生物分子相互作用。 该方法涉及理解TC的（a）形成，（b）构象和在不同的pH值下于水和/或有机溶剂中形成的欧盟：TC复合物之间形成的三元络合物，以及（c）使用各种TC样衍生物的pH值以及（c）欧盟：TC复合物的变化。通过识别各种EU：TC复合物的结构变化，可以在有针对性的pH值的各种溶液中设计和研究这种CPL活性的发光分子探针，以允许对分子和手性识别/感应氨基酸，糖衍生物，蛋白质，蛋白质等，使用发光，5DD0 7F0 7F0 7F0 eu（II II eu（II II），为了研究欧盟：TC复合物，以识别溶液中不同手性化合物并相互作用。这项研究的结果将通过理解添加分析物的相互作用，即在EU：eu：tc Pibe上，通过了解添加分析物的相互作用（如L-麦酸酸，人血清白蛋白（HSA）和牛血清白蛋白（BSA）），在局部微环境上阐明有关兰坦德（III）金属的信息。 CPL光谱法被用作“指纹”，以指示欧盟：TC复合物内的结构变化，因为手性信号与添加的生物分子的分子和手性传感相关。使用发光灯笼（III）金属使用分子探测，将有助于理解许多生物系统中每个必需金属离子的结构和功能方面。此外，开发一种基于直接和敏感的基于CPL的手性阐明技术有可能成为生物学和生物化学实验室中的标准技术，因此更广泛的影响很大。