The human temporal bone laboratory resource for basic and applied inner ear sciences

用于基础和应用内耳科学的人类颞骨实验室资源

基本信息

批准号：
10059242
负责人：
Akira Ishiyama
金额：
$ 61.86万
依托单位：
UNIVERSITY OF CALIFORNIA LOS ANGELES
依托单位国家：
美国
项目类别：
财政年份：
2016
资助国家：
美国
起止时间：
2016-12-01 至 2022-11-30
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/10059242
关键词：
Animal Model Animals Archives Auditory Automobile Driving Autopsy Basic Science Biological Biology Bone Tissue Celloidin Chicago Clinical Research Cochlea Collaborations Collection Communities Databases Detection Development Ear Edema Funding Future Goals Guidelines Harvest Histopathology Human Human Resources Image Immunofluorescence Immunologic Improve Access In Situ In Situ Hybridization Institution Instruction Laboratories Labyrinth Liquid substance Magnetic Resonance Imaging Maintenance Medical Students Medical center Messenger RNA Methodology Methods Microdissection Modernization Molecular National Institute on Deafness and Other Communication Disorders Otolaryngology Pathology Pharmacotherapy Preparation Process Production Proteins Proteomics Protocols documentation Registries Research Research Personnel Research Proposals Resolution Resources Scanning Electron Microscopy Science Scientist Site Specimen Specimen Handling Stains Structure Techniques Temporal bone structure Time Tissue Banks Tissue membrane Tissues Training Transmission Electron Microscopy Universities Update Visualization biological specimen archives bone imaging cost graduate student human tissue improved in vivo interest medical schools middle ear new technology preservation repository skills tissue archive tissue preparation tool translational study transmission process

项目摘要

ABSTRACT In the present proposal we will preserve, process and make available human ear tissue specimens for inner ear basic science researchers such that they may be able to compare with their animal models. The use of celloidin and microdissected human temporal bone tissue for immunohistochemical and quantitative techniques will be instrumental in driving basic animal inner ear research forward towards understanding of human ear pathology and has high potential for the development of effective drug therapies for restoring auditory and vestibular function. In Specific Aim 1) we will improve access for the auditory and vestibular research community to human ear tissues from temporal bone specimens. We will improve and promote timely procurement, preparing and processing of new specimens, and appropriate re-processing of archived specimens. We will distribute a limited number of well-prepared laboratory tissues to funded researchers who lack adequate access to facilities for human temporal bone preparation. We will also exploit use of the Registry for information about currently archived tissues and their access by the community, and promote collaborations between researchers seeking to validate animal models for human relevance. Specific Aim 2) We will optimize tissue preparation techniques. We will include molecular and cellular marking and visualization techniques that allow direct comparisons of fresh and archived human tissues with those from animal models. We will disseminate protocols for procurement, preservation and preparation of human ear tissues from temporal bones. By doing this we will encourage animal researchers to include human tissues into their analyses. Specific Aim 3) We will use high resolution MRI to evaluate the fluid-filled inner ear structures, and ex vivo MRI to evaluate the inner ear structures post-mortem and make comparisons with celloidin- embedded histopathology. We will exploit new technologies and methods to image and visualize human middle and inner ears in situ with the aim of visualizing soft cellular tissues, membranes and fluids, as well as hard bony tissues. Specific Aim 4) We will provide technical instruction with hands-on training will be available for investigators, technicians otolaryngology residents, medical school and graduate students interested in inner ear biology in the temporal bone processing techniques and skills needed for processing human ear tissues from temporal bones. The materials generate in this proposal will be mutually beneficial for researchers in basic scientific studies of the inner ear, as well as to temporal bone scientists wishing to apply high levels of molecular and cellular techniques to the human temporal bone. These collaborative efforts hold promise to rapidly accelerate the science of temporal bone processing and the understanding of human otopathologies.

抽象的在目前的提案中，我们将保存、处理和提供人耳组织样本，用于内部研究耳朵基础科学研究人员，以便他们能够与他们的动物模型进行比较。使用赛璐珞和显微解剖的人颞骨组织用于免疫组织化学和定量技术将有助于推动基础动物内耳研究的发展，以了解人耳病理学，具有开发有效药物疗法以恢复听力的巨大潜力听觉和前庭功能。在具体目标 1) 中，我们将改善听觉和前庭的通道研究界从颞骨标本中提取人耳组织。我们将及时改进和推广采购、准备和处理新样本，以及对存档的样本进行适当的再处理标本。我们将向受资助的研究人员分发有限数量的准备充分的实验室组织缺乏足够的人颞骨制备设施。我们还将利用登记当前存档组织的信息及其社区的访问权限，并促进研究人员之间的合作，旨在验证动物模型与人类的相关性。具体目标 2) 我们将优化组织制备技术。我们将包括分子和细胞标记以及可视化技术，可以将新鲜和存档的人体组织与来自的组织进行直接比较动物模型。我们将传播人耳采购、保存和准备的协议来自颞骨的组织。通过这样做，我们将鼓励动物研究人员将人体组织纳入其中他们的分析。具体目标 3) 我们将使用高分辨率 MRI 来评估充满液体的内耳结构，和离体 MRI 来评估死后内耳结构并与赛璐珞进行比较嵌入式组织病理学。我们将利用新技术和新方法对人类进行成像和可视化中耳和内耳原位观察，目的是观察软细胞组织、膜和液体，以及硬骨组织。具体目标 4) 我们将提供技术指导，并提供实践培训适合对以下领域感兴趣的研究人员、技术人员、耳鼻喉科住院医师、医学院和研究生内耳生物学中的颞骨处理技术和处理人耳所需的技能来自颞骨的组织。本提案中生成的材料将为研究人员带来互惠互利内耳基础科学研究以及希望应用高水平的颞骨科学家分子和细胞技术对人类颞骨的影响。这些合作努力有望迅速加速颞骨加工科学和对人类耳病理学的理解。