The Human Temporal Bone Laboratory Resource for Basic and Applied Inner Ear Sciences

基础和应用内耳科学的人类颞骨实验室资源

基本信息

批准号：
10457741
负责人：
Akira Ishiyama
金额：
$ 62.67万
依托单位：
UNIVERSITY OF CALIFORNIA LOS ANGELES
依托单位国家：
美国
项目类别：
财政年份：
2021
资助国家：
美国
起止时间：
2021-07-28 至 2022-11-30
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/10457741
关键词：
Animal Model Animals Archives Auditory Automobile Driving Autopsy Basic Science Biological Biology Celloidin Cells Clinic Clinical Research Collaborations Collection Communities Confocal Microscopy Detection Development Ear Equilibrium Equipment and supply inventories Functional disorder Funding Future Guidelines Hearing Hearing problem Hematoxylin and Eosin Staining Method Human Image Imaging Techniques Immunofluorescence Immunologic Immunohistochemistry Improve Access In Situ Instruction Laboratories Labyrinth Lead Life Liquid substance Medical Students Messenger RNA Methodology Methods Modernization Molecular National Institute on Deafness and Other Communication Disorders Otolaryngology Pathologic Pathology Pharmacotherapy Postmortem Changes Preparation Process Prospective Studies Proteins Proteomics Protocols documentation Registries Research Research Personnel Research Proposals Resolution Resources Science Scientist Specimen Specimen Handling Stains Techniques Temporal bone structure Time Tissue Banks Tissue membrane Tissues Training Transmission Electron Microscopy Vestibular Diseases Visualization base biological specimen archives design graduate student human tissue improved interest medical schools method development middle ear new technology non-invasive imaging preservation prevent protein expression skills targeted imaging tissue archive tissue preparation tool translational study

项目摘要

ABSTRACT In the present proposal we will preserve, process and make available human ear tissue to inner ear basic researchers they will be able to compare with their animal models. The use of celloidin, microdissected tissue for immunohistochemical and quantitative techniques will be instrumental in driving basic animal inner ear research into the understanding of human ear pathology and has high potential for the development of effective drug therapies for restoring auditory and vestibular function. In Specific Aim 1) we will improve access for the auditory and vestibular research community to human ear tissues from temporal bone specimens. We will improve and promote timely procurement, preparing and processing of new specimens, and appropriate re- processing of archived specimens. We will distribute a limited number of well-prepared laboratory tissues to funded researchers who lack adequate access to facilities for human temporal bone preparation. We will also exploit use of the Registry for information about currently archived tissues and their access by the community, and promote collaborations between researchers seeking to validate animal models for human relevance. Specific Aim 2) We will optimize tissue preparation techniques. We will include molecular and cellular marking and visualization techniques that allow direct comparisons of fresh and archived human tissues with those from animal models. We will disseminate protocols for procurement, preservation and preparation of human ear tissues from temporal bones. By doing this we will encourage animal researchers to include human tissues into their analyses. Specific Aim 3) We will develop tools to exploit new technologies and methods to image and visualize human middle and inner ears in situ. Targets for imaging techniques will include soft cellular tissues, membranes and fluids, as well as hard bony tissues. Specific Aim 4) We will provide technical instruction with hands-on training will be available for investigators, technicians otolaryngology residents, medical school and graduate students interested in inner ear biology in the temporal bone processing techniques and skills needed for processing human ear tissues from temporal bones. The materials generate in this proposal will be extremely useful for the research in basis inner ear research field, in the USA and around the globe.

抽象的在本提案中，我们将保存、处理人耳组织并将其提供给内耳基础研究人员将能够与他们的动物模型进行比较。使用赛璐珞、显微解剖组织免疫组织化学和定量技术将有助于驱动基本的动物内耳研究了解人耳病理学，并具有开发有效治疗方法的巨大潜力用于恢复听觉和前庭功能的药物治疗。在具体目标 1) 中，我们将改善听觉和前庭研究界从颞骨标本中提取人耳组织。我们将改进和促进新标本的及时采购、准备和处理，以及适当的再处理存档标本的处理。我们将分发数量有限的精心准备的实验室组织资助缺乏足够的人类颞骨制备设施的研究人员。我们也会利用登记处获取有关当前存档的组织及其社区访问的信息，并促进研究人员之间的合作，以验证动物模型与人类的相关性。具体目标 2) 我们将优化组织制备技术。我们将包括分子和细胞标记以及可视化技术，可以将新鲜和存档的人体组织与来自的组织进行直接比较动物模型。我们将传播人耳采购、保存和准备的协议来自颞骨的组织。通过这样做，我们将鼓励动物研究人员将人体组织纳入其中他们的分析。具体目标 3) 我们将开发工具来利用新技术和方法来成像和现场可视化人类中耳和内耳。成像技术的目标将包括软细胞组织、膜和液体，以及硬骨组织。具体目标 4) 我们将提供技术指导将为调查人员、耳鼻喉科住院医师、医学院和技术人员提供实践培训对内耳生物学感兴趣的研究生在颞骨处理技术和所需技能方面用于处理来自颞骨的人耳组织。该提案中生成的材料将非常对于美国和全球范围内基础内耳研究领域的研究很有用。

项目成果

期刊论文数量（32）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

Mutation of SLC7A14 causes auditory neuropathy and retinitis pigmentosa mediated by lysosomal dysfunction.

SLC7A14 突变会导致溶酶体功能障碍介导的听神经病变和色素性视网膜炎。

DOI：
发表时间：
2022-04-08
期刊：
影响因子：
13.6
作者：
Giffen, Kimberlee P;Li, Yi;Liu, Huizhan;Zhao, Xiao;Zhang, Chang;Shen, Ren;Wang, Tianying;Janesick, Amanda;Chen, Bo;Gong, Shu;Kachar, Bechara;Jin, Zi;He, David Z
通讯作者：
He, David Z

Cochlear Ossification After Vestibular Schwannoma Surgery: A Temporal Bone Study.

前庭神经鞘瘤手术后的耳蜗骨化：颞骨研究。

DOI：
发表时间：
2023-08
期刊：
影响因子：
0
作者：
Itamura, Kyohei;Geerardyn, Alexander;Podury, Archana;Lopez, Ivan A;Wong, Yu;Ishiyama, Akira;Quesnel, Alicia M;Miller, Mia E
通讯作者：
Miller, Mia E

Endolymphatic Hydrops in the Setting of Vestibular Schwannoma: A Temporal Bone Study.

前庭神经鞘瘤中的内淋巴积水：颞骨研究。