GeneLab Core

基因实验室核心

• 批准号: 8751060
• 负责人: Konstantin G Kousoulas
• 金额: $ 21.29万
• 依托单位: LOUISIANA STATE UNIV A&M COL BATON ROUGE
• 依托单位国家: 美国
• 财政年份: 2014
• 资助国家: 美国
• 起止时间: 2014-06-02 至 2019-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8751060
• 关键词: Achievement; Adopted; Applications Grants; Bacterial Genome; Bioinformatics; Biology; Biomedical Research; Biotechnology; Budgets; Centers of Research Excellence; Collaborations; Collection; Communities; Computer software; Consensus; Core Facility; Data; Data Analyses; Doctor of Philosophy; Ensure; Environment; Equipment; Faculty; Fee-for-Service Plans; Funding; Future; Gene Expression Profile; Generations; Grant; Growth; Human Resources; Ice; Imagery; Income; Individual; Infectious Diseases Research; Ions; Knowledge; Laboratories; Letters; Life; Louisiana; Methodology; Methods; Michigan; Molecular Biology; Molecular Medicine; National Institute of General Medical Sciences; Phase; Postdoctoral Fellow; Primates; Program Research Project Grants; Protocols documentation; Protons; Reagent; Reporting; Research; Research Infrastructure; Research Personnel; Research Project Grants; Resources; Scientist; Services; Source; System; Targeted Research; Technology; Training; Training Support; United States National Institutes of Health; Universities; Viral Genome; Wages; base; computer science; computerized tools; cost; digital; graduate student; new technology; next generation; next generation sequencing; pathogen; programs; undergraduate student

The GeneLab Core Facility provides molecular biology support, reagents and services to all Centerfor Experimental Infectious Disease Research (CEIDR) investigators. GeneLab sen/ices include general support and state-of-the art next generation sequencing (NGS) and bioinformatics support services. Of primary importance to all CEIDR faculty at Louisiana State University (LSU) and the Tulane National Primate Research Center (TNPRC) is the relatively new (one year) GeneLab state-of-the-art services, which are devoted to NGS and bioinformatics. These activities were started after consensus decisions were reached among CEIDR researchers that they were critically needed by ongoing NIH-funded research projects, as well as the entire Louisiana-based NIH-funded community. NGS equipment includes a Life Technologies, Inc. equipment suite with an Ion PGM and Ion Proton Sequencing Machines, as well as a Quant Studio Flex system equipped with digital PCR capabilities. GeneLab NGS activities are focused on confirmation and sequencing of viral and bacterial genomes, as well as the determination of pathogen and host transcriptional changes during host-pathogen interactions. Bioinformatics support services are provided through a collaboration with the Center for Computation and Technology (CCT), and the Louisiana Biomedical Research Network (LBRN), which is supported by the NIH: NIGMS INBRE grant P20GM103424. GeneLab is currently staffed with 5 PhD-level scientists, one research associate, two computer science graduate students and two undergraduate student workers. In addition, James Cavalcoli, PhD, Director of the Bionformatics Core at the University of Michigan, Ann Arbor serves as a consultant providing expert advice and support, as well as a resource for state-of-the art bioinformatics knowledge. The GeneLab Core fulfills a highly unmet need in the State of Louisiana, since there is no other similar support Core laboratory in the state.GeneLab's efforts in NGS and bioinformatics are consistent with the NIH Director's roadmap, which emphasizes computational approaches to biology and in our infectious disease research.

GeneLab 核心设施为所有中心提供分子生物学支持、试剂和服务 实验传染病研究 (CEIDR) 调查员。 GeneLab 服务包括一般 支持和最先进的下一代测序 (NGS) 和生物信息学支持服务。的 对路易斯安那州立大学 (LSU) 和杜兰国立大学的所有 CEIDR 教员来说至关重要 灵长类动物研究中心 (TNPRC) 是相对较新的（一年）GeneLab 最先进的服务， 致力于NGS和生物信息学。这些活动是在达成共识决定后开始的 CEIDR 研究人员一致认为，NIH 资助的正在进行的研究迫切需要他们 项目，以及整个路易斯安那州 NIH 资助的社区。 NGS 设备包括 Life Technologies, Inc. 设备套件，配有离子 PGM 和离子质子测序机，以及 Quant Studio Flex 系统配备数字 PCR 功能。 GeneLab NGS 活动的重点是 病毒和细菌基因组的确认和测序，以及病原体和病原体的确定 宿主与病原体相互作用期间宿主转录的变化。提供生物信息学支持服务 通过与计算与技术中心 (CCT) 和路易斯安那州 生物医学研究网络 (LBRN)，由 NIH: NIGMS INBRE 拨款 P20GM103424 支持。 GeneLab 目前拥有 5 名博士级科学家、1 名研究员、2 名计算机科学家 研究生和两名本科生工人。此外，詹姆斯·卡瓦尔科利 (James Cavalcoli) 博士， 密歇根大学安娜堡分校生物信息学核心担任顾问提供专家 建议和支持，以及最先进的生物信息学知识资源。基因实验室核心 满足路易斯安那州高度未满足的需求，因为没有其他类似的支持核心实验室 GeneLab 在 NGS 和生物信息学方面的努力与 NIH 主任的路线图一致， 它强调生物学和传染病研究中的计算方法。