BIOLOGICAL RESEARCH CORE

生物研究核心

基本信息

批准号：
7915749
负责人：
GABRIELLA CS-SZABO
金额：
$ 18.73万
依托单位：
RUSH UNIVERSITY MEDICAL CENTER
依托单位国家：
美国
项目类别：
财政年份：
2009
资助国家：
美国
起止时间：
2009-09-01 至 2011-08-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/7915749
关键词：
Academic Medical Centers Address Aftercare Alginates Animal Husbandry Animals Biochemical Biochemistry Cataloging Catalogs Cattle Cell Culture Techniques Cell Separation Cells Collection Computers Core Facility Data Databases Detection Development Funding Goals Histology Hour Human Human Resources Individual Intervertebral disc structure Laboratories Magnetic Resonance Imaging Methodology Methods Molecular Biology Techniques Operating Rooms Organ Culture Techniques Oryctolagus cuniculus Persons Primary Cell Cultures Procedures Program Research Project Grants RNA Records Regulation Research Personnel Research Project Grants Resources Safety Sampling Services Special Equipment System Tail Test Result Testing Time Tissue Engineering Tissue Procurements Tissue Sample Tissues Vertebral column Work animal care biological research cost data management evaluation/testing experience flexibility human tissue intervertebral disk degeneration investigator training meetings multidisciplinary programs repository research study success synergism tissue/cell culture

项目摘要

The Biological Research Core (BRC) will provide all four Research Projects with technical and personnel support for facilitating, standardizing, and implementing the collection, management and analysis of tissue samples. The BRC will support investigators by providing animals for individual experiments, collecting animal and human tissue samples and performing standardized testing and data management (record keeping). The BRC intends to aid each project by providing technical, instrumental and personnel support which are (i) commonly used by several projects, or (ii) occasionally used by investigators but require special technical or safety regulations, or (iii) require special equipment or technical experience. The BRC facility is composed of three major Core units. The Animal Care Core Unit keeps rabbits, performs pretreatment and post-treatments of animals that are used for the experiments, as well as sacrifices animals and collects and distributes tissue samples. The Tissue Procurement and Sample Analysis Core Unit will support the Projects by collecting human intervertebral discs from cadaveric donors and from the operating rooms of Rush University Medical Center. This Unit will perform MRI on human tissues for the detection of disc degeneration. Disc samples then will be evaluated and prepared for specialized biochemical analyses, histology tests, and prepared for storage. The Cell and Tissue Culture Core Unit will prepare tissues for cell isolation and culturing. This Unit will generate and maintain a computer database to catalog all animals, and animal and human tissues with their appropriate test records. The BRC will provide an economy of effort and reduce the overall cost of individual projects while creating a higher level of cooperation among the individual projects. The coordination and integration of the tissue appropriation, culture facilities and histology and biochemistry laboratories creates more flexibility and efficiency for allocating laboratory, technical and personnel resources to support the individual Projects and address the urgent needs of individual projects at critical times. The success of a multidisciplinary core facility, due to its service function is less noticeable than that of the individual research projects. However, without this facility, the individual projects would all spend more time and effort in performing the same tasks, e.g. organizing tissue procurement, analyzing tissues and establishing/developing new methods.

生物研究核心（BRC）将为所有四个研究项目提供技术和人员支持促进、标准化和实施组织的收集、管理和分析样品。 BRC 将通过提供动物进行个体实验、收集动物和人体组织样本并进行标准化检测和数据管理（记录保持）。 BRC打算通过提供技术、仪器和人员支持来帮助每个项目 (i) 多个项目常用，或 (ii) 研究者偶尔使用，但需要特殊技术或安全法规，或 (iii) 需要特殊设备或技术经验。 BRC 设施是由三大核心单元组成。动物护理核心部门饲养兔子，进行预处理和用于实验的动物的后处理，以及处死动物并收集和分发组织样本。组织采购和样本分析核心部门将支持通过从尸体捐赠者和手术室收集人类椎间盘的项目拉什大学医学中心。该装置将对人体组织进行 MRI 以检测椎间盘退化。然后将评估并准备光盘样本以进行专门的生化分析，组织学检测，并准备保存。细胞和组织培养核心单元将为细胞准备组织隔离和培养。该部门将生成并维护一个计算机数据库来对所有动物进行分类，以及动物和人体组织及其适当的测试记录。 BRC 将提供经济的努力并降低单个项目的总体成本，同时创建一个各个项目之间的合作水平更高。组织的协调与整合拨款、培养设施以及组织学和生化实验室创造了更大的灵活性和分配实验室、技术和人力资源以支持各个项目的效率解决个别项目在关键时刻的紧急需求。多学科核心设施的成功，由于其服务功能不如综合设施那么引人注目。个人研究项目。然而，如果没有这个设施，各个项目都会花费更多的时间以及执行相同任务的努力，例如组织组织采购、分析组织和建立/开发新方法。