Next-Generation Genomic Imaging Technology

下一代基因组成像技术

基本信息

批准号：
10026446
负责人：
Tushar Jasubhai DESAI
金额：
$ 39.47万
依托单位：
STANFORD UNIVERSITY
依托单位国家：
美国
项目类别：
财政年份：
2018
资助国家：
美国
起止时间：
2018-09-15 至 2022-06-30
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/10026446
关键词：
3-Dimensional Adopted Adoption Anatomy Antibodies Atlases Benchmarking Biology Biomedical Research Bypass Cells Collaborations Data Detection Development Disease Energy Transfer Equipment and supply inventories Fluorescence Fluorescence Microscopy Genomics Hour Human Image Imaging technology In Situ Individual Label Lanthanoid Series Elements Libraries Ligands Light Liver Logic Lung Maps Marriage Mass Spectrum Analysis Microscope Molecular Molecular Analysis Mus Noise Oligonucleotides Optics Organ Pattern Penetration Performance Physiological Positioning Attribute Property Reagent Research Research Personnel Resolution Scientist Sensitivity and Specificity Signal Transduction Stains Structure Techniques Technology Testing Thick Tissue Sample Tissues aqueous base cell type cost data acquisition design fluorescence imaging fluorophore human tissue luminescence mass spectrometer microscopic imaging millimeter molecular imaging new technology next generation novel passive transport receptor reconstruction serial imaging single cell sequencing tool

项目摘要

Single cell genomics has transformed biology by enabling unbiased molecular identification and characterization of the full panoply of cell types that make up a tissue. A 'marriage' between this molecular information, which includes an inventory of all the ligands and receptors expressed by each cell class, and the accompanying 3D spatial information about them will enable the comprehensive reconstruction of the active signaling centers within a given tissue. Here, by continuing a fruitful collaboration between a biologist and molecular biochemist, we propose to deliver a transformative new technology for 3D multi-dimensional molecular imaging in intact human tissue. A major strength of our approach is that we entirely bypass the intrinsic limitations of fluorescent imaging, namely endogenous autofluorescence, low signal to noise, and restriction to five or fewer data channels, by using lanthanide resonant energy transfer. By so doing, we will simultaneously overcome challenges in detection of individual signals and massively increase throughput. Furthermore, the technology we deliver will be both biologist 'friendly' and low cost, providing a widely available and practical platform that will be rapidly and broadly adopted by scientists worldwide.

单细胞基因组学通过实现公正的分子生物学改变了生物学构成细胞的全部细胞类型的鉴定和表征组织。这种分子信息之间的“联姻”，其中包括每个细胞类别表达的所有配体和受体的清单，以及附带的有关它们的 3D 空间信息将使全面的重建给定组织内的活性信号中心。在这里，由继续生物学家和分子生物化学家之间富有成效的合作，我们提议为 3D 多维提供革命性的新技术完整人体组织的分子成像。我们方法的一个主要优势是我们完全绕过了荧光成像的内在局限性，即内源自发荧光、低信噪比、限制为 5 个或更少数据通道，通过使用镧系元素共振能量传输。通过这样做，我们将同时克服检测单个信号的挑战大幅提高吞吐量。此外，我们提供的技术将是生物学家“友好”且成本低廉，提供了广泛可用且实用的该平台将被全世界科学家迅速广泛采用。