Pathology

病理

• 批准号: 7141206
• 负责人: CRISTINA R ANTONESCU
• 金额: $ 9.88万
• 依托单位: SLOAN-KETTERING INST CAN RESEARCH
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-07-01 至 2011-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7141206
• 关键词: neoplasm /cancer; pathology; sarcoma; tissues

The Pathology Core is designed to provide the investigators of the Program Project with expert pathologic evaluation of all soft tissue sarcomas (STS) that present to Memorial Sloan-Kettering Cancer Center. The Core will provide a centralized, comprehensive facility for tissue-based research. The resource will provide tissues and associated data in an equitable and ethical manner, provide professional expertise in histo- and molecular pathology and assist and perform tissue-based experimentation. The human tissues used for research studies are obtained under existing IRB-approved protocols. The specific aims are: 1. To provide consistent, expert pathologic characterization of all STS specimens resected at MSKCC, including a description of gross appearance, histologic type, tumor grade, necrosis, and surgical margins. 2. To supervise the procurement of normal and neoplastic human tissue samples and maintain a bank of frozen sarcoma tissue for special studies conducted under the different projects. This process ensures that adequate amounts of correctly identified and processed tissue reach the appropriate laboratories. 3. To provide expert histologic review for individual projects, including tumor viability and assessment of tumor response to various investigational agents used in different studies, either in post-treated surgical specimens or xenografts. Histologic response assessment to various inhibitors will also be provided for the preclinical in vivo mouse models, as well as histologic expertise for the murine knockout/transgenic models . 4. To assist in performing high-throughput tissue microarray (TMA) and interpreting immunohistochemical (IHC) and in situ hybridization studies (ISH) for validation studies of various microarray projects. An ATA-27 Beecher Automated tissue arrayer will be used to construct TMAs of varying sarcoma types and accompanying normal tissues. Quantitative image analysis of the IHC/ISH results will be performed using an ACIS II (automated cellular imaging system) Chromavision slide scanner tissue arrayer. Assistance with the development of the Clinical Tissue Microarray (ClinTMA) Database by the Bioinformatics Core will be provided, and results will be entered into this database as continuous values. These aims offer a substantial infrastructure to the STS Program Project and allows Core B to provide the required services in a cost effective manner.

病理学核心旨在为该项目的研究人员提供专家 对纪念斯隆-凯特琳癌症中心出现的所有软组织肉瘤 (STS) 进行病理学评估 中心。该核心将为基于组织的研究提供集中、综合的设施。该资源将 以公平和道德的方式提供组织和相关数据，提供专业知识 组织和分子病理学，并协​​助和进行基于组织的实验。人体组织 用于研究的数据是根据现有的 IRB 批准的协议获得的。具体目标是： 1. 为 MSKCC 切除的所有 STS 标本提供一致、专业的病理特征， 包括大体外观、组织学类型、肿瘤分级、坏死和手术切缘的描述。 2. 监督正常和肿瘤人体组织样本的采购并维护样本库 冷冻肉瘤组织用于不同项目下进行的特殊研究。这个过程确保 足够数量的正确识别和处理的组织到达适当的实验室。 3. 为个别项目提供专家组织学审查，包括肿瘤活力和评估 肿瘤对不同研究中使用的各种研究药物的反应，无论是在治疗后的手术中 标本或异种移植物。还将提供对各种抑制剂的组织学反应评估 临床前体内小鼠模型，以及小鼠敲除/转基因模型的组织学专业知识。 4. 协助进行高通量组织微阵列（TMA）和解读免疫组化 (IHC) 和原位杂交研究 (ISH) 用于各种微阵列项目的验证研究。 ATA-27 Beecher 自动组织阵列仪将用于构建不同肉瘤类型和 伴随正常组织。 IHC/ISH 结果的定量图像分析将使用 ACIS II（自动细胞成像系统）Chromavision 玻片扫描仪组织阵列仪。协助 生物信息学核心开发临床组织微阵列（ClinTMA）数据库将 提供，结果将作为连续值输入到该数据库中。 这些目标为 STS 计划项目提供了坚实的基础设施，并允许核心 B 提供 以具有成本效益的方式提供所需的服务。