MEDICAL STRUCTURAL GENOMICS OF PATHOGENIC PROTOZOA

致病原虫的医学结构基因组学

• 批准号: 8170019
• 负责人: ETHAN A MERRITT
• 金额: $ 0.51万
• 依托单位: STANFORD UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-05-01 至 2011-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8170019
• 关键词: Active Sites; African Trypanosomiasis; Binding; Chagas Disease; Chemicals; Computer Retrieval of Information on Scientific Projects Database; Funding; Grant; Institution; L-Selenomethionine; Leishmania; Leishmaniasis; Medical; Phase; Plasmodium; Proteins; Protozoa; Research; Research Personnel; Resources; Robotics; Screening procedure; Source; Structure; Trypanosoma; Trypanosoma cruzi; United States National Institutes of Health; follow-up; pathogen; small molecule; structural genomics

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. This proposal encompasses the initial structure determination of medically relevant target proteins from the eukaryotic pathogens Trypanosoma bruci (African sleeping sickness). T. cruzi (Chagas disease), Leishmania spp. (leishmaniasis) and Plasmodium spp. (maleria), and follow-up screening of small molecule cocktails to identify chemical fragments that bind at the active site. Initial structure determinations will rely primarily on SeMet MAD/SAD phasing. Screening of cocktail soaks can be done at fixed wavelength, but benifits greatly from the use of robotic automounting and screening.

该副本是利用众多研究子项目之一 由NIH/NCRR资助的中心赠款提供的资源。子弹和 调查员（PI）可能已经从其他NIH来源获得了主要资金， 因此可以在其他清晰的条目中代表。列出的机构是 对于中心，这不一定是调查员的机构。 该提案涵盖了来自真核病原体锥虫瘤（非洲睡眠疾病）的医学相关靶蛋白的初始结构测定。 T. Cruzi（Chagas病），Leishmania spp。 （利什曼病）和疟原虫。 （Maleria），以及对小分子鸡尾酒的后续筛选，以鉴定在活性位点结合的化学片段。初始结构确定将主要依赖于SEMET MAD/SAD相位。 可以在固定的波长下进行鸡尾酒浸泡的筛选，但由于使用机器人自动启动和筛选而大大促进了鸡尾酒浸泡。