Scientific Core B

科学核心B

• 批准号: 8997973
• 负责人: Holden T. Maecker
• 金额: $ 36.58万
• 依托单位: STANFORD UNIVERSITY
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/8997973
• 关键词: Allergens; Allergy to peanuts; B-Lymphocytes; Basophils; Bioinformatics; Biological Assay; Biological Markers; Cytometry; Data; Data Analyses; Data Set; Databases; Development; Environment; Epitopes; Flow Cytometry; Human; Immune Tolerance; Immune system; Immunologic Monitoring; Immunophenotyping; Individual; Instruction; Leukocytes; Microfluidics; Mining; Patients; Peptide/MHC Complex; Peptides; Placebos; Population; Standardization; T-Lymphocyte; Technology; Testing; Whole Blood; Work; cohort; data integration; data management; data mining; functional genomics; innovation; novel; oral immunotherapy; technology development; tool

PROJECT SUMMARY (See instructions): By combining immune monitoring on multiple platforms in a single center, the Human Immune Monitoring Center (HIMC) has created an ideal environment for standardization, technology development, and data mining related to immune monitoring. Over the last few year's, we have built an array of high-throughput and high-content assays that are proving useful for a variety of immunological studies. The Scientific Core B will provide standardized immunological assays across studies within this proposed U19 project, focusing especially on the characterization of key populations of blood leukocytes, particularly B cells, T cells, and basophils, in patients with peanut allergy (PA) as they undergo treatment with oral immunotherapy (OIT) or placebo, as well as work with individual projects to develop additional novel immune monitoring assays. The specific aims are: 1. To provide standardized immune monitoring assays across projects. These will include detailed immunophenotyping of whole blood by mass cytometry (CyTOF), as well as phosphoepitope flow cytometry. By using the same standardized assays across all cohorts, we will greatly increase the power to test hypotheses related to any of the readouts of these assays. 2. To develop novel immune monitoring assays with individual projects. We will work with Project 3 on applications of MHC class ll-peptide multimer assays. These will be used in conjunction with microfluidic qPCR arrays, for comprehensive assessment of individual epitope-specific T cells. We will also develop phosphoepitope flow cytometry panels on the CyTOF mass cytometry platform. Once optimized, these technologies will be made available to other projects to create larger standardized data sets. 3. To facilitate mining of immune monitoring data. We will continue to expand our data integration and mining tool, Stanford Data Miner (SDM), for use with standard assays generated in this U19. We can also use SDM to compare U19 data with data generated with the same standardized assays in unrelated projects. We will work with the Data Management and Analysis Core A to mine those data using bioinformatics tools.

项目摘要（请参阅说明）： 通过在单个中心的多个平台上结合免疫监测，人类免疫监测中心（HIMC）为与免疫监测有关的标准化，技术开发和数据挖掘创造了理想的环境。在过去的几年中，我们建立了一系列高通量和高素质测定法，这些测定法对各种免疫学研究有用。科学核心B将在该提出的U19项目中的研究中提供标准化的免疫学测定，特别是在患有花生过敏（PA）患者的血液白细胞，尤其是B细胞，T细胞和嗜碱力的关键种群的表征上，因为它们可以接受口服免疫疗法（OIT）或其他项目的疾病，以进行其他项目，以进行其他项目的疾病。具体目的是： 1。在项目中提供标准化的免疫监测测定法。这些将包括通过质量细胞术（Cytof）以及磷尖质流式细胞术对全血的详细免疫表型。通过在所有队列中使用相同的标准化测定法，我们将大大提高测试与这些测定的任何读数相关的假设的能力。 2。通过单个项目开发新颖的免疫监测测定法。我们将使用项目3的MHC类LL肽多聚机测定的应用。这些将与微流体QPCR阵列一起使用，以全面评估单个表位特异性T细胞。我们还将在细胞质量细胞仪平台上开发磷酸化流式细胞术面板。一旦优化，这些技术将被提供给其他项目以创建更大的标准化数据集。 3。促进采矿免疫监测数据。我们将继续扩展我们的数据集成和采矿工具Stanford Data Miner（SDM），以与本U19中生成的标准测定法一起使用。我们还可以使用SDM将U19数据与无关项目中相同标准化测定的数据进行比较。我们将使用数据管理和分析核心A使用生物信息学工具来挖掘这些数据。