Core D - Immunopathology

核心 D - 免疫病理学

基本信息

批准号：
10023667
负责人：
GEORGE F MURPHY
金额：
$ 17.45万
依托单位：
UNIVERSITY OF PITTSBURGH AT PITTSBURGH
依托单位国家：
美国
项目类别：
财政年份：
2015
资助国家：
美国
起止时间：
2015-05-15 至 2025-07-31
项目状态：
未结题

来源：
https://reporter.nih.gov/project-details/10023667
关键词：
Affect Algorithmic Analysis Antibodies Archives Autoimmune Diseases Autoimmunity BCL2 gene Biological Specimen Banks Body Weight decreased CASP3 gene CD8B1 gene Cell physiology Cells Chronic Classification Cleaved cell Clinical Communities Complement Computer software Data Databases Detection Development Epitopes Evaluation FOXP3 gene Feedback Formalin Funding Future Genes Goals Histologic Histology ITGAM gene ITGAX gene Image Image Analysis Immunity Immunofluorescence Immunologic Immunologics Individual Investigation Label Learning Lesion Letters Libraries Machine Learning Maintenance Malignant Neoplasms Mediator of activation protein Molecular Morphology Mus Mutant Strains Mice NCAM1 gene PTPRC gene Paraffin Embedding Pathology Pathway interactions Periodicity Positioning Attribute Protocols documentation Research Personnel Resolution Sampling Scanning Severities Site Slide Stains Standardization T-Lymphocyte Technology Tissue Sample Tissues Tumor Immunity Update Virus Diseases Visualization Woman Work antiviral immunity base chronic infection congenic deep learning design gene product immunopathology meetings multiplexed imaging prevent programmed cell death protein 1 programs prospective quantitative imaging receptor success synergism

项目摘要

CORE SUMMARY The overall goal of this PPG application is to develop a mechanistic understanding of the cellular and molecular basis for PD1 and LAG3 interactions that regulate T cell function in autoimmunity, cancer and chronic viral infections. Core D will provide sophisticated and relevant technologies and expertise to PPG investigators for understanding how the interplay between PD1 and LAG3 affects cellular interactions and relevant molecular pathways in intact experimental tissues from mice with autoimmune disease (Project 1), cancer (Project 2) and chronic viral infection (Project 3). The Aims of this Core are: AIM 1: To provide basic expertise and technologies for detailed immunopathologic tissue analysis; AIM 2: To apply high- resolution quantitative, correlative immunopathology using highly-multiplexed cyclic immunofluorescence (t- CyCIF); and AIM 3: To maintain an annotated biospecimen bank/archive for future studies. These data will interface with data generated by Core C and PPG projects in order to develop protocols to visualize new molecules, as well as metrics generated from conventional histology (e.g., qualitative assessment and grading of lesion severity) and clinical parameters (e.g. survival and weight loss). Core D will interact extensively with all three Projects, with Core A for oversight and administrative support, and Core C by developing new staining protocols for cellular and histologic investigation of key genes and their products that Core C determines are regulated by PD1 and LAG3.

核心摘要该 PPG 应用程序的总体目标是建立对细胞和细胞的机制理解。 PD1 和 LAG3 相互作用调节自身免疫、癌症和慢性病中 T 细胞功能的分子基础病毒感染。 Core D 将为 PPG 调查人员提供先进且相关的技术和专业知识了解 PD1 和 LAG3 之间的相互作用如何影响细胞相互作用以及患有自身免疫性疾病的小鼠完整实验组织中的相关分子途径（项目 1）、癌症（项目 2）和慢性病毒感染（项目 3）。该核心的目标是：目标 1：提供详细的免疫病理组织分析的基本专业知识和技术；目标2：应用高使用高度多重循环免疫荧光（t- CYCIF）；目标 3：维护带注释的生物样本库/档案以供未来研究使用。这些数据将与 Core C 和 PPG 项目生成的数据接口，以开发协议来可视化新的分子，以及传统组织学产生的指标（例如，定性评估和分级病变严重程度）和临床参数（例如生存率和体重减轻）。 Core D 将与所有三个项目，核心 A 负责监督和行政支持，核心 C 负责开发新染色 Core C 确定的关键基因及其产物的细胞和组织学研究方案是受 PD1 和 LAG3 调节。