Interdepartmental Two-photon Imaging Center

跨部门双光子成像中心

• 批准号: 7816800
• 负责人: DALTON JAMES SURMEIER
• 金额: $ 59.62万
• 依托单位: NORTHWESTERN UNIVERSITY AT CHICAGO
• 依托单位国家: 美国
• 财政年份: 2007
• 资助国家: 美国
• 起止时间: 2007-05-01 至 2012-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7816800
• 关键词: Interdepartmental; Two; photon; Imaging; Center

Cellular and molecular imaging technologies are revolutionizing neuroscience. The technology with the biggest potential impact on this field is "non-linear optical microscopy" which enables two-photon (2P) imaging of fluorescent structures deep in living tissue with unprecedented spatial and temporal resolution. Combining 2P imaging with electrophysiology and molecular uncaging creates an extraordinarily powerful tool that is having a profound effect on the conduct of neuroscientific study. The strategic goal of this revised proposal is to put this critical technology in the hands of a two highly productive groups of neuroscientists at Northwestern University (NU) that receive nearly $8M/year (direct costs) in funding from NINDS. One of these groups is focused on the properties of neuronal dendrites in health, aging and disease; the other group is focused on neural stem cell biology and its application to neuroregeneration. To maximize the investment of NU and NINDS in these research programs, both groups need ready access to user-friendly, multifunctional 2P imaging workstations. The following specific aims are designed to achieve this goal: 1. to further characterize and optimize the performance of our existing 2P imaging workstations; 2. to extend the capabilities of the existing workstations by adding new hardware and software features integrating electrophysiology and molecular uncaging; 3. to create additional core facilities with 2P imaging workstations and technical support for NIH funded investigators who do not currently have access to these resources; 4. to establish an infrastructure that ensures the safe, efficient and productive operation of all the cores; 5. to create an Internet-based distribution point for dissemination of information about applications, software and hardware design/implementation A corporate partner with expertise in the production of 2P workstations, Prairie Technologies, has been recruited to help achieve these aims. By establishing a dialogue between industry and researchers, we hope to accelerate the development of hardware and software that best meets the needs of the neuroscience community. The attainment of these aims will not only have a transforming impact on the NIH funded research programs at NU but it will significantly accelerate the delivery of these technologies to the broader neuroscience community, quicken the pace of scientific discovery and promote the development of new treatments for neurological disorders.

细胞和分子成像技术正在彻底改变神经科学。潜在影响最大的技术是“非线性光学显微镜”，该技术可以对具有前所未有的空间和时间分辨率的生物组织深处的荧光结构进行两光（2p）成像。将2P成像与电生理学和分子渗透结合起来创造了一种非常强大的工具，该工具对神经科学研究的行为产生了深远的影响。这项修订的提案的战略目标是将这项关键技术置于西北大学（NU）的两个高产神经科学家群体的手中，该群体获得了近800万美元/年（直接成本）的NINDS资金。这些群体之一的重点是神经元树突在健康，衰老和疾病中的特性。另一组专注于神经干细胞生物学及其在神经变成中的应用。 为了最大程度地提高NU和NINDS在这些研究计划中的投资，两组都需要准备好访问用户友好的多功能2P成像工作站。以下特定目标旨在实现这一目标： 1。进一步表征和优化我们现有的2P成像工作站的性能； 2。通过添加集成电生理学和分子渗透的新硬件和软件来扩展现有工作站的功能； 3。用2P成像工作站创建其他核心设施，并为NIH资助的调查人员提供技术支持，这些调查员目前无法访问这些资源； 4。建立一个基础设施，以确保所有核心的安全，高效和富有成效的运作； 5。为了创建一个基于Internet的分销点，用于传播有关应用程序，软件和硬件设计/实施的信息，该公司的合作伙伴具有生产2P工作站的专业知识，Prairie Technologies已被招募，以帮助实现这些目标。通过建立行业与研究人员之间的对话，我们希望加快最能满足神经科学社区需求的硬件和软件的开发。 实现这些目标不仅会对NU的NIH资助研究计划产生转变的影响，而且还将大大加速这些技术向更广泛的神经科学社区的传递，加快科学发现的步伐，并促进神经系统疾病的新治疗方法的发展。