Enhanced Development of the Xiphophorus Model System

剑龙模型系统的增强发展

• 批准号: 7817115
• 负责人: RONALD B WALTER
• 金额: $ 43万
• 依托单位: TEXAS STATE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2008
• 资助国家: 美国
• 起止时间: 2008-04-01 至 2011-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7817115
• 关键词: Animal Experimentation; Animal Model; Animals; Area; Backcrossings; Behavior; Biological Models; Biological Preservation; Biomedical Research; Breeding; Chromosome Mapping; Communities; Complex; Cryopreservation; Data; Databases; Development; Disease; Ensure; Environment; Evolution; Experimental Animal Model; Experimental Models; Expressed Sequence Tags; Female; Fishes; Freezing; Funding; Future; Genetic; Genetic Markers; Genetic Recombination; Genome; Genomics; Germany; Human Resources; Hybrids; Individual; Inheritance Patterns; Insemination; Laboratory Research; Letters; Life; Link; Literature; Maps; Mind; Modeling; Molecular; Monoclonal Antibody R24; Organism; Phenotype; Physiology; Procedures; Production; Protocols documentation; Quantitative Trait Loci; Research; Research Personnel; Research Project Grants; Retrieval; Sampling; Scientist; Source; Specimen; Sperm Motility; Spermatogonia; Synteny; System; Taxon; Testing; Texas; United States; Vertebrates; Xiphophorus; Yang; austin; catalyst; cohort; design; expectation; experience; genetic analysis; interest; life history; male; professor; programs; sperm cell; technology development; tool; trait

DESCRIPTION (provided by applicant): Xiphophorus fishes have been used as an experimental model for fundamental biomedical research for over 75 years. Eventually "mechanistic" hypothesis must be confirmed using intact animal model systems. Well-characterized diverse experimental animal models are best suited to provide this function. In our future scientific environment, diverse animal models that allow us to better understand complex heritable (multigenic) traits will be of considerable importance as we attempt to unravel the genetics underlying complex traits such as behavior and the components leading to alternative in life histories. This R24 proposal is designed to maintain and enhance the Xiphophorus Genetic Stock Center (XGSC). The XGSC has operated within the United States since the 1930's. The traditional strength of the Xiphophorus model involves the non-biased assessment of genetic inheritance patterns associated with complex (multi-genetic) phenotypes within intact animals. The extreme genetic variability among Xiphophorus species (26 known taxa) and capability of producing fertile interspecies hybrids allows chromosomal/marker inheritance to be followed in individual backcross hybrid progeny. Statistical analyses involving hundreds of animals in a cohort can be used to identify markers linked to any trait of interest, so long as the phenotype (as a singular trait or QTL) can be discerned between two parental lines/species and in hybrid progeny. This experimental system is increasingly useful to test hypotheses generated from analyses of whole animal genomes (see 39+ letters of support for the XGSC, appended). With these expectations in mind, it is reasonable to continue to provide pedigreed Xiphophorus fish and associated materials to research laboratories, to continue our progress in development of cryopreservation procedures to ensure long term availability of stocks, and to further develop contemporary genomic tools for research scientists using and/or anticipating use of this valuable vertebrate experimental model. Accordingly our specific aims are: (1) To preserve and enhance the Xiphophorus Genetic Stock Center and provide the research community with animals and materials from genetically managed lines according to the specifications of each investigator. (2) To develop an extensive database of expressed sequence tag (EST) markers. EST marker annotation and the databases will be made available to the community. (3) We will collate the Xiphophorus recombination gene map developed in the previous project period with the BAC minimal tiling path (BAC-MTP) physical map currently being constructed. (4) To continue development of technology allowing cryopreservation of Xiphophorus sperm samples and to provide a preservation program where the XGSC fish lines are maintained as cryopreserved specimens to protect their genetic heritage.

描述（由申请人提供）：Xiphophorus鱼类已被用作基本生物医学研究的实验模型已有75年以上。最终必须使用完整的动物模型系统确认“机械”假设。特征良好的不同实验动物模型最适合提供此功能。在我们未来的科学环境中，随着我们试图揭开诸如行为等复杂特征的基本遗传学的遗传学，使我们能够更好地理解复杂的遗传性（多基因）特征的各种动物模型将非常重要。该R24提案旨在维持和增强Xiphophorus遗传储备中心（XGSC）。自1930年代以来，XGSC一直在美国境内运营。 Xiphophorus模型的传统强度涉及对完整动物中与复合（多遗传）表型相关的遗传遗传模式的无偏见评估。 Xiphophorus物种（26个已知分类单元）的极端遗传变异性以及产生肥沃的种间杂种的能力允许在单个背部杂交杂种后代中遵循染色体/标记遗传。只要可以在两个亲本线/物种之间和混合后代之间识别表型（作为奇异性状或QTL），涉及队列中数百只动物的统计分析可用于识别与任何感兴趣特征相关的标记。该实验系统越来越有用，可用于测试整个动物基因组分析产生的假设（请参见39多张XGSC的支持信，附加）。考虑到这些期望，继续为研究实验室提供建和相关的材料是合理的，继续我们在冷冻保存程序开发方面的进步，以确保股票的长期可用性，并进一步开发当代基因组工具的研究科学家使用和/或预期使用此有价值的脊椎动物实验模型。因此，我们的具体目的是：（1）根据每个研究者的规格，保护和增强Xiphophorus遗传储备中心，并为研究界提供基因管理线的动物和材料。 （2）开发一个表达序列标记（EST）标记的广泛数据库。 EST标记注释和数据库将提供给社区。 （3）我们将与当前正在构建的BAC最小瓷砖路径（BAC-MTP）物理图一起在上一个项目期间开发的Xiphophorus重组基因图。 （4）继续开发技术，允许对Xiphophorus精子样品进行冷冻保存，并提供一个保存计划，其中XGSC鱼类线被维持为冷冻标本，以保护其遗传遗产。