Needle biopsy preservation and preparation for rapid 3D pathology of pancreas

胰腺快速 3D 病理学针吸活检保存和准备

• 批准号: 8737000
• 负责人: Eric J Seibel
• 金额: $ 23.26万
• 依托单位: UNIVERSITY OF WASHINGTON
• 依托单位国家: 美国
• 财政年份: 2014
• 资助国家: 美国
• 起止时间: 2014-08-14 至 2017-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8737000
• 关键词: Affect; Architecture; Area; Automation; Biochemical Genetics; Biological Preservation; Biomedical Engineering; Biopsy; Biopsy Specimen; Breast; Cancerous; Cells; Chemicals; Clinical; Computer Assisted; Confocal Microscopy; Core Biopsy; Custom; Diagnosis; Diagnostic; Diffusion; Dimensions; Disease; Dyes; Evaluation; Fixatives; Fluorescence; Future; Goals; Gold; Hand; Hematoxylin and Eosin Staining Method; Image; Imagery; Imaging Techniques; Lead; Lesion; Lung; Malignant Neoplasms; Malignant neoplasm of liver; Malignant neoplasm of lung; Malignant neoplasm of pancreas; Malignant neoplasm of thyroid; Measurement; Measures; Mechanics; Methods; Microfluidic Microchips; Microfluidics; Microscopy; Modality; Morphology; Needle biopsy procedure; Needles; Nuclear; Operative Surgical Procedures; Optical Coherence Tomography; Optics; Outcome; Pancreas; Pathologic; Pathologist; Pathology; Patients; Physicians; Preparation; Procedures; Process; Prostate; Protocols documentation; Radiology Specialty; Recurrence; Resolution; Sampling; Sensitivity and Specificity; Site; Slice; Slide; Solid Neoplasm; Solutions; Specimen; Staging; Staining method; Stains; Structure; Surface; Technology; Testing; Thick; Three-Dimensional Imaging; Thyroid Gland; Time; Tissues; Translating; Translational Research; Ultrasonography; acoustic imaging; brain surgery; cancer diagnosis; cancer invasiveness; clinical practice; cost; cost effective; experience; improved; in vivo; light scattering; meetings; novel; optical imaging; outcome forecast; public health relevance; quantum; sample fixation; three dimensional structure; tissue preparation; two-dimensional; two-photon

DESCRIPTION (provided by applicant): There are over three million needle biopsies performed every year in the USA, typically for the diagnosis of breast, prostate, thyroid, lung, liver, and pancreatic cancers. Because needle biopsies are less invasive than surgical biopsies, they are much more cost-effective. However, a major limitation for all needle biopsies are that the pathologist or cytologist views the tissue or cells in only two dimensions, while the important structures for determining cancer and invasiveness of the disease is more clearly seen in threes dimensions (3D) versus two dimensions. What is true in radiology is expected in pathology, 3D imaging will give a quantum increase in the information content, expected to increase sensitivity and specificity of early cancer diagnosis. For many cases such as suspected pancreatic cancer, the small needle biopsy is all the tissue that the clinical team has to test before making a lifesaving decision. In this proposal, the preferred specimen for pancreatic cancer diagnosis is a thin needle core biopsy, which the entire 1-2cm long specimen is visualized in 3D using advanced optical imaging. After needle procurement from ex vivo pancreas, all handling and preparation for highest quality 3D imaging will be prototyped and validated within a novel microfluidics device. Every tissue preparatory step of fixing, staining, clearing, and transporting to 3D imaging is conducted within the microfluidics channel, which can be automated. The goal of this project is to reduce the time for biopsy tissue preparation to less than half the standard time in a pathology lab. Furthermore, the 3D architecture of the tissue will be preserved for the first opto-mechanical measures of biopsy intactness. The diagnostic value of the needle biopsy specimens after automated preparation will be determined by experienced pathologists. The ability to visualize 3D morphology within tissue biopsies will also allow the pathologists to bette compare ex vivo diagnosis with new in vivo 3D imaging. These ex vivo technologies that rely on optically cleared tissue and traditional bright-field imaging (Z-stack imaging and optical projection tomographic microscopy) can become the pathologists' bridge for better understanding in vivo 3D imaging technologies, such as confocal, optical coherence tomography, and photo-acoustic imaging. This project is expected to lead to a transformation in pathology from 2D to 3D, and in the ability to provide less invasive, low-cost and rapid cancer diagnosis, directly affecting several millions of US citizens per year.

描述（由申请人提供）：美国每年进行了超过三百万个针头活检，通常用于诊断乳房，前列腺，甲状腺，肺，肝脏和胰腺癌。由于针头活检不如手术活检侵入性，因此它们的成本效益要高得多。但是，所有针头活检的主要局限性是病理学家或细胞学家仅在两个维度上观察组织或细胞，而重要的是重要的 在三个维度（3D）与二维相对于二维中，确定疾病的癌症和侵入性的结构更清楚。预期在病理学中，放射学中的真实情况是，3D成像将使信息含量的量子增加，预计会增加早期癌症诊断的敏感性和特异性。在许多情况下，例如怀疑的胰腺癌，小针头活检是临床团队在做出救生决定之前必须测试的所有组织。在此提案中，胰腺癌诊断的首选标本是薄的针核活检，使用先进的光学成像在3D中可视化整个1-2cm长标本。在从体内胰腺采购的针头采购后，将在新型的微流体设备中对所有最高质量3D成像进行所有处理和制备。固定，染色，清除和运输的每个组织预备步骤 至3D成像是在微流体通道中进行的，可以自动化。该项目的目的是将活检组织制备的时间减少到病理实验室中标准时间的一半。此外，将保留组织的3D体系结构，以进行活检完整的第一光学机械测量。自动化制剂后针头活检标本的诊断值将由经验丰富的病理学家确定。在组织活检中可视化3D形态的能力还将使病理学家将离体诊断与新体内3D成像进行比较。这些依赖于光学清除组织和传统的明亮场成像（Z-stack Imaging和光影投影显微镜）的离体技术可以成为病理学家的桥梁，以在Vivo 3D成像技术中更好地理解，例如共焦，光学相干性层理，，光学相干性整洁，，和照片声学成像。预计该项目将导致病理学从2D到3D的转变，并能够提供侵入性，低成本和快速癌症诊断的能力，直接影响每年数百万美国公民。