Shape change and Nitric Oxide (NO) Modulation of Core Pluripotent TF Expression

核心多能 TF 表达的形状变化和一氧化氮 (NO) 调节

• 批准号: 8157692
• 负责人: John Jessup
• 金额: $ 5.51万
• 依托单位: DIVISION OF BASIC SCIENCES - NCI
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/8157692
• 关键词: Nitric Oxide; Shapes

Our initial postulate may still be correct but our focus shifted during the year to a more unbiased transcriptomic approach. We have performed whole transcriptomic analysis of the genes expressed in 7 day monolayer and spheroid cultures of Clone A and CX-1. The data are still under evaluation but suggest that there are 462 genes upregulated in both Clone A and CX-1 spheroids with 249 genes up in Clone A but down in CX-1 with 1,195 genes up in CX-1 down in Clone A and 1,861 genes down in CX-1 and Clone A. These changes are out of a total of more than 29,000 genes in the reference sequence list and the addition of several important isoforms of genes that are not present in last refSeq build. We are now in the process of determining what pathways are most affected. Our focus is on identification of pathways such as cell proliferation and cell death that may be important for therapy.

我们最初的假设可能仍然是正确的，但是我们的重点在一年中转变为一种更加偏见的转录组方法。我们已经对克隆A和CX-1的7天单层和球体培养物表达的基因进行了整个转录组分析。数据仍在评估中，但表明克隆A和CX-1球体中都有462个基因上调，在克隆A中有249个基因，但在CX-1中向下，但在CX-1中向下降低，在CX-1中，CX-1中的1,195个基因在克隆A和1,861个基因中的CX-1中向下下降，CX-1和1,861个基因在CX-1和CLONE A中，这些序列和克隆的变化是多数的序列。在最后一个refseq构建中不存在。 现在，我们正在确定哪种途径受到最大影响。我们的重点是鉴定可能对治疗很重要的细胞增殖和细胞死亡途径。