Molecular Biology

分子生物学

基本信息

批准号：
10078637
负责人：
Konstantin G Kousoulas
金额：
$ 25.82万
依托单位：
LOUISIANA STATE UNIV A&M COL BATON ROUGE
依托单位国家：
美国
项目类别：
财政年份：
2019
资助国家：
美国
起止时间：
2019-01-02 至 2023-12-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/10078637
关键词：
Acute Lung Injury Address Allergens Antibodies Biological Biological Assay Biology Biomedical Research Biotechnology Cells Cellular biology Centers of Research Excellence Computer software Consult Consultations Contract Services Core Facility Data Data Analyses Development Disease Electron Microscopy Endotoxins Ensure Equipment Experimental Designs Extrinsic asthma Faculty Faculty Recruitment Funding Future Gene Chips Gene Expression Gene Expression Profiling Generations Genomics Genotype Goals Human Human Genome Immunofluorescence Microscopy Immunologics Infection Inflammation Influenza Institution Kidney Laboratories Liver Louisiana Lung Lung diseases Maintenance Measures Mediating Mentors Methodology Methods Molecular Molecular Biology Molecular Biology Techniques Molecular Medicine Mus National Institute of General Medical Sciences Organ Pathogenesis Phase Plasmid Cloning Vector Preparation Productivity Proteins Proteomics Quantitative Reverse Transcriptase PCR Research Research Personnel Resources Reverse Transcriptase Polymerase Chain Reaction Sampling Sepsis Septic Shock Services Students Technical Expertise Technology Training Training and Education Tuberculosis United States National Institutes of Health Universities Update Veterinary Medicine Veterinary Schools Viral Vector assault bioimaging biomarker validation cecal ligation puncture experience immunopathology improved inflammatory lung disease instrumentation interest microbial mouse genome mouse model nano-string new technology repaired response single cell sequencing transcriptome sequencing transcriptomics

项目摘要

PROJECT SUMMARY The Molecular Biology (MB) Core has been created to meet the need for advanced molecular biology support by PJIs. In particular, access to and experience in utilizing advanced molecular biology that are limited for most PJIs and established NIH-funded investigators. Specifically, MB Core will leverage and expand the resources and expertise of the Genelab Core facility which is comprised of three existing Core Facilities operated by the Division of Biotechnology and Molecular Medicine (BioMMED) at the LSU School of Veterinary Medicine (SVM): Gene expression and sequencing Laboratory (GeneLab), Protein characterization and purification laboratory (PCPL), which is also responsible for generation of monospecific antibodies to proteins of interest, and the Molecular Immunopathology laboratory (MIPL) that provides expertise and access to bioimaging and advanced immunofluorescence and electron microscopy. These three laboratories are currently co-supported by the NIGMS-funded CEIDR-COBRE (Phase III) as well as the Louisiana Biomedical Research Network (LBRN/INBRE). The goal of the MB Core Facility of the Lung COBRE is to provide high quality instrumentation, sample preparation and training in a broad spectrum of fundamental and advanced molecular biology techniques to all COBRE PJIs and other NIH-funded investigators to achieve their research objectives. The MB Core has established 2 specific aims. Aim 1 is to provide the COBRE and other NIH-funded investigators with resources and expertise in molecular and cell biology, including instrumentation, technical support, consultation, and training. Aim 2 is to organize, coordinate, and facilitate interactions among COBRE investigators, mentors, and students, promote the development and use of new technologies and assays, and train PJI faculty, staff, and students in advanced molecular biology technologies.

项目概要分子生物学 (MB) 核心的创建是为了满足高级分子生物学支持的需求由 PJI 提供。特别是，对于大多数人来说，获得和利用先进分子生物学的经验是有限的。 PJI 和 NIH 资助的研究人员成立。具体来说，MB Core将利用和扩展资源 Genelab 核心设施的专业知识和专业知识，该设施由三个现有核心设施组成，由路易斯安那州立大学兽医学院生物技术和分子医学系 (BioMMED) (SVM)：基因表达和测序实验室(GeneLab)、蛋白质表征和纯化实验室（PCPL），还负责生成针对感兴趣蛋白质的单特异性抗体，分子免疫病理学实验室 (MIPL)，提供生物成像和生物成像方面的专业知识和途径先进的免疫荧光和电子显微镜。目前这三个实验室是共同支持的由 NIGMS 资助的 CEIDR-COBRE（第三阶段）以及路易斯安那生物医学研究网络（LBRN/INBRE）。 Lung COBRE 的 MB 核心设施的目标是提供高质量的仪器、广泛的基础和高级分子生物学的样品制备和培训向所有 COBRE PJI 和其他 NIH 资助的研究人员提供技术以实现他们的研究目标。 MB Core 制定了 2 个具体目标。目标 1 是为 COBRE 和其他 NIH 资助的研究人员提供分子和细胞生物学方面的资源和专业知识，包括仪器、技术支持、咨询、培训。目标 2 是组织、协调和促进 COBRE 之间的互动研究人员、导师和学生，促进新技术和检测的开发和使用，以及对 PJI 教职员工和学生进行先进分子生物学技术培训。