Interdepartmental Two-photon Imaging Center

跨部门双光子成像中心

• 批准号: 8145904
• 负责人: DALTON JAMES SURMEIER
• 金额: $ 35.04万
• 依托单位: NORTHWESTERN UNIVERSITY AT CHICAGO
• 依托单位国家: 美国
• 财政年份: 2007
• 资助国家: 美国
• 起止时间: 2007-05-01 至 2012-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8145904
• 关键词: Aging; Communities; Computer software; Core Facility; Dendrites; Development; Direct Costs; Disease; Electrophysiology (science); Ensure; Funding; Goals; Hand; Health; Image; Imaging technology; Industry; Information Dissemination; Internet; Investments; Life; Microscopy; Molecular; National Institute of Neurological Disorders and Stroke; Neurons; Neurosciences; Optics; Performance; Production; Property; Recruitment Activity; Research; Research Infrastructure; Research Personnel; Resolution; Resources; Structure; Technology; Tissues; United States National Institutes of Health; Universities; base; design; meetings; molecular imaging; nerve stem cell; nervous system disorder; operation; programs; stem cell biology; tool; two-photon; user-friendly

Cellular and molecular imaging technologies are revolutionizing neuroscience. The technology with the biggest potential impact on this field is "non-linear optical microscopy" which enables two-photon (2P) imaging of fluorescent structures deep in living tissue with unprecedented spatial and temporal resolution. Combining 2P imaging with electrophysiology and molecular uncaging creates an extraordinarily powerful tool that is having a profound effect on the conduct of neuroscientific study. The strategic goal of this revised proposal is to put this critical technology in the hands of a two highly productive groups of neuroscientists at Northwestern University (NU) that receive nearly $8M/year (direct costs) in funding from NINDS. One of these groups is focused on the properties of neuronal dendrites in health, aging and disease; the other group is focused on neural stem cell biology and its application to neuroregeneration. To maximize the investment of NU and NINDS in these research programs, both groups need ready access to user-friendly, multifunctional 2P imaging workstations. The following specific aims are designed to achieve this goal: 1. to further characterize and optimize the performance of our existing 2P imaging workstations; 2. to extend the capabilities of the existing workstations by adding new hardware and software features integrating electrophysiology and molecular uncaging; 3. to create additional core facilities with 2P imaging workstations and technical support for NIH funded investigators who do not currently have access to these resources; 4. to establish an infrastructure that ensures the safe, efficient and productive operation of all the cores; 5. to create an Internet-based distribution point for dissemination of information about applications, software and hardware design/implementation A corporate partner with expertise in the production of 2P workstations, Prairie Technologies, has been recruited to help achieve these aims. By establishing a dialogue between industry and researchers, we hope to accelerate the development of hardware and software that best meets the needs of the neuroscience community. The attainment of these aims will not only have a transforming impact on the NIH funded research programs at NU but it will significantly accelerate the delivery of these technologies to the broader neuroscience community, quicken the pace of scientific discovery and promote the development of new treatments for neurological disorders.

细胞和分子成像技术正在彻底改变神经科学。对该领域具有最大潜在影响的技术是“非线性光学显微镜”，它能够以前所未有的空间和时间分辨率对活体组织深处的荧光结构进行双光子（2P）成像。将 2P 成像与电生理学和分子解禁相结合创造了一种非常强大的工具，对神经科学研究的进行产生了深远的影响。此修订提案的战略目标是将这项关键技术交到西北大学 (NU) 的两个高产神经科学家小组手中，这两个小组每年从 NINDS 获得近 800 万美元（直接成本）的资助。其中一组专注于神经元树突在健康、衰老和疾病中的特性；另一组专注于神经干细胞生物学及其在神经再生中的应用。 为了最大限度地提高 NU 和 NINDS 在这些研究项目中的投资，两个团队都需要随时访问用户友好的多功能 2P 成像工作站。为实现这一目标，设计了以下具体目标： 1. 进一步表征和优化我们现有2P成像工作站的性能； 2. 通过添加集成电生理学和分子解禁的新硬件和软件功能来扩展现有工作站的功能； 3. 创建额外的核心设施，配备 2P 成像工作站，并为目前无法获得这些资源的 NIH 资助的研究人员提供技术支持； 4. 建立确保所有核心安全、高效和高效运行的基础设施； 5. 创建一个基于互联网的分发点，用于传播有关应用程序、软件和硬件设计/实施的信息 为了帮助实现这些目标，我们聘请了一家在 2P 工作站生产方面具有专业知识的企业合作伙伴 Prairie Technologies。通过在行业和研究人员之间建立对话，我们希望加速最能满足神经科学界需求的硬件和软件的开发。 这些目标的实现不仅会对 NIH 资助的 NU 研究项目产生变革性影响，还将显着加快这些技术向更广泛的神经科学界的交付，加快科学发现的步伐，并促进新疗法的开发。神经系统疾病。