ESTABLISHING A MUTANT MOUSE REGIONAL RESOURCE CENTER AT THE JACKSON LABORATORY

在杰克逊实验室建立突变小鼠区域资源中心

• 批准号: 8356886
• 负责人: LEAH RAE B DONAHUE
• 金额: $ 106.74万
• 依托单位: JACKSON LABORATORY
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-01-01 至 2011-12-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8356886
• 关键词: Affect; Animal Model; Archives; Bioinformatics; Biology; Biomedical Research; Communities; Coupled; Derivation procedure; Disease; Disease model; ES Cell Line; Explosion; Funding; Gene Targeting; Genes; Genetic; Genetically Engineered Mouse; Grant; Hereditary Disease; Human; Human Genetics; Life; Mus; Mutagenesis; Mutant Strains Mice; National Center for Research Resources; Pathology; Pharmaceutical Preparations; Phenotype; Play; Pluripotent Stem Cells; Prevention strategy; Principal Investigator; Production; Quality Control; Research; Research Infrastructure; Resources; Role; Safety; Scientist; Services; Source; System; Techniques; Technology; Testing; The Jackson Laboratory; Therapeutic; Transgenic Organisms; United States National Institutes of Health; biological systems; cost; experience; genome sequencing; human disease; induced pluripotent stem cell; mouse model; mutant; pre-clinical research; programs; public health relevance; repository; stem cell technology; virtual

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. DESCRIPTION (provided by applicant): This application proposes to establish a fourth Mutant Mouse Regional Resource Center (MMRRC) repository at The Jackson Laboratory (JAX). For over 60 years mutant mice, with phenotypes mimicking human diseases, have played a critical role in understanding the genetics underlying mammalian biology and human genetic disorders. The advent of transgenic and gene targeting technologies, coupled with large scale mutagenesis and gene trap programs has led to a virtual explosion in the production and use of mutant and genetically engineered mice. These valuable resources can only be fully exploited if they are archived, managed, and made accessible to the scientific community by centralized repositories. The Jackson Laboratory's experience and capacity as a repository, the quality of its existing programs, its management systems, and its reputation for research excellence all demonstrate that JAX will be a strong addition to the MMRRC network. In this application, we propose to establish a JAX MMRRC that would: 1) acquire mutant strains; 2) import and archive mutant strains; 3) distribute strains as cryopreserved material, mice recovered from cryopreserved material, ES cell lines, or live mice; 4) implement a state-of-the-art quality control program; 5) provide customer support and technical service; 6) promote MMRRC strains; 7) have an interactive Repository management plan; 8) establish the bioinformatics infrastructure to operate and to interact with other MMRRC repositories, and 9) conduct research to add utility to JAX MMRRC strains by developing pluripotent stem cell resources from valuable Repository disease models using both standard derivation techniques and IPS technology. The JAX MMRRC will be fully functional upon funding and will offer experience and capacity to the existing MMRRC program. PUBLIC HEALTH RELEVANCE (provided by applicant): The JAX Mutant Mouse Regional Resource Center (MMRRC) repository proposed in this project will provide access to mouse models for human disorders and for basic biomedical research to understand biological systems affected by human diseases. With a sequenced genome and increasingly sophisticated stem cell technologies to manipulate it, the mouse remains the most powerful model organism for biomedical research. Genetically engineered mouse models with homologous human genes are critical for translational and pre-clinical research. They enable scientists to understand the causes and pathologies of human diseases so that prevention strategies or therapeutics for these diseases can be developed. In addition, therapies or drugs can be tested on mouse models prior to human trials to assess efficacy and safety.

该副本是利用资源的众多研究子项目之一 由NIH/NCRR资助的中心赠款提供。对该子弹的主要支持 而且，副投影的主要研究员可能是其他来源提供的 包括其他NIH来源。 列出的总费用可能 代表subproject使用的中心基础架构的估计量， NCRR赠款不直接向子弹或副本人员提供的直接资金。 描述（由申请人提供）：本申请建议在杰克逊实验室（JAX）建立第四个突变的小鼠区域资源中心（MMRRC）存储库。 60多年来，具有模仿人类疾病的表型的突变小鼠在理解哺乳动物生物学和人类遗传疾病的基础遗传学方面发挥了关键作用。转基因和基因靶向技术的出现，加上大规模诱变和基因陷阱程序，导致了突变和基因工程小鼠的生产和使用中的虚拟爆炸。这些宝贵的资源只有在由集中式存储库对科学界的归档，管理和使科学界可以访问的情况下才能充分利用。杰克逊实验室作为存储库的经验和能力，其现有计划的质量，其管理系统的质量以及其卓越研究的声誉都表明，JAX将是MMRRC网络的强大补充。在此应用中，我们建议建立一个将：1）获得突变菌株的JAX MMRRC； 2）进口和存档突变株； 3）将菌株分配为冷冻材料，从冷冻保存材料，ES细胞系或活小鼠中回收的小鼠； 4）实施最先进的质量控制计划； 5）提供客户支持和技术服务； 6）促进MMRRC菌株； 7）有一个交互式存储库管理计划； 8）建立生物信息学基础架构以运行并与其他MMRRC存储库进行操作并与之相互作用，9）通过使用标准衍生技术和IPS技术从有价值的存储库模型中开发多能干细胞资源，从而对JAX MMRRC菌株进行研究，从而为JAX MMRRC菌株增加实用性。 JAX MMRRC将在资金时充分发挥作用，并将为现有MMRRC计划提供经验和能力。 公共卫生相关性（由申请人提供）：本项目提出的JAX突变小鼠区域资源中心（MMRRC）存储库将为人类疾病的小鼠模型提供访问和基本生物医学研究，以了解受人体疾病影响的生物系统。通过测序的基因组和越来越复杂的干细胞技术来操纵它，小鼠仍然是生物医学研究中最强大的模型生物。具有同源人类基因的基因工程小鼠模型对于翻译和临床前研究至关重要。它们使科学家能够理解人类疾病的原因和病理学，从而可以开发针对这些疾病的预防策略或治疗剂。此外，在人体试验之前，可以在小鼠模型上对疗法或药物进行测试，以评估功效和安全性。