C1 - Biospecimen Repository Core

C1 - 生物样本存储库核心

基本信息

批准号：
7976119
负责人：
CRISTINA R ANTONESCU
金额：
$ 7.59万
依托单位：
SLOAN-KETTERING INST CAN RESEARCH
依托单位国家：
美国
项目类别：
财政年份：
2010
资助国家：
美国
起止时间：
2010-07-01 至 2015-06-30
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/7976119
关键词：
Animal Model Animals Apoptosis Archives Area Biological Assay Cell Line Clinical Clinical Data Collection Complement Complement component C1s Core Facility DNA Data Databases Diagnosis Dideoxy Chain Termination DNA Sequencing Drug resistance Enrollment Evaluation Excision Fibrosis Formalin Freezing Genotype Goals Housing Human Image Immunohistochemistry In Situ Individual Laboratories Lesion Microscopic Molecular Monitor Morphology Mus Necrosis Needle biopsy procedure Normal tissue morphology Nucleic Acids Oncogenes Paraffin Embedding Pathologic Pharmaceutical Preparations Phosphotransferases Pilot Projects Play Preparation Procedures Protocols documentation Reproduction spores Research Research Personnel Research Project Grants Resources Role Sampling Scanning Serum Soft Tissue Neoplasms Specimen System Tissues Tumor Cell Line Update Xenograft Model chemotherapy design digital human tissue oncology peripheral blood programs repository response sarcoma soft tissue tumor

项目摘要

The Biospecimen Repository Core (CF-A) is designed to provide support to the basic translafional research efforts of the SPORE. The Core will play a central role in collecfing, annotafing, storing, distribufing, and tracking sarcoma and normal tissue blospecimens from pafients enrolled in research protocols. The four key focus areas forthe sarcoma SPORE Biospecimen Repository are high quality banked fissue (frozen and paraffin-embedded) and blood (serum and plasma); accurate and updated procedural, pathologic, and clinical annotafion; efficient and effective distribufion of blospecimens for use in research protocols; and a robust tracking system capable of monitoring blospecimens from collecfion through utilization. Integrating these four components is a major goal of the Biospecimen Repository Core. The Core will provide SPORE invesfigators with expert histopathologic evaluation of tumor samples, both from pafients enrolled on research protocols and from murine and xenograft models. The Core will provide assistance in performing and interprefing immunohistochemical studies, in selecfing fissue for microdissecfion and construcfion of arrays, and in collaborating with project leaders and CF-B. The core will also provide systematic assessment of tumor response to therapy in human tissues and in animal models of sarcomas, including interpretafion of immunohistochemical markers for drug response, such as changes in kinase phosphorylafion, inhibifion of proliferafion, and inducfion of apoptosis. The Core will provide mutafional annotafion for sarcoma tumors and cell lines, as needed by individual research projects.

生物样本库核心（CF-A）旨在为基础转化研究提供支持 SPORE的努力。核心将在收集、注释、存储、分发和处理方面发挥核心作用。跟踪参加研究方案的患者的肉瘤和正常组织样本。四个关键肉瘤 SPORE 生物样本库的重点领域是高质量的储存组织（冷冻和石蜡包埋）和血液（血清和血浆）；准确和更新的程序、病理和临床注释；高效且有效地分发用于研究方案的样本；和一个强大的跟踪系统，能够监控样本从采集到使用的整个过程。整合这四个组成部分是生物样本存储库核心的主要目标。 Core 将为 SPORE 研究人员提供肿瘤样本的专家组织病理学评估，包括来自参加研究方案的患者以及小鼠和异种移植模型。核心将提供协助进行和解释免疫组织化学研究，选择组织显微切割和阵列构建，并与项目负责人和 CF-B 合作。核心将还提供对人体组织和动物模型中肿瘤治疗反应的系统评估肉瘤，包括药物反应的免疫组织化学标记物的解释，例如激酶磷酸化、增殖抑制和细胞凋亡诱导。核心将提供根据个别研究项目的需要，对肉瘤肿瘤和细胞系进行突变注释。