Multi-User Time-Resolved Fluorescence Spectrometer

多用户时间分辨荧光光谱仪

• 批准号: 8825781
• 负责人: Joseph R. LAKOWICZ
• 金额: $ 24.34万
• 依托单位: UNIVERSITY OF MARYLAND BALTIMORE
• 依托单位国家: 美国
• 财政年份: 2015
• 资助国家: 美国
• 起止时间: 2015-03-01 至 2016-02-29
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8825781
• 关键词: Anisotropy; Baltimore; Biochemistry; Biological; Collection; Computer software; Computers; Contract Services; Event; Fees; Fluorescence; Fluorescence Polarization; Fluorescence Resonance Energy Transfer; Fluorescence Spectroscopy; Frequencies; Funding; Grant; Maryland; Measurement; Methods; Minor; Molecular Biology; Optics; Peer Review; Principal Investigator; Publications; Research; Research Personnel; Scientist; Time; United States National Institutes of Health; Universities; base; design; instrument; instrumentation; medical schools; optical spectra; tau Proteins; Anisotropy; Baltimore; Biochemistry; Biological; Collection; Computer software; Computers; Contract Services; Event; Fees; Fluorescence; Fluorescence Polarization; Fluorescence Resonance Energy Transfer; Fluorescence Spectroscopy; Frequencies; Funding; Grant; Maryland; Measurement; Methods; Minor; Molecular Biology; Optics; Peer Review; Principal Investigator; Publications; Research; Research Personnel; Scientist; Time; United States National Institutes of Health; Universities; base; design; instrument; instrumentation; medical schools; optical spectra; tau Proteins

DESCRIPTION (provided by applicant): The Center for Fluorescence Spectroscopy (CFS) has provided time-resolved measurements to scientists on this campus since 1988, resulting in over 200 peer-reviewed publications. This instrumentation is now obsolete or nonfunctional. NIH-funded principal investigators (PIs) at the University of Maryland, Baltimore (UMB) have a scientific need for a state-of-the-art fluorescence lifetime spectrofluorometer to support and enhance their NIH funded research. The requested instrument is a modern recently developed computer controlled spectrofluorometer which performs automated time-resolved measurements. The FluoTime 300 "Easy Tau" is a new instrument from PicoQuant designed to make time-resolved measurements easily available to biological researchers. This instrument will be located in and managed by the Center for Fluorescence Spectroscopy (CFS) at UMB and will be available for PIs throughout UMB. This instrument will provide automated collection of fluorescence intensity, fluorescence polarization (FP), anisotropy decays, time- resolved emission spectra, decay-associated spectra, and fluorescence resonance energy transfer (FRET). At present we have limited capabilities for time-resolved measurements. We have one LED-based frequency- domain (FD) instrument with very low sensitivity and one time-domain instrument which uses only optical filters for wavelength selection. These instruments can only be operated by spectroscopists and cannot support the demand for time-resolved measurements of UMB. The CFS has extensive software for time-resolved fluorescence using the frequency-domain method. With support from the Department of Biochemistry and Molecular Biology this valuable software will be modified for TCSPC measurements. The School of Medicine guarantees support for the service contract for five years in the event of a shortage from user fees. The PicoQuant FT300 will be used 100% of the time by 10 major users supported by 14 NIH grants and by 8 minor users supported by 3 NIH grants.

描述（由申请人提供）：自1988年以来，荧光光谱中心（CFS）为该校园的科学家提供了时间分辨的测量，从而导致了200多个经过同行评审的出版物。现在，该仪器已过时或无功能。巴尔的摩大学（UMB）的NIH资助的首席研究人员（PIS）对先进的荧光寿命光谱仪的科学需求，以支持和增强其NIH资助的研究。请求的仪器是一种现代开发的计算机控制的光谱仪，可执行自动分辨时间分辨的测量。 Fluotime 300“ Easy Tau”是一种来自Picoquant的新仪器，旨在使生物学研究人员易于获得时间分辨的测量。该仪器将由UMB的荧光光谱中心（CFS）放置并管理，并将用于整个UMB的PI。该仪器将提供荧光强度，荧光极化（FP），各向异性衰减，时间分辨的发射光谱，与衰减相关的光谱和荧光共振能传递（FRET）的自动收集。目前，我们对时间分辨测量的功能有限。我们有一种具有非常低灵敏度的基于LED的频域（FD）仪器，并且仅使用光学过滤器进行波长选择。这些仪器只能由光谱学家操作，不能支持对UMB的时间分辨测量的需求。 CFS使用频域方法具有广泛的用于时间分辨荧光的软件。在生物化学和分子生物学系的支持下，该有价值的软件将用于TCSPC测量。医学学院在使用用户费用短缺的情况下保证对服务合同的支持已有五年。 Picoquant FT300将在100％的时间内使用10个主要用户，并由14个NIH赠款支持，并由3个NIH赠款支持的8个未成年人使用。