CORE--ANIMAL AND TISSUE

核心——动物和组织

• 批准号: 6217142
• 负责人: GABRIELLA CS-SZABO
• 金额: $ 22.63万
• 依托单位: RUSH UNIVERSITY MEDICAL CENTER
• 依托单位国家: 美国
• 财政年份: 1999
• 资助国家: 美国
• 起止时间: 1999-07-01 至 2000-02-29
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6217142
• 关键词: animal care; arthritis; articular cartilage; autoimmune disorder; biomedical facility; data management; histology; human tissue; immunization; laboratory mouse; sample collection; tissue /cell culture

The Animal and Tissue Core (ATC) will provide technical and personnel support for facilitating, standardizing, and implementing the collection, management and analysis of tissue samples. The ATC will support investigators by providing animals pretreated for individual experiments, collecting tissue samples and performing standardized testing and data management (record keeping). This Core Facility will serve all investigators who will be needing assistance with (1) animal care and treatments, (2) collection of tissue and/or body fluid, (3) cell and tissue culture methods, (4) standardized testing for assessment of the development and progression of arthritis, and (5) histological evaluation of tissue sample. The ATC intends to aid each project by providing technical, instrumental and personnel support which are (i) commonly used by several projects or (ii) occasionally used by investigators but require special technical or safety regulations or (iii) require special equipment or technical expertise. The ACT facility, based one the expected demands of the this application, will consist of three units. The Animal Care Core Unit will collect human cartilage, prepare antigens for immunization, and immunize animals according to the needs of the investigators. Thus, this Unit will serve all projects with pre-arthritic and/or arthritic animals. The Tissue Appropriation and Histology unit will be responsible for sample storage, record keeping and data management, and performing histological evaluation of the skeletal tissues with their appropriate test records (data collected from the other two Core Units and the individual research Projects). Thus, this Core facility will help investigators collect tissues for specialized analysis, perform histology tests, and store tissue samples for further analyses. The Cell and Tissue Culture Core Unit will standardize and perform tests required by the different projects, measure serum antibody levels (B-cell response) and test cytokine response by antigen/mitogen stimulated spleen cells (T-cell activity). Thus, this Core Unit will perform tests most appropriate for the individual needs of the projects. Since many of these techniques frequently require combinations of different methods and protocols, we intend to centralize these three Core Units into one Core facility directed and coordinated by one person, to guarantee the most effective and efficient integration of research approaches.

动物和组织核心（ATC）将提供技术和人员 支持促进、标准化和实施 组织样本的收集、管理和分析。空中交通管制将 通过提供针对个体进行预处理的动物来支持研究人员 实验，收集组织样本并进行标准化 测试和数据管理（记录保存）。该核心设施将 为所有需要 (1) 动物帮助的调查人员提供服务 护理和治疗，(2) 组织和/或体液的收集，(3) 细胞和组织培养方法，（4）标准化测试评估 关节炎的发生和进展，以及（5）组织学 组织样本的评估。 ATC 打算通过以下方式帮助每个项目： 提供技术、工具和人员支持，其中 (i) 多个项目普遍使用或 (ii) 偶尔使用 调查人员但需要特殊的技术或安全法规或 (iii) 需要特殊设备或技术专长。澳大利亚首都领地 根据该应用程序的预期需求，设施将 由三个单元组成。动物护理核心单位将收集人类 软骨，制备免疫抗原，并对动物进行免疫 根据调查人员的需要。因此，该单位将服务于 所有涉及关节炎前期和/或关节炎动物的项目。组织 拨款和组织学部门将负责样品储存， 记录保存和数据管理，并进行组织学检查 通过适当的测试记录评估骨骼组织 （从其他两个核心单位和个人收集的数据 研究项目）。因此，这个核心设施将帮助调查人员 收集组织进行专门分析，进行组织学测试，以及 储存组织样本以供进一步分析。细胞和组织培养 核心单元将标准化并执行不同要求的测试 项目、测量血清抗体水平（B 细胞反应）和测试 抗原/丝裂原刺激的脾细胞（T 细胞）产生细胞因子反应 活动）。因此，该核心单元将执行最适合的测试 项目的个性化需求。由于许多这些技术 经常需要不同方法和协议的组合，我们 打算将这三个核心单位集中到一个核心设施中 由一人指导和协调，以保证最有效的 研究方法的有效整合。