Development and Production of Standardized Reference Diets for Zebrafish Research

斑马鱼研究标准化参考饲料的开发和生产

• 批准号: 10823702
• 负责人: Donald Corace
• 金额: $ 129.2万
• 依托单位: MERIDIAN BIOTECH, LLC
• 依托单位国家: 美国
• 财政年份: 2023
• 资助国家: 美国
• 起止时间: 2023-09-15 至 2025-08-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10823702
• 关键词: Academia; Address; Adolescent; Adoption; Adult; Affect; Alabama; Amino Acids; Animal Model; Applied Research; Aquaculture; Biomass; Biomedical Research; Biotechnology; Businesses; Canada; Caseins; Collaborations; Coupled; Data; Development; Diet; Dietary Proteins; Disease; Dryness; Farm; Fatty Acids; Fatty acid glycerol esters; Fishes; Formulation; Gel; Genetic; Goals; Growth; Health; Human; Immunocompetence; Industry; Intake; Investments; Laboratories; Marketing; Meridians; Modeling; Modernization; Mus; Nutrient; Nutritional; Organism; Outcome; Penetration; Phase; Phenotype; Physiological Processes; Production; Progress Reports; Proteins; Reference Standards; Reproducibility; Research; Science; Shrimp; Small Business Innovation Research Grant; Source; Standardization; Technology; Testing; Toxicology; United States National Institutes of Health; Universities; Vertebrates; Work; Zebrafish; cohort; commercialization; feeding; fortification; improved; lipid metabolism; manufacturing capabilities; manufacturing scale-up; microbial; microbiome; offspring; pharmacologic; physical property; programs; reproductive; reproductive success; single cell proteins; transcriptome sequencing; trial design

Project Summary The over-arching goal of this Phase II SBIR proposal is to optimize and implement high quality standard reference diets for the Zebrafish (Danio rerio), an animal model of critical importance to the understanding of human health and development of vertebrate organisms. A key problem in the industry is that high quality standardized reference diets, with appropriate physical properties and adequate feed management strategies, have not been developed for D. rerio. Our solution for the industry, driven by a synergistic collaboration between Meridian Biotech and the laboratory of Dr. Stephen Watts at the University of Alabama at Birmingham, is to develop several open formulation diets for larval, juvenile and adult Zebrafish, which will achieve a desired level of control and whereby use of this modern and effective biomedical animal model is substantially improved and more standardized. This commercial-academic collaboration is a significant value add to this applied science program. Key ingredients in this formulated diet will include protein packages that contain defined and effective sources, including a microbial based Single Cell Protein (SCP), an enriched protein source obtained from bacterial biomass. Diets with these protein sources have been developed by Meridian Biotech, several of which have improved shrimp and fish farming feed technologies markedly; thus, we wish to apply this technology to D. rerio and, potentially, other relevant animal models. Our mutual Phase II Milestones and Specific Experimental Aims are many across both years of the program. Specific Aims for Milestone 1 (Year 1 work) include optimizing the protein content and amino acid profile of our most promising ingredient and testing it against the existing SCP-fortified diet used in pilot Phase I work, comparing the best diet to the competitor diets currently used in academic cores across the US biomedical research enterprise, and characterizing deeply at the phenotypic and genetic levels the benefits of our diet. Milestone 1 will seek to identify and progress the best diet and drive its market penetration, initially in academia. Specific Aims for Milestone 2 (Year 2 work) include further optimizing the amino acid, fatty acid, neutral fat, and energy of the SCP ingredient and scaling up manufacturing to meet demands at Meridian’s new US-based facilities, sharing the best diet formulation with academic Zebrafish cores to drive adoption of the diet, and centralizing the data gathering from the many academic cores for use by all and the NIH ORIP. Milestone 2 will seek to penetrate the academic Zebrafish husbandry marketplace by gaining Core adoption. In parallel with collaborative efforts within the academic marketplace, Meridian and commercial partners across both years will also pursue a Milestone 3 which seeks to expand the technologies and penetrate the industry fish aquaculture and farming marketplace, other production species, and potentially develop human applications.

项目摘要 这一II阶段SBIR提案的整个目标是优化和实施高质量标准 斑马鱼（Danio Rerio）的参考饮食，这是一种对理解的重要性的动物模型 人类健康和脊椎动物的发育。该行业的关键问题是高质量 具有适当的物理特性和足够的饲料管理策略的标准化参考饮食， 尚未为D. Rerio开发。我们的行业解决方案是在协同合作的推动下 在子午线生物技术和阿拉巴马大学斯蒂芬·瓦茨博士的实验室之间 伯明翰，将开发几种用于幼虫，少年和成人斑马鱼的开放式饮食，这将 达到所需的控制水平，并且使用这种现代有效的生物医学模型是 大大改善并更具标准化。这种商业学术的合作是一个重要的价值 加入该应用科学计划。这种配制饮食中的关键因素将包括蛋白质包装 包含定义有效的来源，包括基于微生物的单细胞蛋白（SCP），富集 从细菌生物量获得的蛋白质来源。这些蛋白质来源的饮食已经由 子午生物技术，其中一些已经改善了虾和养鱼饲料技术。因此， 我们希望将这项技术应用于D. Rerio以及其他相关动物模型。我们的共同阶段II 在该计划的两年中，里程碑和特定的实验目标都是许多。具体目标 里程碑1（1年工作）包括优化我们最有前途的蛋白质含量和氨基酸剖面 成分并针对试点I阶段工作中现有的SCP实体饮食进行测试，并比较最佳 饮食为目前在美国生物医学研究企业的学术核心中使用的竞争者饮食， 并深入表征表型和遗传水平我们饮食的好处。里程碑1将寻求 识别并进步最好的饮食，并推动其市场渗透，最初在学术界。具体目标 里程碑2（2年级工作）包括进一步优化氨基酸，脂肪酸，中性脂肪和能量 SCP成分并扩大制造业，以满足子午线新设施的需求，共享 具有学术斑马鱼核心的最佳饮食配方，可推动饮食的采用，并集中数据 从许多学术核心中聚集，以供所有人和NIH Orip使用。里程碑2将寻求穿透 通过获得核心采用，学术斑马鱼饲养市场。与协作努力并行 在学术市场中，这两年的子午线和商业伙伴也将追求 旨在扩展技术并渗透行业鱼类水产养殖和农业的里程碑3 市场，其他生产物种，并有可能发展人类的应用。