Development of Human-Selective Nerve Illumination Peptide for Surgery

开发用于手术的人类选择性神经照明肽

基本信息

批准号：
9788444
负责人：
Quyen Nguyen
金额：
$ 14.91万
依托单位：
UNIVERSITY OF CALIFORNIA, SAN DIEGO
依托单位国家：
美国
项目类别：
财政年份：
2018
资助国家：
美国
起止时间：
2018-09-20 至 2020-06-30
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/9788444
关键词：
Amino Acids Anatomy Animals Binding Clinical Trials Clinical trial protocol document Color Development Disease Dissection Distal Dose Dyes Excision Facial nerve structure Fluorescein Fluorescence Fluorescent Dyes Formulation Generations Glycoproteins Goals Human Imagery Infection Injury Investigational Drugs Investigational New Drug Application Label Laboratories Ligands Light Lighting Link Molecular Molecular Target Morbidity - disease rate Motor Nerve Operating Rooms Operative Surgical Procedures Outcome Parotid Neoplasms Patient-Focused Outcomes Patients Peptides Peripheral Peripheral Nerves Phage Display Pharmacology Pharmacology and Toxicology Phase I Clinical Trials Postoperative Period Procedures Publications Radical Prostatectomy Rodent Salivary Gland Neoplasms Singlet Oxygen Specificity Structure Structure-Activity Relationship Surgeon Testing Texture Time TimeLine Tissues Toxic effect Toxicology Trauma Tumor Cell Invasion United States Food and Drug Administration Variant Vertebral column Visual Work afferent nerve autonomic nerve base clinical development clinical translation cyanine dye 5 experience human imaging image guided imaging agent improved neoplasm surgery nerve injury novel off-label use phase 1 testing pre-clinical preservation prevent protein aminoacid sequence reconstruction repaired tool

项目摘要

Abstract A fundamental goal of surgery is function preservation and minimizing patient morbidity. Current nerve identification during surgery utilizes non-quantifiable criteria such as anatomy, texture, color, relationship to surrounding structures to distinguish nerves from non-nerve tissues. In instances of trauma, tumor invasion or infection, nerve identification using the above criteria may be challenging. Using white light reflectance, which is the standard mode of illumination in operating rooms, the visual difference between small nerves, such as cavernosal nerves important during radical prostatectomy or distal branches of the facial nerve important during surgery for salivary gland neoplasms, and adjacent tissue can be imperceptible. Our laboratory has extensive experience in developing molecularly targeted smart probes for surgical visualization of nerves. Recently, we have identified a novel peptide (HNP401) via phage display to have high level of human nerve binding. In this proposal, we aim to optimize HNP401 to enable its clinical translation for human surgery.

抽象的手术的基本目标是保留功能并尽量减少患者发病率。当前手术期间的神经识别利用不可量化的标准，例如解剖学、纹理、颜色、与周围结构的关系，以区分神经和非神经组织。在外伤、肿瘤侵袭或感染的情况下，使用上述标准进行神经识别可能是具有挑战性的。采用白光反射，这是操作中的标准照明模式房间里，小神经之间的视觉差异，例如海绵体神经，在根治性前列腺切除术或面神经远端分支在唾液腺手术中很重要肿瘤和邻近组织可能难以察觉。我们的实验室在以下方面拥有丰富的经验开发用于神经外科可视化的分子靶向智能探针。最近，我们有通过噬菌体展示鉴定出一种新型肽（HNP401），该肽具有高水平的人类神经结合能力。在在该提案中，我们的目标是优化 HNP401，使其能够应用于人体手术的临床转化。