Using CPL for Enantioselective Recognition of Amino Acids

使用 CPL 进行氨基酸对映选择性识别

• 批准号: 7059555
• 负责人: Gilles Muller
• 金额: $ 12.66万
• 依托单位: SAN JOSE STATE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-01-01 至 2009-12-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7059555
• 关键词: aminoacid; chemical synthesis; ligands; luminescence; racemization; rare earth element; spectrometry; stereochemistry; stereoisomer; technology /technique development; aminoacid; chemical synthesis; ligands; luminescence; racemization; rare earth element; spectrometry; stereochemistry; stereoisomer; technology /technique development

The work described in this proposal is intended to serve as a starting point aimed at demonstrating that circularly polarized luminescence (CPL) spectroscopy would be an attractive complementary and/or alternative method to current techniques used as specific structural probes of chiral molecules. The initial efforts in this project will be to develop a technique to determine the absolute stereochemistry of simple chiral organic molecules such as amino acids. The emphasis here is the design of experiments aimed at understanding, improving, and maximizing the major non-covalent interactions governing the chiral discrimination between racemic lanthanide(lll) complexes and chiral organic molecules. Several aims will form the bulk of this proposal: (i) the ability to identify "ideal" racemic lanthanide complexes by designing and synthesizing appropriate ligands, (ii) the addition of chirality into our complex systems will improve the selectivity toward simple chiral molecules, (iii) a judicious change in the selected transition used for circular polarization excitation would overcome the limitation of the low intensity of the currently allowed-magnetic dipole chosen for CPL measurement, but will also considerably enhance the structural information gained from the Eu(lll) systems of interest, and (iv) the investigation of the importance of the various non-covalent chiral discriminatory interactions. The ultimate goal of this work is to be able to develop CPL spectroscopy as an important tool for biological applications.

本提案中描述的工作旨在作为起点，旨在证明 循环极化发光（CPL）光谱法将是一个有吸引力的互补和/或 用于当前技术的替代方法，用作手性分子的特定结构探针。最初 该项目的努力将是开发一种技术来确定简单的绝对立体化学 手性有机分子，例如氨基酸。这里的重点是针对实验的设计 理解，改善和最大化管理手性的主要非共价互动 歧视外表流灯笼（LLL）复合物和手性有机分子。有几个目标 构成该建议的大部分：（i）通过设计和 合成适当的配体，（ii）将手性添加到我们的复杂系统中将改善 对简单手性分子的选择性，（iii）用于圆形的选定过渡的明智变化 极化激发将克服当前允许磁性低强度的限制 选择用于CPL测量的偶极子，但也将大大增强获得的结构信息 来自欧盟（LLL）感兴趣的系统，（iv）调查各种非共价的重要性 手性歧视性相互作用。 这项工作的最终目标是能够开发CPL光谱作为生物学的重要工具 申请。