Testing Surgery Guided by Molecular Fluorescence Imaging

分子荧光成像引导的测试手术

基本信息

批准号：
7667201
负责人：
Quyen Nguyen
金额：
$ 14.27万
依托单位：
UNIVERSITY OF CALIFORNIA, SAN DIEGO
依托单位国家：
美国
项目类别：
财政年份：
2007
资助国家：
美国
起止时间：
2007-09-20 至 2012-07-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/7667201
关键词：
Advisory Committees Animal Model Animals Biopsy Blood Vessels California Cells Cellular biology Clinical Medicine Color Coupled Development Doctor of Philosophy Excision Fellowship Filtration Fluorescence Fluorescent Dyes Fluorescent Probes Future Head and Neck Surgery Human Resources Image Image-Guided Surgery Imagery Immune Immunocompetent Immunocompromised Host Individual Label Light Location Magnetic Resonance Imaging Malignant Neoplasms Mentors Metalloproteases Methods Modality Modeling Molecular Monitor Mus Neoplasm Metastasis Neoplasm Transplantation Nerve Tissue Neurobiology Normal tissue morphology Nude Mice Operative Surgical Procedures Optics Otolaryngology Patients Peptides Pharmacology Physicians Positron-Emission Tomography Postdoctoral Fellow Principal Investigator Process Program Development Radioactive Recurrence Reporting Research Research Personnel Resected Residencies Residual Tumors Resources Scientist Site Solid Solid Neoplasm Source Specificity Specimen Structure Surgeon Surgical Models Surgical margins System Testing Texture Time Training Training Programs Transgenic Organisms Transplantation Tumor Tissue Universities Washington Xenograft procedure animal model development base career cyanine dye 5 feeding fluorescence imaging fluorophore graduate student innovation minimally invasive molecular marker noninvasive diagnosis novel professor programs skull base software systems tumor whole body imaging

项目摘要

DESCRIPTION (provided by applicant): This proposal describes a 5 year training program for the development of academic career in Surgery. The principal investigator has completed a combined M.D./Ph.D. program at the Washington University and an ACGME accredited residency training program in Otolaryngology-Head and Neck Surgery at the University of California San Diego (UCSD). She has also completed a postdoctoral fellowship in Skull Base Surgery at UCSD and now, will expand her combined training in clinical medicine and scientific research through an unique integration of interdepartmental expertise and resources. Dr. Roger Tsien will mentor the principal investigator's scientific development. Dr. Tsien is a recognized leader in the field of cell biology and neurobiology and is world renowned for developing colorful fluorescent dyes for monitoring intracellular processes. He has been a Howard Hughes Investigator since 1989, is a Professor in the Dept of Pharmacology at UCSD and has trained numerous postdoctoral fellows and graduate students. To enhance the training, the program will also have an advisory committee of physician scientists who are leaders in their fields and who will provide scientific and career advice. Research will focus on the development of an animal model for molecular fluorescence guided surgery. The specific aims include 1) develop an imaging system for fluorescence guided surgery in small animals, 2) develop animal models for fluorescence-guided surgery, with quantitative objective criteria for accuracy of excision 3) develop animal models for whole body imaging coupled with fluorescence image guided surgery Despite recent technological advances in the noninvasive diagnosis of cancers including CT, MRI and PET imaging, the main treatment modality for solid malignant tumors is surgical excision. However, surgical excision of tumors remains dependent on the individual surgeon's ability to differentiate tumor from normal tissue using non-quantifiable criteria such as tumor location, texture, color, relationship to surrounding structures etc. The relevance of this project is to provide the operating surgeon with a sensitive molecular marker visualizable in real time to differentiate tumors from non-tumor tissue intraoperatively, thereby guiding surgical excision.

描述（由申请人提供）：该提案描述了一个为期 5 年的外科学术职业发展培训计划。主要研究者已完成医学博士/博士学位。华盛顿大学的项目和加州大学圣地亚哥分校 (UCSD) 的 ACGME 认可的耳鼻喉头颈外科住院医师培训项目。她还完成了加州大学圣地亚哥分校颅底外科的博士后研究，现在将通过跨部门专业知识和资源的独特整合来扩展她在临床医学和科学研究方面的综合培训。 Roger Tsien 博士将指导首席研究员的科学发展。钱博士是细胞生物学和神经生物学领域公认的领导者，因开发用于监测细胞内过程的彩色荧光染料而闻名于世。他自 1989 年起担任霍华德休斯研究员，现任加州大学圣地亚哥分校药理学系教授，培养了众多博士后和研究生。为了加强培训，该计划还将设立一个由医学科学家组成的咨询委员会，他们是各自领域的领导者，将提供科学和职业建议。研究将集中于分子荧光引导手术动物模型的开发。具体目标包括 1) 开发用于小动物荧光引导手术的成像系统，2) 开发荧光引导手术的动物模型，并为切除的准确性制定定量客观标准 3) 开发结合荧光图像的全身成像动物模型引导手术尽管最近在癌症无创诊断（包括 CT、MRI 和 PET 成像）方面取得了技术进步，但实体恶性肿瘤的主要治疗方式是手术切除。然而，肿瘤的手术切除仍然取决于个体外科医生使用不可量化的标准（例如肿瘤位置、纹理、颜色、与周围结构的关系等）区分肿瘤与正常组织的能力。该项目的相关性是为手术外科医生提供指导具有实时可视化的敏感分子标记，可在术中区分肿瘤与非肿瘤组织，从而指导手术切除。