CHARACTERIZATION OF ANTIGENIC PEPTIDES PRESENTED BY I-AG7

I-AG7 呈现的​​抗原肽的表征

• 批准号: 8168793
• 负责人: EMIL Raphael UNANUE
• 金额: $ 0.62万
• 依托单位: WASHINGTON UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-03-10 至 2010-12-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8168793
• 关键词: Cataloging; Catalogs; Computer Retrieval of Information on Scientific Projects Database; Funding; Goals; Grant; Histocompatibility Antigens Class II; Inbred NOD Mice; Institution; Mass Spectrum Analysis; Mouse Cell Line; Peptides; Post-Translational Protein Processing; Proteins; Research; Research Personnel; Resources; Source; United States National Institutes of Health; improved; insulinoma; technique development

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The goal of this project is to identify peptides presented by the diabetogenic MHC class II molecule I-Ag7 from NOD mice. Studies will use both NOD mice and cell lines derived from insulinomas. The study will extend to cover the effect of loading, posttranslational modification, and challenge by foreign peptides and proteins. Mass spectrometry will be used to sequence and catalog these peptides and the development of techniques to improve sensitivity and analysis will be pursued.

该副本是利用众多研究子项目之一 由NIH/NCRR资助的中心赠款提供的资源。子弹和 调查员（PI）可能已经从其他NIH来源获得了主要资金， 因此可以在其他清晰的条目中代表。列出的机构是 对于中心，这不一定是调查员的机构。 该项目的目的是确定来自NOD小鼠的糖尿病性MHC II类I-AG7所呈现的肽。 研究将同时使用来自胰岛素瘤的小鼠和细胞系。 该研究将扩展以涵盖外肽和蛋白质的负载，翻译后修饰和挑战的效果。 质谱法将用于对这些肽进行测序和分类，并将采用提高灵敏度和分析的技术的发展。