A Comprehensive Functional Map of Human Protein-RNA Interactions

人类蛋白质-RNA 相互作用的综合功能图谱

• 批准号: 10087955
• 负责人: Brenton R. Graveley
• 金额: $ 75.66万
• 依托单位: UNIVERSITY OF CONNECTICUT SCH OF MED/DNT
• 依托单位国家: 美国
• 财政年份: 2018
• 资助国家: 美国
• 起止时间: 2018-02-05 至 2022-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10087955
• 关键词: Affinity; Antibodies; Binding; Cell Line; Cells; ChIP-seq; Clustered Regularly Interspaced Short Palindromic Repeats; Code; Communities; Data; Data Analyses; Data Set; Databases; Disease; Elements; Encyclopedias; Engineering; Epitopes; Genetic; Genomics; Goals; HepG2; Human; Human Cell Line; Image; K-562; Language; Maps; Modality; Online Systems; Output; Process; Production; Property; Proteins; RNA; RNA Binding; RNA Databases; RNA-Binding Proteins; RNA-Protein Interaction; Reagent; Reproducibility; Resources; Scientist; Training; data portal; epigenomics; genome editing; genomic locus; insight; portability; stable cell line; tool; transcriptome sequencing; transcriptomics; user-friendly; vector; web portal

RESOURCE PROJECT - PROJECT SUMMARY The overall goal of ENCORE (Encyclopedia of RNA Elements) is to provide protein-RNA interaction maps for 300 RBPs in 2 human cell lines, and associated localization, binding motif and functional datasets that aid in interpretation of these data and assigning functions to the bulk of these RBPs. The goal of this resource project is to generate and validate stable cell lines expressing epitope-tagged RNA binding proteins, perform primary and integrated analysis of the human RBP physical and functional interaction data, and to develop publicly- accessible transcriptomic, binding affinity and subcellular localization databases. These three goals are critical to maximizing the impact of this resource by providing not only valuable physical resources to the community (e.g. tagged cell lines) but also integrated analysis of the data to obtain as much insight as possible into the functional and binding properties of each RBP. More importantly, making this data as accessible as possible to a broad community of scientists will be achieved by making all data available at public data portals such as the ENCODE DCC, as well as via our own web portals that host the datasets in formats amenable for web-based queries and focused browsing. We will also conduct training sessions in the use of these tools. Finally, we will facilitate reproducible data analysis by making all code we develop available using common workflow language that can be used on any compute server and platform.

资源项目 - 项目摘要 ENCORE（RNA 元素百科全书）的总体目标是提供蛋白质-RNA 相互作用图谱 2 个人类细胞系中的 300 个 RBP，以​​及相关的定位、结合基序和功能数据集，有助于 解释这些数据并将功能分配给大部分 RBP。该资源项目的目标 的目的是生成并验证表达表位标记的 RNA 结合蛋白的稳定细胞系，执行初级 和人类 RBP 物理和功能交互数据的综合分析，并开发公开- 可访问的转录组、结合亲和力和亚细胞定位数据库。这三个目标至关重要 通过不仅向社区提供宝贵的物质资源来最大限度地发挥该资源的影响 （例如标记的细胞系），还可以对数据进行综合分析，以尽可能多地了解 每个 RBP 的功能和结合特性。更重要的是，使这些数据尽可能易于访问 通过在公共数据门户（例如 ENCODE DCC，以及通过我们自己的门户网站以适合基于网络的格式托管数据集 查询和集中浏览。我们还将举办有关使用这些工具的培训课程。最后，我们将 通过使用通用工作流程语言提供我们开发的所有代码，促进可重复的数据分析 可以在任何计算服务器和平台上使用。