Shape change and Nitric Oxide (NO) Modulation of Core Pluripotent TF Expression

核心多能 TF 表达的形状变化和一氧化氮 (NO) 调节

• 批准号: 8157692
• 负责人: John Jessup
• 金额: $ 5.51万
• 依托单位: DIVISION OF BASIC SCIENCES - NCI
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/8157692
• 关键词: Nitric Oxide; Shapes

Our initial postulate may still be correct but our focus shifted during the year to a more unbiased transcriptomic approach. We have performed whole transcriptomic analysis of the genes expressed in 7 day monolayer and spheroid cultures of Clone A and CX-1. The data are still under evaluation but suggest that there are 462 genes upregulated in both Clone A and CX-1 spheroids with 249 genes up in Clone A but down in CX-1 with 1,195 genes up in CX-1 down in Clone A and 1,861 genes down in CX-1 and Clone A. These changes are out of a total of more than 29,000 genes in the reference sequence list and the addition of several important isoforms of genes that are not present in last refSeq build. We are now in the process of determining what pathways are most affected. Our focus is on identification of pathways such as cell proliferation and cell death that may be important for therapy.

我们最初的假设可能仍然是正确的，但我们的重点在这一年中转向了更加公正的转录组学方法。我们对克隆 A 和 CX-1 的 7 天单层和球状培养物中表达的基因进行了全转录组分析。数据仍在评估中，但表明克隆 A 和 CX-1 球体中都有 462 个基因上调，其中克隆 A 中 249 个基因上调，但 CX-1 中下调，克隆 A 中 CX-1 中有 1,195 个基因上调，而 CX-1 中有 1,861 个基因上调。 CX-1 和克隆 A 中的基因下降。这些变化来自参考序列列表中总共超过 29,000 个基因，并添加了几个基因上次 refSeq 构建中不存在的重要基因亚型。 我们现在正在确定哪些途径受到的影响最大。我们的重点是识别可能对治疗很重要的细胞增殖和细胞死亡等途径。