Animal care: supporting research on pathogenesis and treatment of autoimmunity

动物护理：支持自身免疫发病机制和治疗的研究

• 批准号: 8158460
• 负责人: Massimo Gadina
• 金额: $ 111.5万
• 依托单位: NATIONAL INSTITUTE OF ARTHRITIS AND MUSCULOSKELETAL AND SKIN DISEASES
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/8158460
• 关键词: Autoimmunity; Pathogenesis; Research Support; animal care

The LACU assists the NIAMS IRP investigators with their research involving animals. Such animals are housed in shared animal facilities on the NIH campus including the NIAMS LACU directly overseen facility located in Building 10 in 9/C127 in the ACRF Buildings 6B, 10A, 50 and the Building 14 complex. NIAMS animal users work primarily with mice and rats. The NIAMS IRP Animal Program has oversight for 20 Animal Study Proposals (ASPs) using mouse and rat models. The LACU assists researchers prepare ASPs and has 3 voting members and 1 alternate on the NIAMS Animal Care and Use Committee (ACUC) and maintains the foster colony and the sentinel program for the IRP. Projects include arthritis, allergic rhinitis and other transmit receptor-induced cellular responses, recombination/chromatin remodeling using AID transgenic mice, familial Mediterranean fever (FMF), and TNF receptor associated periodic syndrome. Research involves muscle and related diseases (experimentation with: N-RAP protein, glycogen storage disease Type II, SIRT1 gene, myofibril assembly, and myositis) and sub-cellular organelles and cytoskeleton in skeletal muscle as well as in vitro experiments examining retroviral transduction, bone marrow transplantation, and epidermal differentiation using fetal and adult skin tissues. Many of the institutes studies involve apoptosis and autoimmunity, as well as cartilage studies focused on tissue-engineered cartilage, chondrogenesis, GDF-F, COMP and osteoarthritis. The NIAMS IRP LACU facilitated the accreditation of NIH with the Association for the Assessment and Accreditation of Laboratory Animal Care International (AALACi) during the program review and inspection in 2007, the next inspection will be in early summer of 2011. A proposal to renovate the 10/9C127 Animal Facility has been accepted and the physical renovation will start soon. This will add 720 additional mouse cages to the existing facility.

LACU 协助 NIAMS IRP 调查人员进行涉及动物的研究。这些动物被安置在 NIH 园区的共享动物设施中，包括位于 ACRF 6B、10A、50 号楼 9/C127 10 号楼的 NIAMS LACU 直接监管设施以及 14 号楼综合体。 NIAMS 动物用户主要与小鼠和大鼠一起工作。 NIAMS IRP 动物计划监督 20 项使用小鼠和大鼠模型的动物研究提案 (ASP)。 LACU 协助研究人员准备 ASP，并在 NIAMS 动物护理和使用委员会 (ACUC) 中拥有 3 名投票成员和 1 名候补成员，并维护 IRP 的寄养群体和哨兵计划。 项目包括关节炎、过敏性鼻炎和其他传输受体诱导的细胞反应、使用 AID 转基因小鼠进行重组/染色质重塑、家族性地中海热 (FMF) 和 TNF 受体相关周期性综合症。研究涉及肌肉和相关疾病（实验：N-RAP 蛋白、糖原贮积病 II 型、SIRT1 基因、肌原纤维组装和肌炎）和骨骼肌中的亚细胞细胞器和细胞骨架，以及检查逆转录病毒转导的体外实验，骨髓移植以及使用胎儿和成人皮肤组织的表皮分化。该研究所的许多研究涉及细胞凋亡和自身免疫，以及侧重于组织工程软骨、软骨形成、GDF-F、COMP 和骨关节炎的软骨研究。 NIAMS IRP LACU 在 2007 年的项目审查和检查期间促进了 NIH 获得国际实验动物护理评估和认证协会 (AALACi) 的认证，下一次检查将于 2011 年初夏进行。 10/9C127 动物设施已被验收，实体改造即将开始。 这将为现有设施增加 720 个额外的鼠笼。