CF 1: Biospecimen Repository Core

CF 1：生物样本存储库核心

基本信息

批准号：
10016092
负责人：
CRISTINA R ANTONESCU
金额：
$ 17.38万
依托单位：
SLOAN-KETTERING INST CAN RESEARCH
依托单位国家：
美国
项目类别：
财政年份：
2018
资助国家：
美国
起止时间：
2018-09-01 至 2023-08-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/10016092
关键词：
Animal Model Animals Apoptosis Applied Research Archives Basic Science Bioinformatics Biological Assay Biological Specimen Banks Blood Cancer Center Cell Line Cell Survival Center Core Grants Clinical Clinical Research Clinical Trials Collaborations Collection Core Facility Custom DNA Data Databases Diagnosis Enrollment Ensure Evaluation Fibrosis Fluorescent in Situ Hybridization Formalin Freezing Generations Genes Human Image Individual Induction of Apoptosis Infrastructure Institutional Review Boards Memorial Sloan-Kettering Cancer Center Microscopic Molecular Morphology Mus Mutation Necrosis Normal Cell Normal tissue morphology Nucleic Acids Oncogenes Paraffin Embedding Pathologic Pathology Patients Pharmaceutical Preparations Pharmacotherapy Phosphorylation Phosphotransferases Pilot Projects Plasma Play Protocols documentation Research Research Personnel Research Project Grants Resources Role Sampling Scanning Services Soft Tissue Neoplasms Soft tissue sarcoma Techniques Testing Tissue Embedding Tissue Microarray Tissue Sample Tissues Translational Research Xenograft Model Xenograft procedure central database cost effective design digital human tissue immunohistochemical markers mouse model mutation screening peripheral blood programs response sarcoma targeted sequencing tumor

项目摘要

CF-1: Biospecimen Repository ABSTRACT The Biospecimen Repository Core (CF-1) is designed to provide support to the basic and translational research efforts of the SPORE. The CF-1 will play a central role in collecting, annotating, storing, distributing, and tracking of tissue and blood biospecimens from patients enrolled in research protocols. The Core will provide SPORE investigators with expert histopathological evaluation of tumor samples both from patients enrolled on research protocols and from murine and xenograft models. Additionally it will assist in selecting appropriate formalin-fixed, paraffin-embedded tissue and snap-frozen tissue for research and pilot projects, as well as enable the creation of tissue microarrays (TMAs) for SPORE investigator studies. The Core will provide assistance in performing and interpreting immunohistochemical studies and fluorescence in situ hybridization (FISH) assays. It will provide scanning and digital archiving of TMA images and reliable interpretation, scoring, and archiving of IHC results from frozen, paraffin-embedded sections or TMA sections. The core will also provide systematic assessment of tumor response to therapy in human tissues and in sarcoma murine models, including interpretation of immunohistochemical markers for drug response, such as changes in kinase phosphorylation, proliferation inhibition, and induction of apoptosis. It also provides a centralized resource for mutational annotation of sarcoma samples and cell lines using the IMPACT platform. Finally, it ensures linkage to the clinical sarcoma database in collaboration with project leaders, the bioinformatics core (CF-2 Qin/Socci) and the administrative core (Singer). This establishes a substantial infrastructure and allows us to provide the required services in a cost-effective manner. The CF-1 is tightly integrated with the MSKCC Institutional Pathology Core (supported by the MSKCC Cancer Center Core Grant) and the Clinical Research Database (CRDB) managed by the administrative core.

CF-1：生物样本库抽象的生物样本存储库核心 (CF-1) 旨在为基础和转化提供支持 SPORE 的研究工作。 CF-1 将在收集、注释、存储、分发和跟踪参与研究方案的患者的组织和血液生物样本。 Core 将为 SPORE 研究人员提供肿瘤样本的专家组织病理学评估均来自参加研究方案的患者以及小鼠和异种移植模型。此外它将有助于选择合适的福尔马林固定、石蜡包埋的组织和速冻组织研究和试点项目，以及为 SPORE 创建组织微阵列 (TMA) 调查员研究。核心将提供表演和口译方面的协助免疫组织化学研究和荧光原位杂交 (FISH) 测定。它将提供 TMA 图像的扫描和数字存档以及 IHC 的可靠解释、评分和存档来自冷冻、石蜡包埋切片或 TMA 切片的结果。核心还将提供系统化的评估人体组织和肉瘤小鼠模型中肿瘤对治疗的反应，包括解释药物反应的免疫组织化学标记物，例如激酶的变化磷酸化、增殖抑制和诱导细胞凋亡。它还提供了一个集中的使用 IMPACT 平台对肉瘤样本和细胞系进行突变注释的资源。最后，它与项目负责人、生物信息学核心（CF-2Qin/Socci）和行政核心（Singer）。这建立了实质性基础设施，使我们能够以具有成本效益的方式提供所需的服务。 CF-1 是与 MSKCC 机构病理学核心紧密集成（由 MSKCC 癌症中心支持）中心核心拨款）和由行政核心管理的临床研究数据库（CRDB）。