Small non-coding RNAs regulate retinal ganglion cell maturation and the developmental loss of intrinsic axon growth capacity

小非编码 RNA 调节视网膜神经节细胞成熟和内在轴突生长能力的发育丧失

基本信息

批准号：
10004833
负责人：
Feliks Ephraim Trakhtenberg
金额：
$ 3.99万
依托单位：
UNIVERSITY OF CONNECTICUT SCH OF MED/DNT
依托单位国家：
美国
项目类别：
财政年份：
2019
资助国家：
美国
起止时间：
2019-06-01 至 2024-05-31
项目状态：
已结题

项目摘要

Project Summary The retinal ganglion cells convey information from the eye to the brain through their axons in the optic nerve and the optic tract. The molecular mechanisms of retinal ganglion cell axon growth are still poorly understood, and adult retinal ganglion cells do not spontaneously regenerate injured axons, contributing to the loss of vision associated with optic nerve trauma and optic neuropathies. Our goal is to investigate a novel approach for stimulating the retinal ganglion cells’ intrinsic capacity to regenerate axons through the optic nerve to restore visual responses. We will utilize established animal models for investigating the roles of small non-coding RNAs in regulating retinal ganglion cell maturation and the developmental loss of axon growth capacity, and test their potential for regenerating the injured optic nerve. We will also investigate through which molecular pathways the identified small non-coding RNAs regulate neurite growth. Furthermore, we will investigate the roles of these small non-coding RNAs in arborization of retinal ganglion cell dendrites during retinal development. We expect that these studies will advance our understanding of the roles the small non-coding RNAs play in retinal ganglion cell biology, as well as potentially lead to the development of novel approaches for restoring simple visual functions after optic nerve injury.

项目概要视网膜神经节细胞通过视神经中的轴突将信息从眼睛传递到大脑，视网膜神经节细胞轴突生长的分子机制仍知之甚少，并且成人视网膜神经节细胞不会自发再生受损的轴突，从而导致视力丧失我们的目标是研究一种与视神经损伤和视神经病变相关的新方法。刺激视网膜神经节细胞通过视神经再生轴突的内在能力，以恢复我们将利用已建立的动物模型来研究小非编码 RNA 的作用。调节视网膜神经节细胞成熟和轴突生长能力的发育丧失，并测试它们的作用我们还将研究通过哪些分子途径再生受损的视神经。鉴定出的小非编码 RNA 调节神经突生长此外，我们将研究它们的作用。我们预计视网膜发育过程中视网膜神经节细胞树突的树枝状化中存在小非编码RNA。这些研究将增进我们对小非编码 RNA 在视网膜神经节中所起作用的理解细胞生物学，并有可能导致恢复简单视觉的新方法的开发视神经损伤后的功能。