CHARACTERIZATION OF THE ROLE OF METAL IONS IN RNA FOLDING AND ACTIVATION

金属离子在 RNA 折叠和激活中的作用表征

• 批准号: 8170130
• 负责人: James E. Penner-Hahn
• 金额: $ 0.2万
• 依托单位: STANFORD UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-05-01 至 2011-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8170130
• 关键词: Adopted; Complex; Computer Retrieval of Information on Scientific Projects Database; DNA; Divalent Cations; Funding; Grant; Institution; Ions; Label; Metal Binding Site; Metals; Nature; Play; RNA; Research; Research Personnel; Resources; Role; Source; Structure; Testing; United States National Institutes of Health; Vertebral column; inorganic phosphate

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Divalent cations play a critical role in the folding and activation of RNA. Unlike DNA, where the canonical structure is well defined, RNAs are found to adopt a wide range of structures. In many cases, divalent cations (Mg2+, Mn2+, and Zn2+) are required for the RNA to adopt a biologically active structure. Recent NMR studies have provided direct evidence for a unique binding site of the metal to the RNA and have suggested that the metal forms an inner-sphere complex with the RNA. EXAFS will be used to test this proposal and to better define the nature of the RNA-metal interaction. In order to define specifically the RNA-metal binding site, thio-modified RNA will be used to label possible backbone phosphates.

该副本是利用众多研究子项目之一 由NIH/NCRR资助的中心赠款提供的资源。子弹和 调查员（PI）可能已经从其他NIH来源获得了主要资金， 因此可以在其他清晰的条目中代表。列出的机构是 对于中心，这不一定是调查员的机构。 二价阳离子在RNA的折叠和激活中起关键作用。与DNA不同的是，在典范结构中定义了很好的定义，发现RNA采用了广泛的结构。在许多情况下，RNA采用生物学活性结构需要二价阳离子（Mg2+，Mn2+和Zn2+）。最近的NMR研究为金属与RNA的独特结合位点提供了直接的证据，并提出金属与RNA形成了内球体复合物。 EXAF将用于测试该提案，并更好地定义RNA - 金属相互作用的性质。为了特别定义RNA - 金属结合位点，将使用硫硫化的RNA来标记可能的骨链磷酸盐。