Needle biopsy preservation and preparation for rapid 3D pathology of pancreas

胰腺快速 3D 病理学针吸活检保存和准备

• 批准号: 8912438
• 负责人: Eric J Seibel
• 金额: $ 20.16万
• 依托单位: UNIVERSITY OF WASHINGTON
• 依托单位国家: 美国
• 财政年份: 2014
• 资助国家: 美国
• 起止时间: 2014-08-14 至 2017-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8912438
• 关键词: Affect; Architecture; Area; Automation; Biochemical Genetics; Biological Preservation; Biomedical Engineering; Biopsy; Biopsy Specimen; Breast; Cancerous; Cells; Chemicals; Clinical; Computer Assisted; Confocal Microscopy; Core Biopsy; Custom; Diagnosis; Diagnostic; Diffusion; Dimensions; Disease; Dyes; Evaluation; Fixatives; Fluorescence; Future; Goals; Gold; Hand; Health; Hematoxylin and Eosin Staining Method; Image; Imagery; Imaging Techniques; Lead; Lesion; Lung; Malignant Neoplasms; Malignant neoplasm of liver; Malignant neoplasm of lung; Malignant neoplasm of pancreas; Malignant neoplasm of thyroid; Measurement; Measures; Mechanics; Methods; Microfluidic Microchips; Microfluidics; Microscopy; Modality; Morphology; Needle biopsy procedure; Needles; Nuclear; Operative Surgical Procedures; Optical Coherence Tomography; Optics; Outcome; Pancreas; Pathologic; Pathologist; Pathology; Patients; Physicians; Preparation; Procedures; Process; Prostate; Protocols documentation; Radiology Specialty; Recurrence; Resolution; Sampling; Sensitivity and Specificity; Site; Slice; Slide; Solid Neoplasm; Solutions; Specimen; Staging; Staining method; Stains; Structure; Surface; Technology; Testing; Thick; Three-Dimensional Imaging; Thyroid Gland; Time; Tissues; Translating; Translational Research; Ultrasonography; acoustic imaging; brain surgery; cancer diagnosis; cancer invasiveness; clinical practice; cost; cost effective; experience; improved; in vivo; light scattering; meetings; novel; optical imaging; outcome forecast; personalized medicine; quantum; sample fixation; three dimensional structure; tissue preparation; two-dimensional; two-photon

DESCRIPTION (provided by applicant): There are over three million needle biopsies performed every year in the USA, typically for the diagnosis of breast, prostate, thyroid, lung, liver, and pancreatic cancers. Because needle biopsies are less invasive than surgical biopsies, they are much more cost-effective. However, a major limitation for all needle biopsies are that the pathologist or cytologist views the tissue or cells in only two dimensions, while the important structures for determining cancer and invasiveness of the disease is more clearly seen in threes dimensions (3D) versus two dimensions. What is true in radiology is expected in pathology, 3D imaging will give a quantum increase in the information content, expected to increase sensitivity and specificity of early cancer diagnosis. For many cases such as suspected pancreatic cancer, the small needle biopsy is all the tissue that the clinical team has to test before making a lifesaving decision. In this proposal, the preferred specimen for pancreatic cancer diagnosis is a thin needle core biopsy, which the entire 1-2cm long specimen is visualized in 3D using advanced optical imaging. After needle procurement from ex vivo pancreas, all handling and preparation for highest quality 3D imaging will be prototyped and validated within a novel microfluidics device. Every tissue preparatory step of fixing, staining, clearing, and transporting to 3D imaging is conducted within the microfluidics channel, which can be automated. The goal of this project is to reduce the time for biopsy tissue preparation to less than half the standard time in a pathology lab. Furthermore, the 3D architecture of the tissue will be preserved for the first opto-mechanical measures of biopsy intactness. The diagnostic value of the needle biopsy specimens after automated preparation will be determined by experienced pathologists. The ability to visualize 3D morphology within tissue biopsies will also allow the pathologists to bette compare ex vivo diagnosis with new in vivo 3D imaging. These ex vivo technologies that rely on optically cleared tissue and traditional bright-field imaging (Z-stack imaging and optical projection tomographic microscopy) can become the pathologists' bridge for better understanding in vivo 3D imaging technologies, such as confocal, optical coherence tomography, and photo-acoustic imaging. This project is expected to lead to a transformation in pathology from 2D to 3D, and in the ability to provide less invasive, low-cost and rapid cancer diagnosis, directly affecting several millions of US citizens per year.

描述（由申请人提供）：美国每年进行超过三百万次穿刺活检，通常用于诊断乳腺癌、前列腺癌、甲状腺癌、肺癌、肝癌和胰腺癌。由于针吸活检比手术活检侵入性更小，因此更具成本效益。然而，所有针活检的一个主要限制是病理学家或细胞学家仅以二维方式观察组织或细胞，而重要的 与二维相比，在三维 (3D) 中可以更清楚地看到用于确定癌症和疾病侵袭性的结构。放射学中的真实情况在病理学中也是如此，3D 成像将大幅增加信息内容，有望提高早期癌症诊断的灵敏度和特异性。对于许多病例，例如疑似胰腺癌，小针活检是临床团队在做出救生决定之前必须测试的所有组织。在该提案中，诊断胰腺癌的首选标本是细针活检，使用先进的光学成像技术对整个 1-2 厘米长的标本进行 3D 可视化。从离体胰腺采购针头后，最高质量 3D 成像的所有处理和准备工作都将在新型微流体设备中进行原型设计和验证。固定、染色、透明和运输的每个组织准备步骤 3D 成像是在微流体通道内进行的，可以实现自动化。该项目的目标是将活检组织准备时间减少到病理实验室标准时间的一半以下。此外，组织的 3D 结构将被保留，用于活检完整性的首次光学机械测量。自动制备后的针吸活检标本的诊断价值将由经验丰富的病理学家确定。在组织活检中可视化 3D 形态的能力也将使病理学家能够更好地将离体诊断与新的体内 3D 成像进行比较。这些依赖光学透明组织和传统明场成像（Z 堆栈成像和光学投影断层扫描显微镜）的离体技术可以成为病理学家更好地理解体内 3D 成像技术的桥梁，例如共焦、光学相干断层扫描、和光声成像。该项目预计将导致病理学从 2D 到 3D 的转变，并能够提供侵入性较小、低成本和快速的癌症诊断，每年直接影响数百万美国公民。