Photonics-based Fluorescence Imaging for Research, Diagnostics, and Pathology

用于研究、诊断和病理学的基于光子学的荧光成像

基本信息

批准号：
10329143
负责人：
Joseph R. LAKOWICZ
金额：
$ 38.63万
依托单位：
UNIVERSITY OF MARYLAND BALTIMORE
依托单位国家：
美国
项目类别：
财政年份：
2022
资助国家：
美国
起止时间：
2022-02-01 至 2027-01-31
项目状态：
未结题

来源：
https://reporter.nih.gov/project-details/10329143
关键词：
2019-nCoV Area Binding Biological Assay Biological Sciences Cell membrane Cells Cellular Phone Centenarian Collaborations Complex Coupled Detection Devices Diagnostic Dimensions Electronics Fiber Optics Flow Cytometry Fluorescence Fluorescence Microscopy Generations Goals HIV Image Imaging Device Immunoassay Light Measurement Medicine Microscope Optics Pathology Pharmaceutical Preparations Photons Positioning Attribute Property Research Slide Specimen Structure Technology Transistors Virion Virus base brain tissue cellular imaging clinical application consumer product detector drug discovery fluorescence imaging fluorescence microscope fluorophore genetic testing high throughput screening imaging detector in vivo instrument lens medical schools multi-photon pathology imaging photonics plasmonics point-of-care diagnostics senior faculty whole slide imaging

项目摘要

Abstract - Photonics-based Fluorescence Imaging for Research, Diagnostics and Pathology During the past several decades fluorescence detection has become a central technology throughout the biosciences. The basic applications include studies of biomolecule function, properties of cell membranes and localization of target molecules in cells. The more clinical applications include immunoassays, flow cytometry, point-of-care diagnostics, genetic testing, and cell imaging by fluorescence microscopy. Fluorescence is expanding to include in-vivo measurements on brain tissues using multi-photon excitation. While fluorescence technology has advanced, it has not kept pace with the advances in electronics and array detectors (cameras). The sizes of optical components such as lenses and filters are much larger than electronic components as can be seen from a cell phone with millions of transistors, but only one or two lenses in a cell phone. This mismatch in size cannot be circumvented by making smaller lenses, filters or fiber optics. These optical components require dimensions of many wavelengths to manipulate freely propagating light. We propose to overcome this limitation by using fluorophores positioned within sub-wavelength near-field distances from the plasmonic, photonic or plasmonic multi-layer structures (MLS). We are NOT proposing to use the fluorophores as electronic components, but rather to directly couple their emission into CMOS imaging detectors with MLSs without free-space propagation of light. The MLS controls the propagation of optical energy, can separate wavelengths and can direct the energy (coupled photons) towards nearby detectors. This concept will provide the basis for new devices for research and medicine. To demonstrate the usefulness of these devices we have established collaborations with senior faculty in the School of Medicine. These collaborations include detection of weak binding in drug discovery or high throughput screening (HTS) because much of the HTS is used with drug fragments which bind weakly to target molicules. Most of the MLS retain spatial information in the x-y plane which allows either ensemble or virus particle counting assays for HIV and the Covid-19 virus SARS-CoV-2. The wide field of view will allow whole slide imaging of pathology specimens. Our goal is to develop this new area of near-field effects in fluorescence, with easy to fabricate strruvtures, to enable a new generation of instruments and devices for fluorescence detection in research, sensing and imaging.

摘要 - 基于光子学的荧光成像，用于研究，诊断和病理在过去的几十年中生物科学。基本应用包括生物分子功能的研究，细胞膜的特性和细胞中靶分子的定位。临床应用越多，包括免疫测定，流式细胞仪，通过荧光显微镜检查的诊断点诊断，基因检测和细胞成像。荧光是扩展到使用多光子激发对脑组织的体内测量。尽管荧光技术已经进步，但它尚未跟上电子设备的进步和阵列检测器（相机）。光学组件（例如镜头和过滤器）的尺寸大于从带有数百万晶体管的手机可以看到电子组件，但只有一个或两个镜头在手机中。通过制作较小的镜头，过滤器或光纤元件，不能规避这种不匹配的大小。这些光学成分需要许多波长的尺寸来操纵自由传播光。我们建议通过使用位于近场近波内的荧光团克服这一限制距离等离子，光子或等离子多层结构（MLS）的距离。我们不建议使用荧光团作为电子成分，而是将其发射直接置于CMOS成像中带有MLSS的检测器，没有光的空间传播。 MLS控制光能的传播，可以将波长分开，并可以将能量（耦合光子）引导到附近的探测器。这个概念将为研究和医学的新设备提供基础。为了证明这些设备的有用性，我们已经与高级教师建立了合作医学院。这些合作包括检测药物发现或高的结合。吞吐量筛选（HTS），因为许多HTS都与药物片段一起使用，这些药物片段与靶标有弱结合莫利奇。大多数MLS将空间信息保留在X-Y平面上，该信息允许合奏或病毒粒子对HIV和COVID-19病毒SARS-COV-2进行计数测定。广泛的视野将允许整个幻灯片成像病理标本。我们的目标是在荧光中开发这个新的近场效应区域，并易于制造，易于制造。为了在研究，传感和成像。