RESOURCE FOR NONHUMAN PRIMATE IMMUNE REAGENTS

非人灵长类免疫试剂资源

• 批准号: 8172314
• 负责人: Francois J Villinger
• 金额: $ 5.48万
• 依托单位: EMORY UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-05-01 至 2011-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8172314
• 关键词: Biological; CD4 Positive T Lymphocytes; Calendar; Cataloging; Catalogs; Cells; Cercocebus atys; Code; Complementary DNA; Computer Retrieval of Information on Scientific Projects Database; Drug Kinetics; Funding; Grant; Granulocyte-Macrophage Colony-Stimulating Factor; IL12B gene; Immune; Immune response; Institution; Interferon Type II; Interleukin-15; Interleukin-17; Interleukin-7; Macaca mulatta; Modeling; Outcome; Potassium Channel Blockers; Production; Reagent; Recombinant Proteins; Research; Research Personnel; Resources; SIV; Source; Spliced Genes; TNFSF4 gene; Testing; United States National Institutes of Health; Variant; Viral; cytokine; expression cloning; in vivo; interleukin-23; nonhuman primate

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. This project serves as a resource for the testing, optimization of use and distribution of nonhuman primate specific immune related reagents. Therefore, cDNA coding for various nonhuman primate cytokines have been and continue to be cloned and sequenced. During the past year, the genes and splice variants for PD-1, PD-L1, PD-L2, OX40L, MIP3a, IL-17, IL-23, 4-1BBL, CD24 and IL-15Ra have been added to the catalogue of Old-World NHP cDNAs available. In addition, the production of select nonhuman primate recombinant proteins such as rMamu RANTES, IFN-a, IFN-g, GM-CSF, soluble PD1, IL-7, 10 and 15 has been ongoing. Within the calendar year, the Resource has provided a total of 54 reagents to 20 investigators. These reagents included 33 cDNA or expression clones, 23 orders of cytokine recombinant proteins in varying amounts and 1 order of transfected cell producing rMamu IL-12B. Several additional studies were done in part or fully by this resource: The pharmacokinetics and biological activity of select potassium channels blockers Pap-1, Shk(l)5 and TRAM-34 were tested in vivo. The administration of IL-15 and IL-7 in uninfected and SIV infected rhesus macaques was tested for the delineation of these cytokine's influence on viral replication and immune responses in vivo. In addition, this resource has tested the depletion of CD4+ T cells from sooty mangabeys to study the immunological outcome of such depletion in vivo, as well as the potential benefit of rIL-7 therapy in restoring the CD4 compartment in this model.

该副本是利用众多研究子项目之一 由NIH/NCRR资助的中心赠款提供的资源。子弹和 调查员（PI）可能已经从其他NIH来源获得了主要资金， 因此可以在其他清晰的条目中代表。列出的机构是 对于中心，这不一定是调查员的机构。 该项目是测试，优化使用和分布非人类灵长类动物特定免疫相关试剂的资源。因此，编码各种非人类灵长类动物细胞因子的cDNA已经并继续被克隆和测序。在过去的一年中，PD-1，PD-L1，PD-L2，OX40L，MIP3A，IL-17，IL-17，IL-23、4-1BBL，CD24和IL-15RA的基因和剪接变体已添加到可用的老World NHP CDNA的目录中。此外，生产精选的非人类灵长类动物重组蛋白，例如RMAMU RANTES，IFN-A，IFN-G，GM-CSF，可溶性PD1，IL-7、10和15。 在日历年内，该资源为20名调查人员提供了54种试剂。这些试剂包括33个cDNA或表达克隆，有23个以不同量的细胞因子重组蛋白和1个产生RMAMU IL-12B的转染细胞的顺序。该资源部分或全面完成了一些其他研究：在体内测试了精选钾通道的药代动力学和生物学活性PAP-1，SHK（L）5和TRAM-34。 测试了这些细胞因子对体内病毒复制和免疫反应的影响，测试了未感染和SIV感染的恒河猕猴中IL-15和IL-7的给药。 此外，该资源已经测试了从烟草芒果的CD4+ T细胞的耗竭，以研究这种耗尽体内这种耗竭的免疫学结果，以及RIL-7治疗在恢复该模型中CD4腔中的潜在益处。