MEDICAL STRUCTURAL GENOMICS OF PATHOGENIC PROTOZOA

致病原虫的医学结构基因组学

• 批准号: 8362112
• 负责人: ETHAN A MERRITT
• 金额: $ 0.22万
• 依托单位: STANFORD UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-03-01 至 2012-02-29
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8362112
• 关键词: Active Sites; African Trypanosomiasis; Binding; Chagas Disease; Chemicals; Funding; Grant; L-Selenomethionine; Leishmania; Leishmaniasis; Medical; National Center for Research Resources; Phase; Plasmodium; Principal Investigator; Proteins; Protozoa; Radiation; Research; Research Infrastructure; Resources; Robotics; Screening procedure; Source; Structure; Trypanosoma; Trypanosoma cruzi; United States National Institutes of Health; cost; follow-up; pathogen; small molecule; structural biology; structural genomics

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. This proposal encompasses the initial structure determination of medically relevant target proteins from the eukaryotic pathogens Trypanosoma bruci (African sleeping sickness). T. cruzi (Chagas disease), Leishmania spp. (leishmaniasis) and Plasmodium spp. (maleria), and follow-up screening of small molecule cocktails to identify chemical fragments that bind at the active site. Initial structure determinations will rely primarily on SeMet MAD/SAD phasing. Screening of cocktail soaks can be done at fixed wavelength, but benifits greatly from the use of robotic automounting and screening.

该子项目是利用资源的众多研究子项目之一 由 NIH/NCRR 资助的中心拨款提供。子项目的主要支持 并且子项目的首席研究员可能是由其他来源提供的， 包括其他 NIH 来源。 子项目可能列出的总成本 代表子项目使用的中心基础设施的估计数量， NCRR 赠款不直接向子项目或子项目工作人员提供资金。 该提案包括对来自真核病原体布氏锥虫（非洲昏睡病）的医学相关目标蛋白进行初步结构测定。克氏锥虫（查加斯病）、利什曼原虫属。 （利什曼病）和疟原虫属。 （疟疾），并对小分子混合物进行后续筛选，以识别在活性位点结合的化学片段。初始结构测定将主要依赖于 SeMet MAD/SAD 定相。 鸡尾酒浸泡的筛选可以在固定波长下完成，但使用机器人自动安装和筛选可以大大受益。