Animal care: supporting research on autoimmune, inflammatory and muscle diseases

动物护理：支持自身免疫、炎症和肌肉疾病的研究

基本信息

批准号：
8940198
负责人：
Massimo Gadina
金额：
$ 170.81万
依托单位：
NATIONAL INSTITUTE OF ARTHRITIS AND MUSCULOSKELETAL AND SKIN DISEASES
依托单位国家：
美国
项目类别：
财政年份：
资助国家：
美国
起止时间：
至
项目状态：
未结题

来源：
https://reporter.nih.gov/project-details/8940198
关键词：
Accreditation Adult Affect Allergic rhinitis Ambulatory Care Animal Care and Use Committees Animal Diseases Animal Housing Animals Apoptosis Area Arthritis Autoimmune Process Autoimmunity Bone Development Bone Marrow Transplantation Bone Regeneration Cartilage Chondrogenesis Cytoskeleton Degenerative polyarthritis Disease Ensure Equipment and supply inventories Exclusion Familial Mediterranean Fever Fostering Genes Genetic Recombination Glycogen storage disease type II Guidelines Hypersensitivity In Vitro Inflammatory Institutes International Laboratory Animals Modeling Mus Muscle Muscle Development Myofibrils Myositis National Institute of Arthritis and Musculoskeletal and Skin Diseases Organelles Process Proteins Rattus Regulation Research Research Personnel Research Support Sentinel Site Visit Skeletal Muscle Skin Skin Tissue Syndrome Tissue Engineering Transgenic Mice Tumor Necrosis Factor Receptor United States National Institutes of Health Voting Work animal care animal facility chromatin remodeling expectation fetal meetings member programs receptor research facility research study response retroviral transduction

项目摘要

The LACU assists the NIAMS IRP investigators with their research involving animals. NIAMS animals housed in animal facilities located on the NIH campus, including the NIAMS LACU facility located in Building 10, room 9C127 (the Ambulatory Care Research Facility (ACRF)), as well as in Buildings 6B, 10A, 50 and Building 14. NIAMS animal users work primarily with mice and rats. The NIAMS IRP Animal Program has oversight for 20 Animal Study Proposals (ASPs) using mouse and rat models. The LACU assists researchers with preparing ASPs and has 3 members (1 alternate and 2 voting) on the NIAMS Animal Care and Use Committee (ACUC). The LACU maintains current detailed animal inventory, the foster colony and the sentinel program for the IRP animals. IRP project areas include arthritis, allergic rhinitis and other transmit receptor-induced cellular responses, recombination/chromatin remodeling using AID transgenic mice, familial Mediterranean fever (FMF), and TNF receptor associated periodic syndrome. Research involves muscle and related diseases (experimentation with: N-RAP protein, glycogen storage disease Type II, SIRT1 gene, myofibril assembly, and myositis) and sub-cellular organelles and cytoskeleton in skeletal muscle, as well as in vitro experiments examining retroviral transduction, bone marrow transplantation, and epidermal differentiation using fetal and adult skin tissues. Many of the institutes studies involve apoptosis and autoimmunity, as well as cartilage studies focused on tissue-engineered cartilage, chondrogenesis, GDF-F, COMP and osteoarthritis. The 10/9C127 Animal Facility prepared for and participated in the successful NIH wide 2014 AAALAC site visit.

LACU 协助 NIAMS IRP 调查人员进行涉及动物的研究。 NIAMS 动物饲养在 NIH 园区的动物设施中，包括位于 10 号楼 9C127 室（流动护理研究设施 (ACRF)）的 NIAMS LACU 设施，以及 6B、10A、50 号楼和 14 号楼。动物使用者主要与小鼠和大鼠一起工作。 NIAMS IRP 动物计划监督 20 项使用小鼠和大鼠模型的动物研究提案 (ASP)。 LACU 协助研究人员准备 ASP，并在 NIAMS 动物护理和使用委员会 (ACUC) 中拥有 3 名成员（1 名候补成员和 2 名投票成员）。 LACU 维护着当前详细的动物库存、IRP 动物的寄养群和哨兵计划。 IRP 项目领域包括关节炎、过敏性鼻炎和其他传输受体诱导的细胞反应、使用 AID 转基因小鼠进行重组/染色质重塑、家族性地中海热 (FMF) 和 TNF 受体相关周期性综合症。研究涉及肌肉和相关疾病（实验：N-RAP 蛋白、糖原累积病 II 型、SIRT1 基因、肌原纤维组装和肌炎）和骨骼肌中的亚细胞细胞器和细胞骨架，以及检查逆转录病毒转导的体外实验、骨髓移植以及使用胎儿和成人皮肤组织的表皮分化。该研究所的许多研究涉及细胞凋亡和自身免疫，以及侧重于组织工程软骨、软骨形成、GDF-F、COMP 和骨关节炎的软骨研究。 10/9C127 动物设施准备并参与了 NIH 范围内的 2014 年 AAALAC 现场访问的成功。